Proliferating bacterial symbionts on house fly eggs affect oviposition behaviour of adult flies

Lam, Kevin; Babor, Daron; Duthie, Bruce; Babor, Elisa-Marie; Moore, Margo M.; Gries, Gerhard

doi:10.1016/j.anbehav.2006.11.013

Cited by 82 publications

(69 citation statements)

References 27 publications

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“…While it is not clear to us why this would be the case, the fact is that bacteria release a remarkable number of volatiles (Schulz and Dickschat, 2007) salient to many animals, including humans (Lam et al, 2007;Archie and Theis, 2011;Leroy et al, 2011;Davis et al, 2013). The level of saliency of a volatile, however, is not merely a passive chemical property, because it can also reflect an evolved adaptation by an animal.…”

Section: Microbiome Volatiles As Salient Cuesmentioning

confidence: 86%

Social attraction mediated by fruit flies' microbiome

Venu

Durisko

et al. 2014

Journal of Experimental Biology

103

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Larval and adult fruit flies are attracted to volatiles emanating from food substrates that have been occupied by larvae. We tested whether such volatiles are emitted by the larval gut bacteria by conducting tests under bacteria-free (axenic) conditions. We also tested attraction to two bacteria species, Lactobacillus brevis, which we cultured from larvae in our lab, and L. plantarum, a common constituent of fruit flies' microbiome in other laboratory populations and in wild fruit flies. Neither larvae nor adults showed attraction to axenic food that had been occupied by axenic larvae, but both showed the previously reported attraction to standard food that had been occupied by larvae with an intact microbiome. Larvae also showed significant attraction to volatiles from axenic food and larvae to which we added only either L. brevis or L. plantarum, and volatiles from L. brevis reared on its optimal growth medium. Controlled learning experiments indicated that larvae experienced with both standard and axenic used food do not perceive either as superior, while focal larvae experienced with simulated used food, which contains burrows, perceive it as superior to unused food. Our results suggest that flies rely on microbiome-derived volatiles for longdistance attraction to suitable food patches. Under natural settings, fruits often contain harmful fungi and bacteria, and both L. brevis and L. plantarum produce compounds that suppress the growth of some antagonistic fungi and bacteria. The larval microbiome volatiles may therefore lead prospective fruit flies towards substrates with a hospitable microbial environment.

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Section: Microbiome Volatiles As Salient Cuesmentioning

confidence: 86%

Social attraction mediated by fruit flies' microbiome

Venu

Durisko

et al. 2014

Journal of Experimental Biology

103

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“…Other bacteria identified from collected flies in this study, including Proteus spp., Proteus vulgaris, Proteus hauseri, Proteus penneri, Klebsiella pneumoniae, Providencia alcalifaciens, Providencia stuartii, Enterobacter spp., Enterobacter cloacae, Enterobacter cancerogenus, and Citrobacter freundii (see Table S1 in the supplemental material), have also been isolated from flies in other stud-ies (10,19,35,54,83). These microorganisms are considered indigenous microbiota in flies as they can establish symbiotic relationships with the host, providing nutrients or other defensive compounds (56).…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Relative Risk of Cronobacter spp., Salmonella spp., and Listeria monocytogenes Associated with the Body Surfaces and Guts of Individual Filth Flies

Pava‐Ripoll

Pearson

Miller

et al. 2012

Appl Environ Microbiol

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Although flies are important vectors of food-borne pathogens, there is little information to accurately assess the food-related health risk of the presence of individual flies, especially in urban areas. This study quantifies the prevalence and the relative risk of food-borne pathogens associated with the body surfaces and guts of individual wild flies. One hundred flies were collected from the dumpsters of 10 randomly selected urban restaurants. Flies were identified using taxonomic keys before being individually dissected. Cronobacter spp., Salmonella spp., and Listeria monocytogenes were detected using the PCR-based BAX system Q7. Positive samples were confirmed by culture on specific media and through PCR amplification and sequencing or ribotyping. Among collected flies were the housefly, Musca domestica (47%), the blowflies, Lucilia cuprina (33%) and Lucilia sericata (14%), and others (6%). Cronobacter species were detected in 14% of flies, including C. sakazakii, C. turicensis, and C. universalis, leading to the proposal of flies as a natural reservoir of this food-borne pathogen. Six percent of flies carried Salmonella enterica, including the serovars Poona, Hadar, Schwarzengrund, Senftenberg, and Brackenridge. L. monocytogenes was detected in 3% of flies. Overall, the prevalence of food-borne pathogens was three times greater in the guts than on the body surfaces of the flies. The relative risk of flies carrying any of the three pathogens was associated with the type of pathogen, the body part of the fly, and the ambient temperature. These data enhance the ability to predict the microbiological risk associated with the presence of individual flies in food and food facilities.

