Progress in the study of virus detection methods: The possibility of alternative methods to validate virus inactivation

Qu, Shuxin; Xu, Liming

doi:10.1002/bit.27003

Cited by 29 publications

(35 citation statements)

References 58 publications

(146 reference statements)

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“…Compartmentalization of target DNA improves the signal to noise ratio by amplifying target gene signals relative to other genes. 16 In normal bulk PCR reactions, inhibitors or excess background DNA (noise) can affect amplification effectiveness. In ddPCR, as a binary system, the absolute quantification is acquired by counting the number of positive and negative fluorescence droplets, however in qPCR, as an analog system, fluorescence signal increases proportion to the amount of replicated DNA.…”

Section: Droplet Digital Pcr Versus Conventional Qpcr Methodsmentioning

confidence: 99%

“…15 Droplet-based digital PCR (ddPCR) is a type of digital PCR that employs one immiscible fluid (i.e., the so-called dispersed fluid) in oil (i.e., the so-called continuous fluid) to generate submicroliter droplets at kilohertz rates. 16,17 Nucleic acids, such as DNA, RNA, and complementary DNA (cDNA), may be encapsulated stochastically inside the droplets as reaction chambers. A small percentage of the droplets contain one or fewer copies of the DNA template (many of the droplets do not include any of the target DNA) and are then clonally amplified in each microdroplet.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Droplet digital PCR of viral ‎DNA/RNA, current progress, challenges, and future perspectives

Kojabad

Farzanehpour

Galeh

et al. 2021

Journal of Medical Virology

135

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High‐throughput droplet‐based digital PCR (ddPCR) is a refinement of the conventional polymerase chain reaction (PCR)‎ methods. In ddPCR, DNA/RNA is encapsulated stochastically inside the microdroplets as reaction chambers. A small percentage of the reaction chamber contains one or fewer copies of the DNA or RNA. After PCR amplification, concentrations are determined based on the proportion of nonfluorescent partitions through the Poisson distribution. Some of the main features of ddPCR include high sensitivity and specificity, absolute quantification without a standard curve, high reproducibility, good tolerance to PCR inhibitor, and high efficacy compared to conventional molecular methods. These advantages make ddPCR a valuable addition to the virologist's toolbox. The following review outlines the recent technological advances in ddPCR methods and their applications in viral identification.

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Section: Droplet Digital Pcr Versus Conventional Qpcr Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Droplet digital PCR of viral ‎DNA/RNA, current progress, challenges, and future perspectives

Kojabad

Farzanehpour

Galeh

et al. 2021

Journal of Medical Virology

135

View full text Add to dashboard Cite

show abstract

“… 23 Regarding the improvement of virus detection efficiency, due to their high surface area and ultrasmall size, NPs have been applied not only in RT-PCR methods but also other virus detection methods, such as an enzyme-linked immunosorbent assay (ELISA) and reverse transcription loop-mediated isothermal amplification (RT-LAMP). 24 , 25 Various kinds of NPs have been studied in the context of virus detection, including metal NPs, carbon nanotubes, silica NPs, quantum dots (QDs), and polymeric NPs. 20 , 26 Among them, metal NPs, metal nanoislands (NIs), magnetic NPs (MNPs), and QDs have been applied to coronavirus detection.…”

Section: Nanoparticles For Diagnosticsmentioning

confidence: 99%

Nanoparticle-Based Strategies to Combat COVID-19

Medhi

Srinoi

Ngo

et al. 2020

ACS Appl. Nano Mater.

