Prolactine Receptor Expression in Kidney Tissue of Female Rats with Cholestasis: the Effect of Hyperprolactinemia

Abstract

Immunohistochemistry with semiquantitative image analysis showed that prolactin receptor in distal renal tubules of female rats is most sensitive to the negative effects of both cholestasis and hyperprolactinemia. The responses of medullary tubules to cholestasis and hyperprolactinemia were less pronounced: decrease and increase in prolactin receptor expression, respectively. Proximal tubules were characterized by stable levels of prolactin receptor expression insensitive to the effects of obstructive cholestasis and hyperprolactinemia. The cholestasis-induced changes in the intensity of prolactin receptor expression were opposite in kidney and liver cells. It is concluded that different parts of the nephron differ by the presence, type, and direction of regulation of prolactin receptor expression in obstructive cholestasis and hyperprolactinemia.

Expression of Multidrug Resistance Protein 2 (mrp2) in the Liver and Kidney Cells of Female Rats with Modeled Cholestasis of Pregnancy

“…In previous studies, we revealed opposite changes in prolactin receptors expression in the liver and kidney cells in cholestasis and changes in mrp3 and CFTR transporter expression in these cells in modeled cholestasis of pregnancy [1][2][3]. The obtained results attest to shifts of prolactin targets in cholestasis of pregnancy.…”

“…Expression of mrp2 was compared with prolactin receptor [1] in liver and kidney cells. In bile ducts cells, a negative correlation between mrp2 and prolactin receptor was revealed (Fig.…”

“…Hyperprolactinemia was simulated using a routine method of donor pituitary transplantation under the renal capsule of a recipient [1,2]. Obstructive cholestasis was induced by ligation of the common bile duct for 2 weeks.…”

“…Expression intensity was evaluated using semiquantitative analysis of images of these structures (50 cells or parts of each type of the tubule for each animal) obtained with Canon PowerShot G10 photosystem linked with a microscope (BIOMED) by light transmission using ImageJ 1.40 software after raw to tiff conversion. Expression was evaluated by the difference between staining intensity (proportional to relative concentration of the label) in experimental and control sections in blue spectrum (parameter range from 0 to 65536) [1,2] The data were processed using Statistica 6.0 software (StatSoft Inc.). The groups were tested for paired group differences.…”

“…The differences are signifi cant at p<0.05 (nonparametric Mann-Whitney test). Spear- man's correlation coeffi cient and statistical significance of correlations between mrp2 and previously studied prolactin receptor [1], mrp3 [2], and CFTR [3] were calculated for each cell type considering all studied groups.…”