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“…Microbes can also influence attraction of insects to their hosts (Hilker and Meiners 2002). For instance, Proteus mirabilis attracts L. sericata (Ma et al 2012b;Tomberlin et al 2012), Musca domestica Linnaeus (Diptera: Muscidae) females have been shown to prefer to oviposit on eggs coated with certain Gram-positive bacteria (Lam et al 2007), and Aedes aegypti (Linnaeus) (Diptera: Culicidae) prefer oviposition on a mixture of 14 bacteria isolates from bamboo leaf infusion compared to water as a control (Ponnusamy et al 2008). Since bacteria and their associated metabolites can influence blow fly behavior, it seems likely that bacterial research with these flies will have repercussions for forensic, medical, veterinary, and agricultural applications (Tomberlin et al 2012).…”

Section: Introductionmentioning

confidence: 99%

A metagenomic assessment of the bacteria associated with Lucilia sericata and Lucilia cuprina (Diptera: Calliphoridae)

Singh

Crippen

Zheng

et al. 2014

Appl Microbiol Biotechnol

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Lucilia Robineau-Desvoidy (Diptera: Calliphoridae) is a blow fly genus of forensic, medical, veterinary, and agricultural importance. This genus is also famous because of its beneficial uses in maggot debridement therapy (MDT). Although the genus is of considerable economic importance, our knowledge about microbes associated with these flies and how these bacteria are horizontally and trans-generationally transmitted is limited. In this study, we characterized bacteria associated with different life stages of Lucilia sericata (Meigen) and Lucilia cuprina (Wiedemann) and in the salivary gland of L. sericata by using 16S rDNA 454 pyrosequencing. Bacteria associated with the salivary gland of L. sericata were also characterized using light and transmission electron microscopy (TEM). Results from this study suggest that the majority of bacteria associated with these flies belong to phyla Proteobacteria, Firmicutes, and Bacteroidetes, and most bacteria are maintained intragenerationally, with a considerable degree of turnover from generation to generation. In both species, second-generation eggs exhibited the highest bacterial phylum diversity (20 % genetic distance) than other life stages. The Lucilia sister species shared the majority of their classified genera. Of the shared bacterial genera, Providencia, Ignatzschineria, Lactobacillus, Lactococcus, Vagococcus, Morganella, and Myroides were present at relatively high abundances. Lactobacillus, Proteus, Diaphorobacter, and Morganella were the dominant bacterial genera associated with a survey of the salivary gland of L. sericata. TEM analysis showed a sparse distribution of both Gram-positive and Gram-negative bacteria in the salivary gland of L. sericata. There was more evidence for horizontal transmission of bacteria than there was for trans-generational inheritance. Several pathogenic genera were either amplified or reduced by the larval feeding on decomposing liver as a resource. Overall, this study provides information on bacterial communities associated with different life stages of Lucilia and their horizontal and trans-generational transmission, which may help in the development of better vector-borne disease management and MDT methods.

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Proliferating bacterial symbionts on house fly eggs affect oviposition behaviour of adult flies

Cited by 82 publications

References 27 publications

Social attraction mediated by fruit flies' microbiome

Social attraction mediated by fruit flies' microbiome

Prevalence and Relative Risk of Cronobacter spp., Salmonella spp., and Listeria monocytogenes Associated with the Body Surfaces and Guts of Individual Filth Flies

A metagenomic assessment of the bacteria associated with Lucilia sericata and Lucilia cuprina (Diptera: Calliphoridae)

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