Progress in the accuracy of mycotoxin analysis in the last quarter century

Dou

et al. 2018

Toxins

165

The presence of mycotoxins in herbal medicines is an established problem throughout the entire world. The sensitive and accurate analysis of mycotoxin in complicated matrices (e.g., herbs) typically involves challenging sample pretreatment procedures and an efficient detection instrument. However, although numerous reviews have been published regarding the occurrence of mycotoxins in herbal medicines, few of them provided a detailed summary of related analytical methods for mycotoxin determination. This review focuses on analytical techniques including sampling, extraction, cleanup, and detection for mycotoxin determination in herbal medicines established within the past ten years. Dedicated sections of this article address the significant developments in sample preparation, and highlight the importance of this procedure in the analytical technology. This review also summarizes conventional chromatographic techniques for mycotoxin qualification or quantitation, as well as recent studies regarding the development and application of screening assays such as enzyme-linked immunosorbent assays, lateral flow immunoassays, aptamer-based lateral flow assays, and cytometric bead arrays. The present work provides a good insight regarding the advanced research that has been done and closes with an indication of future demand for the emerging technologies.

Section: Analytical Techniques Of Mycotoxinsmentioning

confidence: 99%

A Review of Current Methods for Analysis of Mycotoxins in Herbal Medicines

Dou

et al. 2018

Toxins

165

“…The rise in demand for data accuracy, separation and quantitation of aflatoxin analysis made people to convert to high performance liquid chromatographic compared to thin layer chromatographic ( 24 ). Honma et al ( 29 ) conducted a review that showed that aflatoxin B 1 in maize samples were analyzed using thin layer chromatographic technique in 1978 ( 24 ). The study also showed that the usage of thin layer chromatographic technique has decreased to 48% in 1989 and to 7% in 2002.…”

Section: Introductionmentioning

confidence: 99%

Aflatoxin tests in herbal products and its quantification: Latest updates

Jeyaraj

Loy²,

Goh³

et al. 2022

Front. Nutr.

Aflatoxin is naturally occurring mycotoxins produced by fungi. The existence of aflatoxin in herbal medicines is a well-known issue. The detection of aflatoxin with good sensitivity and also that is reliable in complex matrices like herbs usually necessitates difficult processes and powerful detection instrument in preparation of sample. This study investigated the global occurrence of aflatoxin contamination herbal products. This article pivots on key breakthroughs in preparation of sample and its importance in analytical technology. Studies from published studies were screened to determine the general level of aflatoxin contamination. The countries involved were Malaysia, Indonesia, Kenya, Brazil, Nigeria, Thailand, South Africa, and Morocco. This review also includes recent studies on the development and application of screening assays such as lateral flow immunoassays, enzyme-linked immunosorbent assays, aptamer-based lateral flow assays, and cytometric bead arrays, as well as traditional chromatographic techniques for aflatoxin qualification or quantitation. The current study looks at aflatoxin contamination of key herbal drug raw material, which are frequently used in the production of numerous herbal pharmaceuticals. Contamination of aflatoxin might occur in herbal products if the ingredients such as medicinal herbs and plants that are used in manufacturing of herbal products are not dried thoroughly or stored inappropriately after preparation.

Food Additives & Contaminants: Part A

Aflatoxin measurements: how HPLC methods have evolved over the last 20 years?

Buttinger

2010

The Institute for Reference Materials and Measurements (IRMM) has provided high quality certified reference materials for decades. Certified reference materials for mycotoxin analysis have been available from the IRMM since 1985. Since then, IRMM has generally characterised reference materials by interlaboratory studies with expert laboratories. Such measurement exercises are based on routinely used analytical procedures in the laboratory, but are driving their performance to the best achievable quality under the given circumstances. Reference materials for aflatoxin measurements have been certified by such studies in 1991, 1993, 2008 and 2009. After two decades, it is time to look back, reflect and ask some questions. How have HPLC methods for the analysis of aflatoxins evolved over the last 20 years? Is there a trend towards a preferred methodology? Does this evolution reflect improvements due to the introduction of standardised methods? Is there an improvement in performance parameters such as repeatability, reproducibility and measurement uncertainty? To answer these questions, data from six interlaboratory studies, performed in the last 20 years for the certification of aflatoxins in peanut meal, peanut butter and animal feed, have been re-evaluated. It is concluded that there is a trend towards a preferred methodology, which reflects developments in standardised methods. No significant improvement in repeatability, reproducibility or measurement uncertainty could, however, be detected in expert laboratories for mycotoxin analysis.