Progress in optical sensors-based uric acid detection

A facile, low-cost bimetallic iron–nickel MOF nanozyme-propelled ratiometric fluorescent sensor for highly sensitive and selective uric acid detection and its smartphone application

“…4–6 Moreover, there are also reports that low levels of UA could also cause multiple sclerosis, diabetes mellitus and even genetic disorders. 7 Therefore, it is highly significant to establish effective methods for UA detection. Currently, tremendous efforts are being made to monitor the level of UA using methods such as high-performance liquid chromatography (HPLC), 8 liquid chromatography-tandem mass spectrometry (LC-MS/MS), 9 colorimetry, 10,11 electrochemistry, 12,13 fluorometry, 14 electrochemical-surface enhanced Raman spectroscopy (EC-SERS), 15,16 long range surface plasmon resonance (LRSPR) method 17 and so on.…”

Development of a facile electrochemical sensor based on GCE modified with one-step prepared PNMA-CeO2-fMWCNTs composite for simultaneous detection of UA and 5-FU

“…Various analytical methods have been developed in the literature to detect UA and 5-FU analytes in biological samples. 9,14–20 Compared to other analytical approaches, the electrochemical methods can allow the detection of the analytes in biological samples without a complex pretreatment method. 12,21 Electrochemical techniques have remarkable features such as high sensitivity, fast response time, low cost, instrumental simplicity, and possible miniaturization and integration into portable devices.…”

Simple enzyme-free detection of uric acid by an in situ fluorescence and colorimetric method based on Co-PBA with high oxidase activity

“…They usually need the auxiliary effect of uricase to produce unstable H 2 O 2 as an intermediate for the amplification of signals, and the oxidation of 3,3′,5,5′-tetramethylbenzidine (ox-TMB) by the catalytic action of peroxidase mimics to trigger the signal change further. 14 Liang et al 15 proposed a method for colorimetric quantification of UA based on the urease-immobilized peroxidase-like mimic PCN-222(Fe) and enzyme cascade strategy, but this method requires expensive and unstable natural enzymes to participate in the reaction, which is even more demanding in terms of reaction conditions. In contrast, Wang et al 10 used Ag 5 PMo 12 @PPy to simulate the properties of peroxidase and established a UA colorimetric detection platform, which avoids the use of natural enzymes, simplifies the operational steps, and achieves a more rapid UA assay.…”