Progress in the genetic engineering of cereals to produce essential polyunsaturated fatty acids

Kraic, Ján; Mihálik, Daniel; Klčová, Lenka; Gubišová, Marcela; Klempová, Tatiana; Hudcovicová, Martina; Ondreičková, Katarína; Mrkvová, Michaela; Havrlentová, Michaela; Gubiš, Jozef; Čertı́k, Milan

doi:10.1016/j.jbiotec.2018.08.009

Cited by 23 publications

(12 citation statements)

References 116 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even though the niger sample presented the lowest amount of fatty acids found, it can be considered the most promising due to its high content in PUFA-specifically, linoleic acid. According to some studies, an insufficient intake of polyunsaturated fatty acids is directly related to the increased possibility of the onset of diseases such as cardiovascular, brain, inflammatory and cancer [45]. The results obtained were confirmed by other studies, which carried out the quantification of fatty acids in niger seeds from other regions of the world.…”

Section: Nutritional Profile and Chemical Composition Of The Three Flour Seeds Samplessupporting

confidence: 59%

Characterization of Nonconventional Food Plants Seeds Guizotia abyssinica (L.f.) Cass., Panicum miliaceum L., and Phalaris canariensis L. for Application in the Bakery Industry

et al. 2021

View full text Add to dashboard Cite

Consumers have given preferences to food products that, in addition to the nutritional properties, also present bioactive characteristics with beneficial health effects. The use of Nonconventional Food Plants (NCFP) has been an asset for the food industry, not only due to its abundance but, also, because it does not compete with other vegetable matrices used for human consumption for its nutritional properties, chemical and bioactive potentiality. The present work aimed to study the granulometry and water absorption index of three seeds of unconventional food plants: niger, millet and birdseed, followed by their nutritional value, content in free sugars, fatty acids, organic acids, tocopherols and phenolic compounds by chromatographic methods and, also, the evaluation of the antioxidant, hepatotoxic and antimicrobial potential in their hydroethanolic extracts. Bakery products were developed with a partial replacement of wheat flour, using the centroid simplex method to understand the effect of applying NCFP flours in the final physical–chemical characteristics. The high granulometry associated with the high water absorption index indicates that the use of NCFP flours would have to be complemented with other flours for baking. The chemical composition of niger seed stood out the most; however, all seeds presented relatively low IC50 and MIC values for the inhibition of lipid peroxidation and antimicrobial activity, respectively. Finally, breads made with a mixture of millet and birdseed flour showed the greatest similarity to the control bread. Considering their composition in bioactive compounds, the use of these seeds is highly advisable in the context of a fortified diet, being sources of compounds of high nutritional value and with beneficial health effects for the final consumer.

show abstract

Section: Nutritional Profile and Chemical Composition Of The Three Flour Seeds Samplessupporting

confidence: 59%

Characterization of Nonconventional Food Plants Seeds Guizotia abyssinica (L.f.) Cass., Panicum miliaceum L., and Phalaris canariensis L. for Application in the Bakery Industry

et al. 2021

View full text Add to dashboard Cite

show abstract

“…36 Studies have reported that insufficient consumption of PUFA increases the probability of age-related diseases, such as cardiovascular, cancer, brain, and inflammatory diseases, deficiency symptoms, and cognitive, psychiatric, antisocial, and other disorders. 37 To the authors' best knowledge there are no previous reports regarding the fatty acid composition in the red rubin basil variety.…”

Section: Chemical Composition Of Leavesmentioning

confidence: 99%

Ocimum basilicum var. purpurascens leaves (red rubin basil): a source of bioactive compounds and natural pigments for the food industry

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Plant oils are important renewable resources and have played a central role in human existence for many thousands of years (Dyer et al ., 2008; Napier and Graham, 2010). With increasing demand for food, industrial feedstock and fuel, biotechnology strategies have been applied to improve unusual fatty acid (UFA) content in oilseed plants (Napier and Graham, 2010; Iskandarov et al ., 2017; Reynolds et al ., 2017; Kraic et al ., 2018; Reynolds et al ., 2019). Current modifications include those of medium chain FAs (Kim et al ., 2015; Iskandarov et al ., 2017; Reynolds et al ., 2017 and 2019), very long chain FAs (Wu et al ., 2005; Xue et al ., 2013; Ruiz‐Lopez et al ., 2015; Walsh et al ., 2016), conjugated FAs (van Erp et al ., 2015; Yurchenko et al ., 2017), hydroxy FAs (Lu et al ., 2006; van Erp et al ., 2011; Yu et al ., 2014; Aryal and Lu, 2018; Li et al ., 2018; Lunn et al ., 2018 and 2019) and cyclopropane FAs (Yu et al ., 2014 and 2018).…”

Section: Introductionmentioning

confidence: 99%

Ultra‐high α‐linolenic acid accumulating developmental defective embryo was rescued by lysophosphatidic acid acyltransferase 2

et al. 2020

View full text Add to dashboard Cite

Summary For decades, genetic engineering approaches to produce unusual fatty acids (UFAs) in crops has reached a bottleneck, including reduced seed oil production and seed vigor. Currently, plant models in the field of research are primarily used to investigate defects in oil production and seedling development, while the role of UFAs in embryonic developmental defects remains unknown. In this study, we developed a transgenic Arabidopsis plant model, in which the embryo exhibits severely wrinkled appearance owing to α‐linolenic acid (ALA) accumulation. RNA‐sequencing analysis in the defective embryo suggested that brassinosteroid synthesis, FA synthesis and photosynthesis were inhibited, while FA degradation, endoplasmic reticulum stress and oxidative stress were activated. Lipidomics analysis showed that ultra‐accumulated ALA is released from phosphatidylcholine as a free FA in cells, inducing severe endoplasmic reticulum and oxidative stress. Furthermore, we identified that overexpression of lysophosphatidic acid acyltransferase 2 rescued the defective phenotype. In the rescue line, the pool capacity of the Kennedy pathway was increased, and the esterification of ALA indirectly to triacylglycerol was enhanced to avoid stress. This study provides a plant model that aids in understanding the molecular mechanism of embryonic developmental defects and generates strategies to produce higher levels of UFAs.

show abstract

Progress in the genetic engineering of cereals to produce essential polyunsaturated fatty acids

Cited by 23 publications

References 116 publications

Characterization of Nonconventional Food Plants Seeds Guizotia abyssinica (L.f.) Cass., Panicum miliaceum L., and Phalaris canariensis L. for Application in the Bakery Industry

Characterization of Nonconventional Food Plants Seeds Guizotia abyssinica (L.f.) Cass., Panicum miliaceum L., and Phalaris canariensis L. for Application in the Bakery Industry

Ocimum basilicum var. purpurascens leaves (red rubin basil): a source of bioactive compounds and natural pigments for the food industry

Ultra‐high α‐linolenic acid accumulating developmental defective embryo was rescued by lysophosphatidic acid acyltransferase 2

Contact Info

Product

Resources

About