Proliferation of adherent synovial fluid cell cultures is modified by eicosanoids

Abstract

T. Proliferation of adherent synovial fluid cell cultures is modified by eicosanoids. APMIS 98: 605-608, 1990.Synovial fluid cells obtained from a carragheenan-induced chronic arthritis in the juvenile dog knee were allowed to adhere and proliferate in culture flasks. After twelve days secondary cultures were made and either 10-'M leukotriene B, (LTB,), 25 pM 15-hydroxy-eicosatetraenoic acid (1 SHETE), or both, were added and the cells were cultured for another 6 days. LTB, is generated via the 5-lipoxygenase pathway of arachidonic acid metabolism and stimulates a number of phagocyte functions. Compared to control cells LTB, increased proliferation in 9 out of 10 cell cultures (p < 0.05). The 15-lipoxygenase product, 15-HETE, is not proinflammatory and is an endogenous inhibitor of 5-lipoxygenase. Addition of 15-HETE decreased proliferation of cell cultures by 23% (p < 0.01). It is speculated that LTB, in addition to its effect on phagocytes may play a role in synovial hyperproliferation observed in arthritis.

Effects of ER-34122, a novel dual 5-lipoxygenase/cyclooxygenase inhibitor, on indices of early articular lesion in MRL/MpJ-lpr/lpr mice

“…hibition LOX products generation, as it is reported that LTB 4 increased proliferation of cultured synovial fluid adherent cells and a role of LTB 4 in synovial hyperproliferation has been suggested [18]. The severity of PMN infiltration and subsynovial soft tissue edema declined to the late stage, while cartilage degeneration and pannus formation were severer in the late stage than in the early stage.…”