Prolactin prevents mitochondrial dysfunction induced by glutamate excitotoxicity in hippocampal neurons

Rivero-Segura, Nadia Alejandra; Coronado-Mares, Isabel; Rincón-Heredia, Ruth; Pérez-Torres, Israel; Montiel, Teresa; Pavón, Natalia; Cabrera-Reyes, Erika Alejandra; Massieu, Lourdes; Cerbón, Marco

doi:10.1016/j.neulet.2019.02.027

Cited by 34 publications

(14 citation statements)

References 33 publications

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“…It is widely established that mitochondria play a vital role in the development of neurons at the subcellular level [ 36 ]. As a result, the current investigation of the mitochondrial basis of EAV-400's anti-convulsant action against STR-induced convulsion in experimental rats.…”

Section: Discussionmentioning

confidence: 99%

Attenuation of Strychnine-Induced Epilepsy Employing Amaranthus viridis L. Leaves Extract in Experimental Rats

Bharadwaj

Sharma

Singh

et al. 2023

Behavioural Neurology

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Objective. Epilepsy is one of the most prevalent neurological illnesses defined by periodic seizures with or without loss of consciousness caused by aberrant neural activity. There are many allopathic medications available for the treatment of epilepsy such as phenytoin (PHY), but the side effects are a major concern. Therefore, the present study involved the evaluation of the pharmacological significance of Amaranthus viridis L. extract (EAV) in the management of strychnine (STR)-induced epilepsy. Method. STR (3.5 mg/kg, i.p.) was injected into male rats 30 minutes after the pre-treatment of a standard drug (PHY: 20 mg/kg) and the two doses of EAV (EAV-200 and EAV-400 mg/kg, p.o.) to the respective groups to cause the convulsions. The anti-convulsant effect of EAV-200 and EAV-400 against STR-induced convulsion in rats was investigated in terms of convulsion onset, duration of convulsions, number of convulsions, and convulsion score. Furthermore, the mitochondrial function and integrity in the brain’s prefrontal cortex (PFC) were also estimated. Results. EAV-400 significantly increased the onset of convulsion from 61.67 ± 3.051 to 119.2 ± 2.738 and reduced the STR-induced duration of convulsions from 144.8 ± 3.582 to 69.17 ± 3.736 , number of convulsions from 4.000 ± 0.1592 to 1.533 ± 0.1542 , and convulsion score from 5.000 ± 0.3651 to 2.833 ± 0.3073 in rats. EAV-400 significantly attenuated the STR-induced decrease in the mitochondrial function and integrity of the rat PFC. In rats, EAV-400 significantly accelerated the onset of convulsions while decreasing the STR-induced duration, frequency, and score. Conclusion. Based on investigational findings, EAV-400 could be inferred to be a possible anti-epileptic option for the treatment of epilepsy of this plan in preclinical research.

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Section: Discussionmentioning

confidence: 99%

Attenuation of Strychnine-Induced Epilepsy Employing Amaranthus viridis L. Leaves Extract in Experimental Rats

Bharadwaj

Sharma

Singh

et al. 2023

Behavioural Neurology

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“…Protein concentrations were determined by BCA assay following the supplier’s instructions (cat# 23227, Thermo Fisher Scientific, Waltham, MA, USA). Then immunoblots were performed following the protocol reported in Rivero-Segura et al [ 50 ]. Briefly, protein samples were re-suspended in loading buffer (cat#1610747, BioRad, Hercules, CA, USA) supplemented with 5% β-mercaptoethanol and denatured at 95 °C for 5 min in a block heater.…”

Section: Methodsmentioning

confidence: 99%

Insomnia Impairs Both the Pro-BDNF and the BDNF Levels Similarly to Older Adults with Cognitive Decline: An Exploratory Study

