Proinflammatory and immunoregulatory mechanisms in periapical lesions

Čolić, Miodrag; Gazivoda, Dragan; Vučević, Dragana; Vasilijić, Saša; Rudolf, Rebeka; Lukić, Aleksandra

doi:10.1016/j.molimm.2009.01.011

Cited by 128 publications

(138 citation statements)

References 82 publications

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“…We used a model of PL-MSCs co-culture with infiltrating PL cells. These cells, as shown in this study and our previous paper (16), are composed of lymphocytes and B-cell effectors (plasma cells) together with innate immune cells (neutrophil granulocytes and macrophages). Therefore, this model reflects better the function of PL-MSCs in vivo compared to the models using peripheral blood mononuclear cells.…”

Section: Discussionsupporting

confidence: 72%

“…At the moment we do not know how PL-MSCs up-regulate IL-10 secretion by PL-ICs, since the complex pathways between different proinflammatory and anti-inflammatory cytokines exist in PL and other chronic inflammatory diseases (16). The best examined pathway includes the balance between Th17 cells and Tregs (18).…”

Section: O R I G I N a L A R T I C L Ementioning

confidence: 99%

“…Although TGF-β stimulates initially the inflammatory responses by attracting lymphocytes and monocytes to the inflammatory site, it suppresses the proliferation of these cells as well as the production of different proinflammatory cytokines (19). TGF-β is also expressed in PLs (16,20), irrespective of whether PLs are asymptomatic or symptomatic. It can be postulated that this cytokine is important for down-regulation of inflammatory and immune responses within PLs and for the repair of periapical bone loss.…”

Section: O R I G I N a L A R T I C L Ementioning

confidence: 99%

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Mesenchymal Stem Cells from Periapical Lesions Upregulate the Production of Immunoregulatory Cytokines by Inflammatory Cells in Culture / Mezenhimske matične ćelije iz periapeksnih lezija stimulišu produkciju imunoregulacijskih citokina od strane inflamacijskih ćelija u kulturi

Marković¹,

Tomić²,

Djokić³

et al. 2015

Acta Facultatis Medicae Naissensis

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The pathophysiology of periapical lesions (PLs) is under control of pro-inflammatory and anti-inflammatory (mainly immunoregulatory) cytokines. We have recently established mesenchymal stem cells (MSCs) from PLs and showed their suppressive effects on the production of proinflammatory cytokines from PLs inflammatory cells (ICs). In this work we studied the production of interleukin (IL)-10, IL-27 and transforming growth factor (TGF)-β, by PL-ICs in direct or indirect contacts with PL-MSCs. PL-ICs, which were isolated from four different asymptomatic PLs, predominantly composed of lymphocytes, followed by neutrophil granulocytes, macrophages and plasma cells. PLMSCs, expressing typical MSC markers, were co-cultivated with PL-ICs at 1:10 ratio, either in direct contact or in a transwell-system, for 24 hours. The levels of cytokines in cell-culture supernatants were tested by ELISA. The results showed that PL-MSCs up-regulated the production of all three immunoregulatory cytokines by PL-ICs. PL-MSCs stimulated the production of IL-10 and IL-27 via soluble factors, whereas the up-regulation of TGF-β required direct cell-to-cell contacts. In conclusion, our results showed for the first time the involvement of PL-MSCs in restriction of inflammation in PLs by up-regulation of immunoregulatory cytokines.

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Section: Discussionsupporting

confidence: 72%

Section: O R I G I N a L A R T I C L Ementioning

confidence: 99%

Section: O R I G I N a L A R T I C L Ementioning

confidence: 99%

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Mesenchymal Stem Cells from Periapical Lesions Upregulate the Production of Immunoregulatory Cytokines by Inflammatory Cells in Culture / Mezenhimske matične ćelije iz periapeksnih lezija stimulišu produkciju imunoregulacijskih citokina od strane inflamacijskih ćelija u kulturi

Marković¹,

Tomić²,

Djokić³

et al. 2015

Acta Facultatis Medicae Naissensis

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“…In phase 2, the full-text of 18 studies were retrieved and evaluated. Of those, only eight 14,15,16,17,18,19,20,21 met the eligibility criteria. No additional study was identified in the reference lists of the included articles and in the grey literature.…”

Section: Study Selectionmentioning

confidence: 99%

“…14,15,18,21 All studies involved human periapical lesion fragments. Samples of six articles 14,15,18,19,20,21 were obtained directly through surgery and samples of the other two studies were obtained through institutional archives collected from previous surgical procedures. 16,17 Fou r a r t icles repor ted t he mea n age of participants 18,19,20,21 and five studies defined the range between 17 and 64 years.…”

Section: Study Characteristicsmentioning

confidence: 99%

Treg and Th17 cells in inflammatory periapical disease: a systematic review

et al. 2017

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The process involved in periapical lesions, which occur as an outcome of pulpal necrosis, is regulated by the immune system including regulatory T cells (Treg) and T helper 17 cell (Th17) responses. The objective of this study was to conduct a frequency systematic review to determine the presence of Treg/Th17 responses and the influence of these cells in the progression of chronic inflammatory periapical lesions in humans. A systematic computerized search was carried out in Pubmed, Medline, Web of Science and Scopus electronic databases from their date of inception through the first week of May 2017. In addition, the reference lists of the included articles and the grey literature were hand-searched. Articles that evaluated the presence and influence of Treg/Th17 in the progression of human periapical lesions were included. Study selection and the quality assessment of the included articles (using the Newcastle-Ottawa scale) were carried out by two authors. Fifty-seven titles/abstracts were screened and eight studies met the eligibility criteria and were included in this systematic review. The included studies showed large variation in the type of periapical lesion assessed, mean age, age range, type of experiment and findings regarding the participation of Th17 and Treg in the status of inflammatory periapical lesions. The studies showed the involvement of Treg in the modulation of the inflammatory response in radicular cysts and periapical granulomas. This systematic review highlights the relationship between Treg and Th17 acting in a subtle balance inhibiting or promoting the progression of human periapical lesions.

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Healing of Apical Lesions: How Do They Heal, Why Does the Healing Take So Long, and Why Do Some Lesions Fail to Heal?

Metzger

Kfir

2014

Disinfection of Root Canal Systems

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Proinflammatory and immunoregulatory mechanisms in periapical lesions

Cited by 128 publications

References 82 publications

Mesenchymal Stem Cells from Periapical Lesions Upregulate the Production of Immunoregulatory Cytokines by Inflammatory Cells in Culture / Mezenhimske matične ćelije iz periapeksnih lezija stimulišu produkciju imunoregulacijskih citokina od strane inflamacijskih ćelija u kulturi

Mesenchymal Stem Cells from Periapical Lesions Upregulate the Production of Immunoregulatory Cytokines by Inflammatory Cells in Culture / Mezenhimske matične ćelije iz periapeksnih lezija stimulišu produkciju imunoregulacijskih citokina od strane inflamacijskih ćelija u kulturi

Treg and Th17 cells in inflammatory periapical disease: a systematic review

Healing of Apical Lesions: How Do They Heal, Why Does the Healing Take So Long, and Why Do Some Lesions Fail to Heal?

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