172

161

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Coronavirus disease 2019 (COVID-19) is the worst pandemic disease of the current millennium. This disease is caused by the highly contagious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which first exhibited human-to-human transmission in December 2019 and has infected millions of people within months across 213 different countries. Its ability to be transmitted by asymptomatic carriers has put a massive strain on the currently available testing resources. Currently, there are no clinically proven therapeutic methods that clearly inhibit the effects of this virus, and COVID-19 vaccines are still in the development phase. Strategies need to be explored to expand testing capacities, to develop effective therapeutics, and to develop safe vaccines that provide lasting immunity. Nanoparticles (NPs) have been widely used in many medical applications, such as biosensing, drug delivery, imaging, and antimicrobial treatment. SARS-CoV-2 is an enveloped virus with particle-like characteristics and a diameter of 60–140 nm. Synthetic NPs can closely mimic the virus and interact strongly with its proteins due to their morphological similarities. Hence, NP-based strategies for tackling this virus have immense potential. NPs have been previously found to be effective tools against many viruses, especially against those from the Coronaviridae family. This Review outlines the role of NPs in diagnostics, therapeutics, and vaccination for the other two epidemic coronaviruses, the 2003 severe acute respiratory syndrome (SARS) virus and the 2012 Middle East respiratory syndrome (MERS) virus. We also highlight nanomaterial-based approaches to address other coronaviruses, such as human coronaviruses (HCoVs); feline coronavirus (FCoV); avian coronavirus infectious bronchitis virus (IBV); coronavirus models, such as porcine epidemic diarrhea virus (PEDV), porcine reproductive and respiratory syndrome virus (PRRSV), and transmissible gastroenteritis virus (TGEV); and other viruses that share similarities with SARS-CoV-2. This Review combines the salient principles from previous antiviral studies with recent research conducted on SARS-CoV-2 to outline NP-based strategies that can be used to combat COVID-19 and similar pandemics in the future.

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“…Nanomedicine is used not only in PCR setting, but also in enzyme-linked immunosorbent assay (ELISA) and reverse transcription loop-mediated isothermal amplification (RT-LAMP) [185]:…”

Section: Nano For Diagnosis Of Covid-19mentioning

confidence: 99%

Nanomedicine for COVID-19: the role of nanotechnology in the treatment and diagnosis of COVID-19

Vahedifard

Chakravarthy

2021

emergent mater.

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused the recent outbreak of coronavirus 2019 (COVID-19). Although nearly two decades have passed since the emergence of pandemics such as SARS-CoV and Middle East respiratory syndrome coronavirus (MERS-CoV), no effective drug against the CoV family has yet been approved, so there is a need to find newer therapeutic targets. Currently, simultaneous research across the globe is being performed to discover efficient vaccines or drugs, including both conventional therapies used to treat previous similar diseases and emerging therapies like nanomedicine. Nanomedicine has already proven its value through its application drug delivery and nanosensors in other diseases. Nanomedicine and its components can play an important role in various stages of prevention, diagnosis, treatment, vaccination, and research related to COVID-19. Nano-based antimicrobial technology can be integrated into personal equipment for the greater safety of healthcare workers and people. Various nanomaterials such as quantum dots can be used as biosensors to diagnose COVID-19. Nanotechnology offers benefits from the use of nanosystems, such as liposomes, polymeric and lipid nanoparticles, metallic nanoparticles, and micelles, for drug encapsulation, and facilitates the improvement of pharmacological drug properties. Antiviral functions for nanoparticles can target the binding, entry, replication, and budding of COVID-19. The toxicity-related inorganic nanoparticles are one of the limiting factors of its use that should be further investigated and modified. In this review, we are going to discuss nanomedicine options for COVID-19 management, similar applications for related viral diseases, and their gap of knowledge.

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Progress in the study of virus detection methods: The possibility of alternative methods to validate virus inactivation

Cited by 29 publications

References 58 publications

Droplet digital PCR of viral ‎DNA/RNA, current progress, challenges, and future perspectives

Droplet digital PCR of viral ‎DNA/RNA, current progress, challenges, and future perspectives

Nanoparticle-Based Strategies to Combat COVID-19

Nanomedicine for COVID-19: the role of nanotechnology in the treatment and diagnosis of COVID-19

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