Stem cells present an important tool in livestock assisted reproduction and veterinary therapeutic field such as tissue engineering. We report for the first time isolation of pluripotent stem cell-like cells expressing pluripotency markers (alkaline phospahatase, OCT-4, NANOG and SOX-2) from the amnion of water buffalo (Bubalus bubalis). The cells showed no apparent abnormalities in their chromosomal profiles before and after cryopreservation. The cytochemical staining revealed that pluripotent cells were capable of undergoing directed differentiation in vitro into osteocytes. It could be inferred that amnionderived pluripotent stem cell-like cells can be isolated, cultured for many passages and differentiated into mesoderm lineage, and may be an alternative source to mesenchymal stem cells. These cells can have applications in assisted reproduction, developmental biological and regenerative medicine.
The Antinuclear antibodies (ANA) and dsDNA are commonly used as a screening test for various suspected connective tissue disorders and autoimmune diseases. They help to monitor disease activity along with diagnostic and prognostic assessment. Systemic lupus erythematosus . (SLE) is one of the main immune complex mediated disease. Antibodies to double-stranded deoxyribonucleic acid (dsDNA) are most sensitive for diagnosis of systemic lupus erythematosus. Infectious and environmental factors which, mainly include seasonal variation, interact for the autoimmune disease development. The seasonal variation found in ANA and dsDNA tend to increase during the colder months. Anti dsDNA in combination with the levels of complement proteins C3 and C4, fluctuate with changes in disease activity and are indicators of disease flare. Serum samples were obtained from Vardhman Mahavir Medical College and Safdarjung hospital. Serum samples were tested for the presence of ANA. The dsDNA and complement levels were done in the suspected SLE patients. The aim of this study was to find out the prevalence of ANA and anti dsDNA using Elisa in patient with suspected autoimmune disease along with the level of complement proteins by Nephleometry technique. Newer techniques like immune florescence can be used for better results.
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