Germ cell differentiation in cryopreserved, immature, Indian spotted mouse deer (Moschiola indica) testes xenografted onto mice

Pothana, Lavanya; Makala, Himesh; Devi, Lalitha; Varma, Vivek Phani; Goel, Sandeep

doi:10.1016/j.theriogenology.2014.10.028

Cited by 25 publications

(28 citation statements)

References 27 publications

(41 reference statements)

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“…2007, (2008); Curaba et al . (2011a); Poels et al . (2013, 2014)UncontrolledMouseImmature1.5 M DMSO + 0.07 M sucroseInsulated container in −80°C, LN2Spz in allograftsGoossens et al .…”

Section: Resultsmentioning

confidence: 99%

“…Nevertheless, the cell viability of immature porcine testicular tissues undergoing the same cryopreservation treatment was not affected by the original size of the tissue fragment (5, 15, 20 or 30 mg) (Abrishami et al ., 2010). Also, for the cryopreservation of tissue from prepubertal boys, varying fragments sizes (2–9 mm³) were successfully frozen (Keros et al ., 2007; Wyns et al ., 2007, 2008; Curaba et al ., 2011a; Poels et al ., 2013, 2014; Picton et al ., 2015). As evidenced by studies using whole ovaries, it would be interesting to see if whole human testes can be cryopreserved (Pfeifer et al ., 2014).…”

Section: Resultsmentioning

confidence: 99%

“…(2014)SheepImmature3 M DMSO + 20% FBSInsulated container in −80°C, LN2Spz after xenograftingPukazhenthi et al . (2015)DeerImmature1.5 M DMSO + 80% FBSInsulated container in −80°C, LN2Spc in xenograftsPothana et al . (2015)BovineMature1.4 M DMSO + 0–20% NCSInsulated container in −80°C, LN2Preservation of SSCsWu et al .…”

Section: Resultsmentioning

confidence: 99%

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Cryopreservation of testicular tissue or testicular cell suspensions: a pivotal step in fertility preservation

Onofre

Baert

Faes

et al. 2016

Hum. Reprod. Update

161

116

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BACKGROUNDGerm cell depletion caused by chemical or physical toxicity, disease or genetic predisposition can occur at any age. Although semen cryopreservation is the first reflex for preserving male fertility, this cannot help out prepubertal boys. Yet, these boys do have spermatogonial stem cells (SSCs) that able to produce sperm at the start of puberty, which allows them to safeguard their fertility through testicular tissue (TT) cryopreservation. SSC transplantation (SSCT), TT grafting and recent advances in in vitro spermatogenesis have opened new possibilities to restore fertility in humans. However, these techniques are still at a research stage and their efficiency depends on the amount of SSCs available for fertility restoration. Therefore, maintaining the number of SSCs is a critical step in human fertility preservation. Standardizing a successful cryopreservation method for TT and testicular cell suspensions (TCSs) is most important before any clinical application of fertility restoration could be successful.OBJECTIVE AND RATIONALEThis review gives an overview of existing cryopreservation protocols used in different animal models and humans. Cell recovery, cell viability, tissue integrity and functional assays are taken into account. Additionally, biosafety and current perspectives in male fertility preservation are discussed.SEARCH METHODSAn extensive PubMED and MEDline database search was conducted. Relevant studies linked to the topic were identified by the search terms: cryopreservation, male fertility preservation, (immature)testicular tissue, testicular cell suspension, spermatogonial stem cell, gonadotoxicity, radiotherapy and chemotherapy.OUTCOMESThe feasibility of fertility restoration techniques using frozen-thawed TT and TCS has been proven in animal models. Efficient protocols for cryopreserving human TT exist and are currently applied in the clinic. For TCSs, the highest post-thaw viability reported after vitrification is 55.6 ± 23.8%. Yet, functional proof of fertility restoration in the human is lacking. In addition, few to no data are available on the safety aspects inherent to offspring generation with gametes derived from frozen-thawed TT or TCSs. Moreover, clarification is needed on whether it is better to cryopreserve TT or TCS.WIDER IMPLICATIONSFertility restoration techniques are very promising and expected to be implemented in the clinic in the near future. However, inter-center variability needs to be overcome and the gametes produced for reproduction purposes need to be subjected to safety studies. With the perspective of a future clinical application, there is a dire need to optimize and standardize cryopreservation and safety testing before using frozen-thawed TT of TCSs for fertility restoration.

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“…2007, (2008); Curaba et al . (2011a); Poels et al . (2013, 2014)UncontrolledMouseImmature1.5 M DMSO + 0.07 M sucroseInsulated container in −80°C, LN2Spz in allograftsGoossens et al .…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Cryopreservation of testicular tissue or testicular cell suspensions: a pivotal step in fertility preservation

Onofre

Baert

Faes

et al. 2016

Hum. Reprod. Update

161

116

View full text Add to dashboard Cite

show abstract

“…Researches involving testicular cryopreservation have made use of xenotransplantation techniques or in vitro cell culture to obtain viable and functional sperm cells for use in assisted reproduction programs after tissue cryopreservation (YOKONISHI et al, 2014;POTHANA et al, 2015). Organ transplantation across species, or xenotransplantation, has been used as a cell culture medium ex situ in different species.…”

Section: Advances and Perspectives In Testicular Cryopreservationmentioning

confidence: 99%

“…After 13 months of culture, fully formed spermatozoa were recovered in 5 of the 29 implanted fragments. POTHANA et al (2015) used cryopreserved testicular fragments of Indian deer (Moschiola indica) for xenograft transplants in immunocompromised mice, and obtained primary spermatocytes after 24 weeks of implantation. ABRISHAMI et al (2010a) grafted cryopreserved testicular tissue from prepubertal pigs into mice.…”

Section: Advances and Perspectives In Testicular Cryopreservationmentioning

confidence: 99%

Cryopreservation of testicular tissue: an alternative to maintain the reproductive capacity in different animal species

Lima

Silva

2017

Cienc. Rural

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Cryopreservation of testicular tissue enables the maintenance of reproductive capacity in different animal species, and contributes to the formation of gene banks for endangered species. The spermatogonia present in the testes can be grown in vitro and the sperm obtained can be used in artificial breeding programs. This review aimed to describe the main techniques of testicular cryopreservation, the main cryoprotectants used, as well as the progress made in different animal species thus far. In the last decade, significant progress has been made in obtaining viable and functional germ cells from testicular tissue. However, more research is needed to better establish protocols that can be used in clinical practice with various species.

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Cryopreservation of Testicular Stem Cells and Its Application in Veterinary Science

Patra

Bhaskar

Gupta

2021

Stem Cells in Veterinary Science

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Germ cell differentiation in cryopreserved, immature, Indian spotted mouse deer (Moschiola indica) testes xenografted onto mice

Cited by 25 publications

References 27 publications

Cryopreservation of testicular tissue or testicular cell suspensions: a pivotal step in fertility preservation

Cryopreservation of testicular tissue or testicular cell suspensions: a pivotal step in fertility preservation

Cryopreservation of testicular tissue: an alternative to maintain the reproductive capacity in different animal species

Cryopreservation of Testicular Stem Cells and Its Application in Veterinary Science

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