Proliferation and differentiation potential of cryopreserved human skin‐derived precursors

Abstract

Results revealed that in only 5% DMSO, hSKPs could be cryopreserved for long-term storage with considerable survival and proliferation levels, without losing multipotency.

Differentiation of human skin-derived precursor cells into functional islet-like insulin-producing cell clusters

“…In addition, we described an efficient method for cryopreservation of hSKPs with the aim of establishing master cell banks of younger individuals for putative future use in cell therapy protocols when degenerative diseases arise. This enables the investigator to produce large numbers of hSKPs from a small piece of human foreskin early in life and freeze these cells for later uses (Bakhtiari et al 2012). …”

“…In addition, we have also described an efficient method for cryopreservation of hSKPs with the aim to establish cell banks in future. Preservation and successful long-term storage of hSKPs is an important mandatory step for their potential therapeutic applications in regenerative approaches and autologous cellreplacement therapy (Bakhtiari et al 2012). In the present study, we have generated functional insulin-producing cells (IPCs) from hSKPs using an in vitro differentiation procedure.…”

Germ cell differentiation in cryopreserved, immature, Indian spotted mouse deer (Moschiola indica) testes xenografted onto mice

“…Another possible explanation could be the presence of a high concentration of FBS (i.e., 80%) in the D10S80 cryomedium, which could have aided in effective cryopreservation. Although bovine serum is widely used as a key component of freezing media for cryopreservation of a variety of cell types and tissues, its cryoprotective effects on target cells or tissues are uncertain and sometimes heavily debated [20][21][22]31]. A recent report showed that bovine serum is dispensable for cryopreservation of adult bovine testis tissue [6].…”

“…Fetal bovine serum (FBS), which contains a rich variety of proteins and growth factors, is the most widely used supplement for cell culture that contains a rich variety of proteins and growth factors [19]. Fetal bovine serum is also used as a nonpenetrating cryoprotectant in cryopreservation medium [20][21][22]. However, the effect of DMSO and FBS on Indian mouse deer testis tissues is unknown.…”

Neural Fate of Mesenchymal Stem Cells and Neural Crest Stem Cells: Which Ways to Get Neurons for Cell Therapy Purpose?

“…After adding NGF in the culture medium of human SKPs, Bakthiari et al obtained NF-M, βIII-tubulin, S100 and GFAP positive cells, and that after different conditions of cryopreservation [75].…”