Proliferation of Excised Juice Vesicles of Lemon in vitro

Abstract

all uterine fluid 24 hours before infusion with phenol red did, however, effect bromination.Further studies are in progress to determine the site and mechanism of the bromination. Perhaps there exists, in certain marine animals, an enzyme system which catalyzes the bromination described above. The biological halogenation of phenols may be analogous to the biogenesis of thyroxin where tyrosine residues undergo iodination (8).Abstract. A simple calculation shows that the K ions about to leave the nerve during excitation form a monolayer at the surface of the resting nerve and are not hydrated. The same applies to the Na ions which replace the K ions. This may be taken as an indication of the existence of a reversible ad-and desorption phenomenon.An important ionic relationship associated with the excitation process becomes apparent in the following way. Let it be assumed that there is a specific volume VK of the nerve substance in which the original concentration Ki of potassium ions changes to that of the bathing fluid Ko as a result of the net K ion loss incurred during a single discharge. This takes the form all uterine fluid 24 hours before infusion with phenol red did, however, effect bromination.Further studies are in progress to determine the site and mechanism of the bromination. Perhaps there exists, in certain marine animals, an enzyme system which catalyzes the bromination described above. The biological halogenation of phenols may be analogous to the biogenesis of thyroxin where tyrosine residues undergo iodination (8).

In vitro culture of lemon juice vesicles

“…Initially, callus was observed on the vesicle tip or stalk. These regions corresponded to normal elongation and meristematic regions of the vesicle [4,9]. Callus formation often occurred in areas of vesicle injury (Fig.…”

“…Few sterile fruit culture studies have been performed with either whole organs or isolated tissues [2,6,10,16]. Unfortunately, once isolated tissues (even complex fruit tissues) are introduced into a sterile environment, they often immediately lose structural integrity and dedifferentiate into a rapidly dividing callus mass [9,10,12]. Previous fruit culture studies were concerned with methods to obtain high yields of unorganized callus growth.…”

“…Citrus juice vesicle tissue readily produces callus on a variety of media [2,3,6,8,9,12,15,16,17]. Kordan [9,12] has extensively used mature lemon juice vesicles to study basic plant mitotic activities and callus formation. Meaningful studies using juice vesicle explants in vitro must be conducted in an environment where callusing is minimized or eliminated and normal development occurs.…”

L'Expression de la variabilité dans les cultures d'organes

“…Ainsi, peut-on remarquer, en culture in vitro, que des fragments prélevés dans la fleur ou à son voisinage présentent une meilleure aptitude à la production de cals et (ou (Schroeder, 1955 ;Kordan, 1958Kordan, , 1963Garestier et coll., 1964 ;Nitsch, 1965 ;Tucker, 1967 ;Bouzid, 1977 Ceux-ci traduisent, au moins pour une part, le mode de fonctionnement du matériel héréditaire que la portion prélevée présente du fait de son histoire en tant qu 'élé-ment constitutif d'un ensemble intégré dont l'action de corrélations multiples contribue à forger l'évolution globale au travers de l'évolution de chacune de ses parties.…”

Partial Purification of a Growth Factor from Orange Juice Which Affects Citrus Tissue Culture and Its Replacement by Citric Acid

“…A tissue culture system from citrus albedo (the white, inner portion of citrus peel) has been described by Murashige and Tucker (12), who also reviewed Schroeder's work (16) and other attempts (1,3,8,13,17) to develop tissue cultures from citrus. Murashige and Tucker (12) found that subcultures of citrus albedo explants from species other than C. limon were dependent for their growth on the supply of orange juice to the medium.…”