Sánchez-García

Moreno-Tamayo

Ramírez‐Aldana

et al. 2023

IJMS

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Sleep disorders, including insomnia, are common during aging, and these conditions have been associated with cognitive decline in older adults. Moreover, during the aging process, neurotransmitters, neurohormones, and neurotrophins decrease significantly, leading to the impairment of cognitive functions. In this sense, BDNF, the most abundant neurotrophic factor in the human brain, has been suggested as a potential target for the prevention and improvement of cognitive decline during aging; however, the current evidence demonstrates that the exogenous administration of BDNF does not improve cognitive function. Hence, in the present study, we quantified pro-BDNF (inactive) and BDNF (active) concentrations in serum samples derived from older individuals with insomnia and/or cognitive decline. We used linear regression to analyze whether clinical or sociodemographic variables impacted the levels of BNDF concentration. We observed that insomnia, rather than cognitive decline, is significantly associated with BDNF concentration, and these effects are independent of other variables. To our knowledge, this is the first study that points to the impact of insomnia on improving the levels of BDNF during aging and suggests that opportune treatment of insomnia may be more beneficial to prevent cognitive decline during aging.

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“…After 6 days in coculture, corresponding to neurons 12 DIV, the mitochondrial membrane potential was assayed by using the cationic colorant JC1 (Thermo Fisher Scientific, MA, United States) ( Rivero-Segura et al, 2019 ). For this assay, the newly isolated neurons were cultured in coverslips and laid inside the bottom of the transwell at day 0 and the normal coculture procedure was performed.…”

Section: Methodsmentioning

confidence: 99%

Senescence in Primary Rat Astrocytes Induces Loss of the Mitochondrial Membrane Potential and Alters Mitochondrial Dynamics in Cortical Neurons

Morales-Rosales

Santín-Márquez

Posadas-Rodríguez

et al. 2021

Front. Aging Neurosci.

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The decline in brain function during aging is one of the most critical health problems nowadays. Although senescent astrocytes have been found in old-age brains and neurodegenerative diseases, their impact on the function of other cerebral cell types is unknown. The aim of this study was to evaluate the effect of senescent astrocytes on the mitochondrial function of a neuron. In order to evaluate neuronal susceptibility to a long and constant senescence-associated secretory phenotype (SASP) exposure, we developed a model by using cellular cocultures in transwell plates. Rat primary cortical astrocytes were seeded in transwell inserts and induced to premature senescence with hydrogen peroxide [stress-induced premature senescence (SIPS)]. Independently, primary rat cortical neurons were seeded at the bottom of transwells. After neuronal 6 days in vitro (DIV), the inserts with SIPS-astrocytes were placed in the chamber and cocultured with neurons for 6 more days. The neuronal viability, the redox state [reduced glutathione/oxidized glutathione (GSH/GSSG)], the mitochondrial morphology, and the proteins and membrane potential were determined. Our results showed that the neuronal mitochondria functionality was altered after being cocultured with senescent astrocytes. In vivo, we found that old animals had diminished mitochondrial oxidative phosphorylation (OXPHOS) proteins, redox state, and senescence markers as compared to young rats, suggesting effects of the senescent astrocytes similar to the ones we observed in vitro. Overall, these results indicate that the microenvironment generated by senescent astrocytes can affect neuronal mitochondria and physiology.

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Prolactin prevents mitochondrial dysfunction induced by glutamate excitotoxicity in hippocampal neurons

Cited by 34 publications

References 33 publications

Attenuation of Strychnine-Induced Epilepsy Employing Amaranthus viridis L. Leaves Extract in Experimental Rats

Attenuation of Strychnine-Induced Epilepsy Employing Amaranthus viridis L. Leaves Extract in Experimental Rats

Insomnia Impairs Both the Pro-BDNF and the BDNF Levels Similarly to Older Adults with Cognitive Decline: An Exploratory Study

Senescence in Primary Rat Astrocytes Induces Loss of the Mitochondrial Membrane Potential and Alters Mitochondrial Dynamics in Cortical Neurons

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