Programmable Nanostructures Based on Framework-DNA for Applications in Biosensing

Abstract

DNA has been actively utilized as bricks to construct exquisite nanostructures due to their unparalleled programmability. Particularly, nanostructures based on framework DNA (F-DNA) with controllable size, tailorable functionality, and precise addressability hold excellent promise for molecular biology studies and versatile tools for biosensor applications. In this review, we provide an overview of the current development of F-DNA-enabled biosensors. Firstly, we summarize the design and working principle of F-DNA-based nanodevices. Then, recent advances in their use in different kinds of target sensing with effectiveness have been exhibited. Finally, we envision potential perspectives on the future opportunities and challenges of biosensing platforms.

DNA and Nanomaterials: A Functional Combination for DNA Sensing

“…As aforementioned, the unique properties of DNA/nucleic acids and the nanomaterials’ intrinsic features have led to a keen interest to employ nucleic acids in the manufacture of reliable biosensors. DNA has played a key role in organisms and emerged in time with superior properties . DNA has emerged as a durable sensor component that led to the design of robust DNA sensors; similarly, DNA has been studied for its multiple advantageous roles such as mechanical stability, programmable stability, well-known thermal stability, and predictable reactions.…”

“…17 emerged in time with superior properties. 18 DNA has emerged as a durable sensor component that led to the design of robust DNA sensors; similarly, DNA has been studied for its multiple advantageous roles such as mechanical stability, programmable stability, well-known thermal stability, and predictable reactions. In earlier decades, DNA has been used to engineer scaffolds with mechanical stabilities in extreme conditions.…”

Special Issue “Feature Papers in Biosensors Section 2022”

“…Biosensors are devices composed of a biorecognition part and of a transduction part. Natural biomolecules such as enzymes [ 1 ], antibodies [ 2 ], DNA strains [ 3 ], bacteria and yeast [ 4 , 5 ], cells and even plant/animal tissues can constitute the recognition part. Synthesized polymers such as MIP (molecularly imprinted polymers) [ 1 ], aptamers and peptides can also be used as recognition part.…”

“…It was demonstrated that streptavidin is preferable to neutravidin for constructing lipid bilayers based sensing platforms [ 7 ]. Nanostructures based on framework DNA hold excellent promise for molecular biology studies and versatile tools for biosensor applications as reviewed in [ 3 ]. Dynamic mode decomposition of fluorescence loss was also used for monitoring protein diffusion, protein assemblies and protein aggregates in living cells [ 8 ].…”