Progress in Plant Polyploidization Based on Antimicrotubular Drugs

Yemets, А. І.; Blume, Ya. B.

doi:10.2174/1874840600801010015

Cited by 30 publications

(19 citation statements)

References 67 publications

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“…Colchicine was first identified in 1937 as having the ability to induce chromosome doubling (Blakeslee and Avery 1937). Since then, treatment of plant tissues with colchicine and a variety of other antimitotic drugs has been the most widely used method of inducing polyploidy (Hancock 1997;Wu and Mooney 2002;Yemets and Blume 2008;Dhooghe et al 2010). Chromosome doubling has been used after successful hybridization to restore the fertility of plants with unbalanced numbers of genomes (Ortiz 1998;Rose et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Manipulation of ploidy for kiwifruit breeding: in vitro chromosome doubling in diploid Actinidia chinensis Planch.

Ferguson

Murray

2011

Plant Cell Tiss Organ Cult

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Tetraploid plants were induced by colchicine treatment of in vitro leaf petiole segment cultures of five diploid Actinidia chinensis Planch. genotypes, including the commercially important, yellow-fleshed cultivar 'Hort16A', three female selections with red-fleshed fruit and one male pollinizer. Petiole segments were incubated on a shoot regeneration medium for a period of 4 weeks, and subsequently microshoots were treated with 0.05 or 0.1% colchicine. About one-third of the regenerated shoots were tetraploid following 0.05% colchicine treatment, more than with 0.1% colchicine treatment. Similar rates of tetraploid induction were achieved with all the genotypes tested. The efficiency of induction of polyploidy depended on the interaction between the types of in vitro culture chosen and the concentration of colchicine used. There are no previous reports of colchicine being used so successfully to induce polyploidy in Actinidia.

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Section: Introductionmentioning

confidence: 99%

Manipulation of ploidy for kiwifruit breeding: in vitro chromosome doubling in diploid Actinidia chinensis Planch.

Ferguson

Murray

2011

Plant Cell Tiss Organ Cult

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“…Many researchers have induced polyploidy in several plant species through the antimitotic chemical, colchicine (C 22 H 25 NO 6 ) [8][9][10] which is obtained from the seeds and bulbs of the plant Colchicum autumnale L. More importantly without damaging any other life processes of the chromosomes, colchicine effects a temporary inactivation of the spindle mechanism, both the exterior part working from the centrosomes and the interior centromeric part [11]. Among various antimicrotubular drugs colchicine has proven to be the most useful to double the chromosome numbers of numerous crop species, including decorative flowers, medical and agricultural plants [12].…”

mentioning

confidence: 99%

Induced polyploidization in Brassica campestris L. (Brassicaceae)

Kumar

Dwivedi

2014

Cytol. Genet.

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“…research evidence now exists, which details polyploidy production using alternative mitosis inhibitors (Yemets, Blume, 2008). The various biotechnological techniques perfected over the last century have been also used for the polyploidization of different plant species in the in vitro systems.…”

Section: Production Of Trifolium Pratense L and T Hybridum L Tetramentioning

confidence: 99%

“…Yemets and Blume (2008) have reported that already in 1979 tetraploid populations of as many as 150 plant species were produced by colchicine treatment. However, it was found that colchicine not only doubles the number of chromosomes but also causes undesirable mutations and is poisonous to humans.…”

Section: Introductionmentioning

confidence: 99%

Production of Trifolium pratense L. and T. hybridum L. tetraploid populations and assessment of their agrobiological characteristics

Dabkevičienė

Statkevičiūtė

Mikaliūnienė

et al. 2016

Zemdirbyste-Agriculture

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The current study was set out to establish the efficacy of different mitosis inhibitors (colchicine, amiprophos-methyl, trifluralin and oryzalin) used in the production of red clover (Trifolium pratense L.) and alsike clover (Trifolium hybridum L.) polyploids and to assess the agrobiological traits of tetraploid populations. Four cultivars and five wild populations of T. pratense, one cultivar and three wild populations of T. hybridum were used for polyploidization. The effect of conventional soaking of germinating seeds in a colchicine solution was compared with that of colchicine treatment of embryos in an in vitro culture. Colchicine treatment of T. pratense embryos resulted in 3.3 times higher production of tetraploids (teraploid yield 55.0%) compared with colchicine treatment of seedlings, and the production of chimeric plants was 1.9 times lower. The different mitosis inhibitors were characterised by a diverse polyploidization effect. Tetraploid yield depended not only on the mitosis inhibitor used but also on the plant species. Treatment of T. pratense embryos with colchicine, amiprophosmethyl and oryzalin yielded similar results in terms of tetraploid production (31.3-40.7%) and chimeras (14.3-22.4%). The concentration of trifluralin used for the production of T. pratense tetraploids was ineffective. Colchicine solution was more efficient for the development of T. hybridum tetraploids whose yield was 2.5 times as high as that obtained having treated the embryos with amiprophos-methyl. Induced tetraploid populations were compared to reference cultivars in field trials. The two T. pratense populations 'Radviliai 4n' and 'Arimaičiai 4n' stood out in this respect -their plants accumulated higher dry matter contents, produced more inflorescences than those of the reference cultivar and exhibited prolific seed yield capacity. In relation to the agrobiological traits, the tetraploid populations of T. hybridum did not surpass the reference cultivar.

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Progress in Plant Polyploidization Based on Antimicrotubular Drugs

Cited by 30 publications

References 67 publications

Manipulation of ploidy for kiwifruit breeding: in vitro chromosome doubling in diploid Actinidia chinensis Planch.

Manipulation of ploidy for kiwifruit breeding: in vitro chromosome doubling in diploid Actinidia chinensis Planch.

Induced polyploidization in Brassica campestris L. (Brassicaceae)

Production of Trifolium pratense L. and T. hybridum L. tetraploid populations and assessment of their agrobiological characteristics

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