Progress with Nonhuman Primate Embryonic Stem Cells1

Wolf, Don P.; Kuo, Hung-Chih; Pau, K.-Y. Francis; Lester, Linda B.

doi:10.1095/biolreprod.104.029413

Cited by 47 publications

(36 citation statements)

References 38 publications

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“…Among them, TRA-1-81 antigen is a keratan sulfate proteoglycan [6,8], which is expressed in undifferentiated cells and dramatically downregulated in differentiated cells. It was suggested that TRA-1-81 and TRA-1-60 may react with the same protein which has sialidase-sensitive epitopes [9].…”

Section: Introductionmentioning

confidence: 99%

Profiling TRA-1-81 antigen distribution on a human embryonic stem cell

Qiu

Xiang

et al. 2008

Biochemical and Biophysical Research Communications

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Human embryonic stem (hES) cells hold great promise in regenerative medicine. Although hES cells have unlimited self-renewal potential, they tend to differentiate spontaneously in culture. TRA-1-81 is a biomarker of undifferentiated hES cells. Quantitative characterization of TRA-1-81 expression level in a single cell helps capture the "turn-on" signal and understand the mechanism of early differentiation. Here we report on our examination of TRA-1-81 distribution and association on a hES cell membrane using an atomic force microscope (AFM). Our results suggest that aggregated distribution of TRA-1-81 antigen is characteristic for undifferentiated hES cells. We also evaluated the TRA-1-81 expression level at ~17800 epitopes and ~700 epitopes per cell on an undifferentiated cell and a spontaneously differentiated cell, respectively. The method in this study can be adapted in examining other surface proteins on various cell types, thus providing a general tool for investigating protein distribution and association at the single cell level.

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Section: Introductionmentioning

confidence: 99%

Profiling TRA-1-81 antigen distribution on a human embryonic stem cell

Qiu

Xiang

et al. 2008

Biochemical and Biophysical Research Communications

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“…The in vitro induction of neural differentiation in ESC also closely mimics molecular mechanisms of embryonic brain development [33]. All these findings make it possible to use rESC as a model for investigating cell lineage relations to better understand primate, including human, embryonic neurogenesis and gliogenesis because the rhesus monkey has the advantage over rodents of being closely related to humans in terms of genetics and physiology [34].…”

Section: Introductionmentioning

confidence: 88%

Neural lineage development in the rhesus monkey with embryonic stem cells

Chen¹,

Wei²,

Zhang³

et al. 2013

Translational Neuroscience

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There are three controversial and undetermined models of neurogenesis and gliogenesis from neuroepithelial cells in the early neural tube; the first in which neurons and glia were proposed to originate from a single homogenous population, the second from two separate pools of committed glial and neuronal progenitors, or, lastly, from transit radial glial (RG). Issues concerning embryonic neural lineage development in primates are not well understood due to restrictions imposed by ethics and material sources. In this study, early neural lineage development was investigated in vitro with rhesus monkey embryonic stem cells (rESC) by means of immunofluorescence with lineage specific markers. It was revealed that neural differentiation likely progresses in a sequential lineage restriction pathway from neuroepithelial stem/progenitor cells to neurons and glia via RG and intermediate precursors: neuronal precursors and glial progenitors. In conclusion, our results suggest that the early neural lineage development of rESC in vitro supported the model in which neuroepithelial cells develop into RG capable of generating both neurons and glia. This work should facilitate understanding of the mechanism of development of the nervous system in primates.

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“…Some feeder-free systems support the culture of undifferentiated hES cells more efficiently than others [8,9], however, porcine and bovine epiblast and ICM cells cultured on a feeder free system did not grow continuously and started senescence or differentiation [10][11][12][13]. In case of the farm animals, ES cell-like cells are also commonly cultured on feeder cells because the molecular pathways and key molecules required in maintaining pluripotency in these species are still unknown [14]. As stated earlier in various reports, homologous feeder cell layers supported self renewal and maintained pluripotent characteristics of human and monkey ES cells, with characteristics similar to those of ES cells cultured on mouse fetal fibroblast (MEF) [9,15].…”

Section: Introductionmentioning

confidence: 99%

Derivation of buffalo embryonic stem-like cells from in vitro-produced blastocysts on homologous and heterologous feeder cells

Kumar

Anand

Singh

et al. 2011

J Assist Reprod Genet

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Purpose The aim of the present study is to compare the ability of homologous and heterologous embryonic fibroblast feeder layers to support isolation and proliferation of buffalo ES-like cells generated from hatched and expanded blastocysts produced by in vitro fertilization and characterization of derived cells through expression of pluripotent markers. Methods Embryonic stem cells were derived from hatched and expanded blastocysts through intact blastocyst culture and enzymatic method respectively and compared for proliferation rate on homologous (buffalo) and heterologous feeder layers (goat and sheep). Results A total of 69 hatched and 83 expanded blastocysts were used for isolation of inner cell masses which were seeded on buffalo, goat and sheep embryonic feeder layers. Following seeding, attachment rate, primary colony formation rate and survival to maximum number of passages were observed to be higher on homologous feeder layers.Conclusions Upon comparison of different feeder layer cells for derivation and maintenance of buffalo ES-like cells from hatched and expanded blastocysts, buffalo embryonic fibroblast cells were able to provide a better environment for maintaining pluripotency in culture conditions.

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Progress with Nonhuman Primate Embryonic Stem Cells1

Cited by 47 publications

References 38 publications

Profiling TRA-1-81 antigen distribution on a human embryonic stem cell

Profiling TRA-1-81 antigen distribution on a human embryonic stem cell

Neural lineage development in the rhesus monkey with embryonic stem cells

Derivation of buffalo embryonic stem-like cells from in vitro-produced blastocysts on homologous and heterologous feeder cells

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