Metabolomics in Cell Biology

Eraslan, Zuhal; Cascante, Marta; Günther, Ulrich L.

doi:10.1007/164_2022_619

Cited by 4 publications

(4 citation statements)

References 93 publications

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“…Metabolomics is a methodology focused on the analysis of all the metabolites (i.e., small molecules with a molecular weight <2 kDa, according to the definition of the Human Metabolome Database [5]) that are present in a biological sample. This technique has the ability to bring to light variations in the levels of intra-and extra-cellular metabolites induced by drug treatments and these changes can be directly related to the up-or down-regulation of specific pathways involved in the drug's modes of action [3,[6][7][8][9]. This methodological approach also offers the possibility to identify specific markers of tumor responsiveness or development of resistance to anticancer metal complexes.…”

Section: Nmr-based Cell Metabolomicsmentioning

confidence: 99%

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Cell Metabolomics to Guide the Design of Metal-Based Compounds

Ghini

2024

Inorganics

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Despite the increasing interest in the development of novel metal-based compounds for cancer treatment, these molecules are currently poorly characterized in mechanistic terms, due to their multiple macromolecular targets inside the cells. In this review, we show how 1H NMR metabolomics provides a powerful tool to investigate the metabolic perturbations induced by metal-compounds in cells. The chemical identity and concentration of metabolites detected in cell lysates and their respective growth media by NMR can be viewed as a global fingerprint that describes the response to drug treatment. In this framework, the applications of NMR-based metabolomics to study cellular effects induced by the treatment of cells with anticancer metal-based compounds are comprehensively reviewed.

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Section: Nmr-based Cell Metabolomicsmentioning

confidence: 99%

“…−40 to −50 • C). Subsequently, chloroform is added to separate the two phases, i.e., polar and non-polar extracts [6,7,14].…”

Section: Nmr-based Cell Metabolomicsmentioning

confidence: 99%

Cell Metabolomics to Guide the Design of Metal-Based Compounds

Ghini

2024

Inorganics

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“…4,10 NMR metabolomics is a very informative research tool capable of revealing the main metabolic alterations caused by a variety of drugs in cancer cells. 11,12 Recently, we have implemented this investigative approach to characterise the metabolic perturbations induced by a few metallodrugs in a reference cancer cell line. 13,14 We addressed the cellular alterations caused by AF in A2780 ovarian cancer cells revealing a large increase of intracellular glutathione concentration as the main effect of the treatment; 13 in line with these ndings, a subsequent proteomic analysis has shown that AF treatment in A2780 cells induces the overexpression of two enzymes involved in GSH biosynthesis, namely GCLC (glutamate-cysteine ligase catalytic subunit) and GCLM (glutamate-cysteine ligase regulatory subunit).…”

mentioning

confidence: 99%

“…NMR metabolomics is a very informative research tool capable of revealing the main metabolic alterations caused by a variety of drugs in cancer cells. 11,12 Recently, we have implemented this investigative approach to characterise the metabolic perturbations induced by a few metallodrugs in a reference cancer cell line. 13,14…”

mentioning

confidence: 99%