Progress in characterization, preparation and clinical applications of non-hematopoietic stem cells, 29–30 September 2006, Tübingen, Germany

Schäfer, Richard; Dominici, Massimo; Müller, Ingo; Dazzi, Francesco; Godthardt, K.; Blanc, Katarina Le; Meisel, Roland; Pochampally, Radhika; Richter, Rudolf; Skutella, Thomas; Steinhoff, Gustav; Mitterberger, Michael; Wendel, Hans-Peter; Wiskirchen, Jakub; Handgretinger, Rupert; Northoff, Hinnak

doi:10.1080/14653240701392949

Cited by 22 publications

(15 citation statements)

References 60 publications

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“…Mesenchymal stem cells are functionally characterized by in vitro differentiation assays. 43 We evaluated the basic differentiation potential into three mesenchymal lineages: adipogenic, osteogenic and chondrogenic differentiation. The different hMSC populations were each treated either with adipogenic medium, osteogenic medium or normal medium (control) for 21 days, as previously described 44 (modification: no addition of amphotericin B).…”

Section: Discussionmentioning

confidence: 99%

Survival, neuron-like differentiation and functionality of mesenchymal stem cells in neurotoxic environment: the critical role of erythropoietin

et al. 2009

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Mesenchymal stem cells (MSCs) can ameliorate symptoms in several neurodegenerative diseases. However, the toxic environment of a degenerating central nervous system (CNS) characterized by hypoxia, glutamate (Glu) excess and amyloid beta (Abeta) pathology may hamper the survival and regenerative/replacing capacities of engrafted stem cells. Indeed, human MSC (hMSC) exposed to hypoxia were disabled in (i) the capacity of their muscarinic receptors (mAChRs) to respond to acetylcholine (ACh) with a transient increase in intracellular [Ca 2 þ ], (ii) their capacity to metabolize Glu, reflected by a strong decrease in glutamine synthetase activity, and (iii) their survival on exposure to Glu. Cocultivation of MSC with PC12 cells expressing the amyloid precursor protein gene (APPsw-PC12) increased the release of IL-6 from MSC. HMSC exposed to erythropoietin (EPO) showed a cholinergic neuron-like phenotype reflected by increased cellular levels of choline acetyltransferase, ACh and mAChR. All their functional deficits observed under hypoxia, Glu exposure and APPsw-PC12 cocultivation were reversed by the application of EPO, which increased the expression of Wnt3a. EPO also enhanced the metabolism of Abeta in MSC by increasing their neprilysin content. Our data show that cholinergic neuron-like differentiation of MSC, their functionality and resistance to a neurotoxic environment is regulated and can be improved by EPO, highlighting its potential for optimizing cellular therapies of the CNS.

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Section: Discussionmentioning

confidence: 99%

Survival, neuron-like differentiation and functionality of mesenchymal stem cells in neurotoxic environment: the critical role of erythropoietin

et al. 2009

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“…Alpha actinin: green; PKH26-labeled mMSCs: red; DAPI: blue; shaped arrowheads: mMSCs expressing alpha actinin; scale bars: 20 mm. that isolation of MSC by plastic adherence leads to a contamination of the MSC preparation with undesired cell types [56]. The hMSCs and mMSCs used in our study were extensively characterized by flow cytometry and tri-lineage differentiation; however, a clonal expansion of subpopulation(s) under different culture conditions with varying susceptibility to the in vitro differentiation stimuli cannot be excluded.…”

mentioning

confidence: 99%

Bone Marrow-Derived Human Mesenchymal Stem Cells Express Cardiomyogenic Proteins But Do Not Exhibit Functional Cardiomyogenic Differentiation Potential

Siegel¹,

Krause²,

Wöhrle³

et al. 2012

Stem Cells and Development

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Despite their paracrine activites, cardiomyogenic differentiation of bone marrow (BM)-derived mesenchymal stem cells (MSCs) is thought to contribute to cardiac regeneration. To systematically evaluate the role of differentiation in MSC-mediated cardiac regeneration, the cardiomyogenic differentiation potential of human MSCs (hMSCs) and murine MSCs (mMSCs) was investigated in vitro and in vivo by inducing cardiomyogenic and noncardiomyogenic differentiation. Untreated hMSCs showed upregulation of cardiac tropopin I, cardiac actin, and myosin light chain mRNA and protein, and treatment of hMSCs with various cardiomyogenic differentiation media led to an enhanced expression of cardiomyogenic genes and proteins; however, no functional cardiomyogenic differentiation of hMSCs was observed. Moreover, co-culturing of hMSCs with cardiomyocytes derived from murine pluripotent cells (mcP19) or with murine fetal cardiomyocytes (mfCMCs) did not result in functional cardiomyogenic differentiation of hMSCs. Despite direct contact to beating mfCMCs, hMSCs could be effectively differentiated into cells of only the adipogenic and osteogenic lineage. After intramyocardial transplantation into a mouse model of myocardial infarction, Sca-1 + mMSCs migrated to the infarcted area and survived at least 14 days but showed inconsistent evidence of functional cardiomyogenic differentiation. Neither in vitro treatment nor intramyocardial transplantation of MSCs reliably generated MSC-derived cardiomyocytes, indicating that functional cardiomyogenic differentiation of BM-derived MSCs is a rare event and, therefore, may not be the main contributor to cardiac regeneration.

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“…1). Due to the lack of a unique MSC function, these populations were termed 'mesenchymal stem cells' or synonymously 'marrow stromal cells', 'BM stromal cells' and 'mesenchymal stromal cells' [16][17][18]. Due to specific features which indicate more primitive subsets of MSCs with a higher differentiation capacity [reviewed in 19], some authors described the cells as 'multipotent adult progenitor cells' (MAPC) [20], 'marrowisolated adult multilineage inducible cells' (MIAMI) [21] or 'multipotent adult stem cells' (MASC) [22].…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal Stem or Stromal Cells: Toward a Better Understanding of Their Biology?

Lindner¹,

Kramer

Rohwedel

et al. 2010

Transfus Med Hemother

140

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Progress in characterization, preparation and clinical applications of non-hematopoietic stem cells, 29–30 September 2006, Tübingen, Germany

Cited by 22 publications

References 60 publications

Survival, neuron-like differentiation and functionality of mesenchymal stem cells in neurotoxic environment: the critical role of erythropoietin

Survival, neuron-like differentiation and functionality of mesenchymal stem cells in neurotoxic environment: the critical role of erythropoietin

Bone Marrow-Derived Human Mesenchymal Stem Cells Express Cardiomyogenic Proteins But Do Not Exhibit Functional Cardiomyogenic Differentiation Potential

Mesenchymal Stem or Stromal Cells: Toward a Better Understanding of Their Biology?

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