Derivation of buffalo embryonic stem-like cells from in vitro-produced blastocysts on homologous and heterologous feeder cells

Kumar, Dharmendra; Anand, Taruna; Singh, Kiran; Singh, Manoj Kumar; Shah, Riaz Ahmad; Chauhan, M. S.; Palta, P.; Singla, S. K.; Manik, R. S.

doi:10.1007/s10815-011-9572-2

Cited by 16 publications

(4 citation statements)

References 45 publications

(57 reference statements)

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“…Whereas several earlier studies have indicated the requirement of LIF for the maintenance of buffalo ES celllike cells in an undifferentiated state, the presence of LIF alone is not sufficient for their long-term culture (Verma et al 2007;Anand et al 2011;Kumar et al 2011). We have recently shown that a combination of LIF and bFGF is capable of supporting long-term self-renewal of buffalo ES cell-like cells on BFF feeder-layer support (Sharma et al 2011), indicating that both these signalling pathways are operative in buffalo ES cell-like cells.…”

Section: Discussionmentioning

confidence: 98%

Growth factor expression pattern of homologous feeder layer for culturing buffalo embryonic stem cell-like cells

Sharma

George

Kamble

et al. 2012

Reprod. Fertil. Dev.

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The present study examined the expression profile of buffalo fetal fibroblasts (BFF) used as a feeder layer for embryonic stem (ES) cell-like cells. The expression of important growth factors was detected in cells at different passages. Mitomycin-C inactivation increased relative expression levels of ACTIVIN-A, TGF-β1, BMP-4 and GREMLIN but not of fibroblast growth factor-2 (FGF-2). The expression level of ACTIVIN-A, transforming growth factor-β1 (TGF-β1), bone morphogenetic protein-4 (BMP-4) and FGF-2 was similar in buffalo fetal fibroblast (BFF) cultured in stem cell medium (SCM), SCM+1000IU mL(-1) leukemia inhibitory factor (LIF), SCM+5 ngmL(-1) FGF-2 or SCM+LIF+FGF-2 for 24 h whereas GREMLIN expression was higher in FGF-2-supplemented groups. In spent medium, the concentration of ACTIVIN-A was higher in FGF-2-supplemented groups whereas that of TGF-β1 was similar in SCM and LIF+FGF-2, which was higher than when either LIF or FGF-2 was used alone. Following culture of ES cell-like cells on a feeder layer for 24 h, the TGF-β1 concentration was higher with LIF+FGF-2 than with LIF or FGF-2 alone which, in turn, was higher than that in SCM. In the LIF+FGF-2 group, the concentration of TGF-β1 was lower and that of ACTIVIN-A was higher in spent medium at 24 h than at 48 h of culture. These results suggest that BFF produce signalling molecules that may help in self-renewal of buffalo ES cell-like cells.

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Section: Discussionmentioning

confidence: 98%

Growth factor expression pattern of homologous feeder layer for culturing buffalo embryonic stem cell-like cells

Sharma

George

Kamble

et al. 2012

Reprod. Fertil. Dev.

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“…Studies deriving iPSCs from other species have shown the process is applicable across a wide range of animals. For example, multiple groups have derived iPSCs from dogs, horses, sheep, pigs, goats and other wild species [11][12][13][14][15][16][17][18]. With respect to felids, there are two studies describing the generation of iPSCs from wild species [19,20].…”

Section: Introductionmentioning

confidence: 99%

Inducing Pluripotency in the Domestic Cat (Felis catus)

Dutton

Dudhia

Guest

et al. 2019

Stem Cells and Development

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Domestic cats suffer from a range of inherited genetic diseases, many of which display similarities with equivalent human conditions. Developing cellular models for these inherited diseases would enable drug discovery, benefiting feline health and welfare as well as enhancing the potential of cats as relevant animal models for translation to human medicine. Advances in our understanding of these diseases at the cellular level have come from the use of induced pluripotent stem cells (iPSCs). iPSCs are capable of differentiating into derivatives of all three germ layers, therefore overcoming the limitations of primary differentiated cells and the ethical concerns of using embryonic stem cells. No studies however report the generation of iPSCs from domestic cats (fiPSCs). Feline adipose derived fibroblasts were infected with amphotropic retrovirus containing the coding sequences for human Oct4, Sox2, Klf4, cMyc and Nanog. Isolated iPSC clones were expanded on mouse inactivated embryonic fibroblasts in the presence of feline leukaemia inhibitory factor (LIF). Retroviral delivery of human pluripotent genes gave rise to putative fiPSC colonies within 5-7 days. These iPS-like cells required foetal bovine serum and feline LIF for maintenance. Colonies were domed with refractile edges, similar to mouse iPSCs. Immunocytochemistry demonstrated positive staining for stem cell markers: alkaline phosphatase, Oct4, Sox2, Nanog and SSEA1. Cells were negative for SSEA4. Expression of endogenous feline Nanog was confirmed by qPCR. The cells were able to differentiate in vitro into cells representative of the three germ layers. These results confirm the generation of the first induced pluripotent cells from domestic cats. These cells will provide valuable models to study genetic diseases and explore novel therapeutic strategies.

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“…The ES-like cells have shown expression of some pluripotency markers, stem cell-specific cell surface antigens and ability of cells to differentiate into other cell types in buffaloes (Verma et al 2007;Anand et al 2011;Kumar et al 2011 have shown expression of key pluripotency genes in the AFderived stem cell-like cells in buffalo. In the present study, the amnion-derived stem cells show comparable expression of key pluripotency genes, namely, NANOG, OCT-4 and SOX-2.…”

Section: Discussionmentioning

confidence: 99%

Culture, characterization and differentiation of cells from buffalo (Bubalus bubalis) amnion

et al. 2012

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Stem cells present an important tool in livestock assisted reproduction and veterinary therapeutic field such as tissue engineering. We report for the first time isolation of pluripotent stem cell-like cells expressing pluripotency markers (alkaline phospahatase, OCT-4, NANOG and SOX-2) from the amnion of water buffalo (Bubalus bubalis). The cells showed no apparent abnormalities in their chromosomal profiles before and after cryopreservation. The cytochemical staining revealed that pluripotent cells were capable of undergoing directed differentiation in vitro into osteocytes. It could be inferred that amnionderived pluripotent stem cell-like cells can be isolated, cultured for many passages and differentiated into mesoderm lineage, and may be an alternative source to mesenchymal stem cells. These cells can have applications in assisted reproduction, developmental biological and regenerative medicine.

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Derivation of buffalo embryonic stem-like cells from in vitro-produced blastocysts on homologous and heterologous feeder cells

Cited by 16 publications

References 45 publications

Growth factor expression pattern of homologous feeder layer for culturing buffalo embryonic stem cell-like cells

Growth factor expression pattern of homologous feeder layer for culturing buffalo embryonic stem cell-like cells

Inducing Pluripotency in the Domestic Cat (Felis catus)

Culture, characterization and differentiation of cells from buffalo (Bubalus bubalis) amnion

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