High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai

Nakayama, Jun; Saito, Ryoichi; Hayashi, Yusuke; Kitada, Nobuo; Tamaki, Shota; Han, Yuxuan; Semba, Kentaro; Maki, Shôjirô

doi:10.3390/ijms21217896

Cited by 22 publications

(25 citation statements)

References 18 publications

(28 reference statements)

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“…Two characteristics of TokeOni were improved by seMpai; since the pH value of TokeOni is acidic [ 89 ], acidosis is a potential problem, and hepatic background signals are detected when using TokeOni [ 89 , 106 ] but not when using DLH2. Furthermore, seMpai is a luciferin analogue designed with containing N-atom in the AkaLumine moiety, slightly changing its bioluminescence reaction with Fluc and AkaLuc ( K m = 6.2 and 44.9 µM, respectively in Table 2 ).…”

Section: Chemical and Physical Characteristics Of The Nir Luciferimentioning

confidence: 99%

“…Moreover, seMpai allows high-sensitivity detection in in vivo BLI, and it can be used to detect pulmonary micro-metastases. In addition, seMpai is highly soluble (69 mM) in PBS (pH 7.4) [ 82 ] and does not produce hepatic background signals in breast cancer metastasis models [ 89 ]. However, seMpai BLI cannot detect biological events at single-cell-level resolutions, like AkaBLI.…”

Section: Chemical and Physical Characteristics Of The Nir Luciferimentioning

confidence: 99%

“…The fact that bioluminescence patterns change depending on the tissue permeability of the luciferin analogues used is a serious problem because it means that the chemical and physical properties of luciferin analogues have significant effects on biological discovery. Nevertheless, since seMpai BLI reduces hepatic background signals, it is very useful for the study of systemic mouse models, such as those used to study metastasis [ 89 ] ( Figure 2 ). Thus, it is important that researchers fully understand the characteristics of their chosen in vivo BLI system when applying it to their studies.…”

Section: Chemical and Physical Characteristics Of The Nir Luciferimentioning

confidence: 99%

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How to Select Firefly Luciferin Analogues for In Vivo Imaging

Saito-Moriya

Nakayama²,

Kamiya

et al. 2021

IJMS

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Bioluminescence reactions are widely applied in optical in vivo imaging in the life science and medical fields. Such reactions produce light upon the oxidation of a luciferin (substrate) catalyzed by a luciferase (enzyme), and this bioluminescence enables the quantification of tumor cells and gene expression in animal models. Many researchers have developed single-color or multicolor bioluminescence systems based on artificial luciferin analogues and/or luciferase mutants, for application in vivo bioluminescence imaging (BLI). In the current review, we focus on the characteristics of firefly BLI technology and discuss the development of luciferin analogues for high-resolution in vivo BLI. In addition, we discuss the novel luciferin analogues TokeOni and seMpai, which show potential as high-sensitivity in vivo BLI reagents.

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Section: Chemical and Physical Characteristics Of The Nir Luciferimentioning

confidence: 99%

Section: Chemical and Physical Characteristics Of The Nir Luciferimentioning

confidence: 99%

Section: Chemical and Physical Characteristics Of The Nir Luciferimentioning

confidence: 99%

See 1 more Smart Citation

How to Select Firefly Luciferin Analogues for In Vivo Imaging

Saito-Moriya

Nakayama²,

Kamiya

et al. 2021

IJMS

Self Cite

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show abstract

“…Optical techniques are subject to attenuation at depth due to absorption and scattering of light, and thus superficial tumors are most easily detected, specifically subcutaneous, or at disease sites near the surface such as mammary fat pad (Figure 3). However, light can penetrate several millimeters and effective signal is observed by BLI in deeper tissues such as the lungs [180,[187][188][189], prostate [190][191][192], brain [193,194], pancreas [195,196], liver [197,198], head and neck [113], bone [199,200] and kidney [136,201] in mice. The most common implementation of BLI in oncology is simply to relate signal intensity to tumor burden and indeed several studies have shown that there is a strong correlation for untreated control tumors up to about 2 cm 3 [89,202].…”

Section: Atp + D-luciferin + O2 -----------Luciferase ---------------mentioning

confidence: 99%

Non-Invasive Evaluation of Acute Effects of Tubulin Binding Agents: A Review of Imaging Vascular Disruption in Tumors

Liu¹,

O'Kelly²,

Schuetze³

et al. 2021

Preprint

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Tumor vasculature proliferates rapidly, generally lacks pericyte coverage, and is uniquely frag-ile making it an attractive therapeutic target. A subset of small-molecule tubulin binding agents cause disaggregation of the endothelial cytoskeleton leading to enhanced vascular permeability generating increased interstitial pressure. The resulting vascular collapse and ischemia cause downstream hypoxia, ultimately leading to cell death and necrosis. Thus, local damage gener-ates massive amplification and tumor destruction. The tumor vasculature is readily accessed and potentially a common target irrespective of disease site in the body. Development of a therapeutic approach and particularly next generation agents benefits from effective non-invasive assays. Imaging technologies offer varying degrees of sophistication and ease of implementation. This review considers technological strengths and weaknesses with examples from our own laboratory. Methods reveal vascular extent and patency, as well as insights into tissue viability, proliferation and necrosis. Spatiotemporal resolution ranges from cellular mi-croscopy to single slice tomography and full three-dimensional views of whole tumors and measurements can be sufficiently rapid to reveal acute changes or long-term outcomes. Since imaging is non-invasive, each tumor may serve as its own control making investigations par-ticularly efficient and rigorous. The concept of tumor vascular disruption was proposed over 30 years ago and it remains an active area of research.

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“…seMpai (29b) was developed to improve two weak points in TokeOni: neutral pH and disturbance by hepatic background signals. As TokeOni is acidic [43], it may cause acidosis when injected; moreover, TokeOni detects the hepatic background signals, which are not detected by LH2 [43,44]. Due to the effect of N atom, seMpai was sufficiently soluble for administration to experimental animals and 69 mM was dissolved in phosphate buffered saline (pH 7.4) [40].…”

Section: Bli With Sempaimentioning

confidence: 99%

Near-Infrared Luciferin Analogs for In Vivo Optical Imaging

Saito-Moriya¹,

Obata²,

Maki³

2021

Bioluminescence - Technology and Biology

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The firefly bioluminescence reaction has been exploited for in vivo optical imaging in life sciences. To develop highly sensitive bioluminescence imaging technology, many researchers have synthesized luciferin analogs and luciferase mutants. This chapter first discusses synthetic luciferin analogs and their structure–activity relationships at the luminescence wavelength of the firefly bioluminescence reaction. We then discuss the development of luciferin analogs that produce near-infrared (NIR) light. Since NIR light is highly permeable for biological tissues, NIR luciferin analogs might sensitively detect signals from deep biological tissues such as the brain and lungs. Finally, we introduce two NIR luciferin analogs (TokeOni and seMpai) and a newly developed bioluminescence imaging system (AkaBLI). TokeOni can detect single-cell signals in mouse tissue and luminescence signals from marmoset brain, whereas seMpai can detect breast cancer micro-metastasis. Both reagents are valid for in vivo bioluminescence imaging with high sensitivity.

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High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai

Cited by 22 publications

References 18 publications

How to Select Firefly Luciferin Analogues for In Vivo Imaging

How to Select Firefly Luciferin Analogues for In Vivo Imaging

Non-Invasive Evaluation of Acute Effects of Tubulin Binding Agents: A Review of Imaging Vascular Disruption in Tumors

Near-Infrared Luciferin Analogs for In Vivo Optical Imaging

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