Progress Towards a Higher Sensitivity <sup>13</sup>C-Optimized 1.5 mm HTS NMR Probe

Thomas, J. N.; Ramaswamy, V.; Litvak, Ilya M.; Johnston, T L; Edison, Arthur S.; Brey, Wallace S.

doi:10.1109/tasc.2021.3061042

Cited by 8 publications

(30 citation statements)

References 18 publications

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“…Even smaller, room temperature 1 mm solenoid coils can be used for metabolite structure validation using microgram sample quantities [ 106 , 107 ]. Finally, both cryogenic technology and optimized coil materials, such as high-temperature superconducting material, can be combined to enhance 13 C detection [ 39 , 108 , 109 ]. 13 C detection does not suffer from the same salt effects that limit 5 mm 1 H cryoprobes because 13 C frequencies are only a quarter of 1 H frequencies at the same field strength.…”

Section: Hardware Sensitivity Enhancementmentioning

confidence: 99%

NMR and Metabolomics—A Roadmap for the Future

et al. 2022

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Metabolomics investigates global metabolic alterations associated with chemical, biological, physiological, or pathological processes. These metabolic changes are measured with various analytical platforms including liquid chromatography-mass spectrometry (LC-MS), gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR). While LC-MS methods are becoming increasingly popular in the field of metabolomics (accounting for more than 70% of published metabolomics studies to date), there are considerable benefits and advantages to NMR-based methods for metabolomic studies. In fact, according to PubMed, more than 926 papers on NMR-based metabolomics were published in 2021—the most ever published in a given year. This suggests that NMR-based metabolomics continues to grow and has plenty to offer to the scientific community. This perspective outlines the growing applications of NMR in metabolomics, highlights several recent advances in NMR technologies for metabolomics, and provides a roadmap for future advancements.

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Section: Hardware Sensitivity Enhancementmentioning

confidence: 99%

NMR and Metabolomics—A Roadmap for the Future

et al. 2022

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“…High Q-factor high temperature superconducting (HTS) resonators have demonstrated remarkable detection sensitivity in comparison to normal-metal resonators [2][3][4][5][6]. We report here the in-field resonator performance and initial validation of a higher sensitivity 1.5 mm all-HTS NMR probe optimized for 13 C detection. A 1.5 mm 13 C-optimized all-HTS probe [2] of similar design (but also including a 15 N channel) previously developed in our lab has shown to be a powerful tool for metabolomics applications [7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…We report here the in-field resonator performance and initial validation of a higher sensitivity 1.5 mm all-HTS NMR probe optimized for 13 C detection. A 1.5 mm 13 C-optimized all-HTS probe [2] of similar design (but also including a 15 N channel) previously developed in our lab has shown to be a powerful tool for metabolomics applications [7][8][9][10][11]. In development of the new probe, advances in coil design and probe construction technique led to significantly higher resonator Qfactors [12,13].…”

Section: Introductionmentioning

confidence: 99%

“…A 1.5 mm 13 C-optimized all-HTS probe [2] of similar design (but also including a 15 N channel) previously developed in our lab has shown to be a powerful tool for metabolomics applications [7][8][9][10][11]. In development of the new probe, advances in coil design and probe construction technique led to significantly higher resonator Qfactors [12,13]. However, the leap in resonator Q-factor has led to the encounter of new obstacles to overcome in their implementation.…”

Section: Introductionmentioning

confidence: 99%

“…The purpose of this work is to first outline these obstacles and discuss techniques used to overcome them. The combined results of innovative HTS probe technology and the new implementation techniques discussed here have produced a 13 C NMR probe with sensitivity improvements over the previous probe while preserving signal bandwidth.…”

Section: Introductionmentioning

confidence: 99%

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Implementing High Q-Factor HTS Resonators to Enhance Probe Sensitivity in ¹³C NMR Spectroscopy

Thomas

Johnston

Litvak

et al. 2022

J. Phys.: Conf. Ser.

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Nuclear magnetic resonance spectroscopy (NMR) probes using thin-film high temperature superconducting (HTS) resonators provide exceptional mass sensitivity in small-sample NMR experiments for natural products chemistry and metabolomics. We report improvements in sensitivity to our 1.5 mm 13C-optimized NMR probe based on HTS resonators. The probe has a sample volume of 35 microliters and operates in a 14.1 T magnet. The probe also features HTS resonators for 1H transmission and detection and the 2H lock. The probe utilizes a 13C resonator design that provides greater efficiency than our previous design. The quality factor of the new resonator in the 14.1 T background field was measured to be 4,300, which is over 3x the value of the previous design. To effectively implement the improved quality factor, we demonstrate the effect of adding a shorted transmission line stub to increase the bandwidth and reduce the rise/fall time of 13C irradiation pulses. Initial NMR measurements verify 13C NMR sensitivity is significantly improved while preserving detection bandwidth. The probe will be used for applications in metabolomics.

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