Programmable DNA-Fueled Electrochemical Analysis of Lung Cancer Exosomes

Sha, Lingjun; Bo, Bing; Yang, Fan; Li, Jiayu; Cao, Ya; Zhao, Jing

doi:10.1021/acs.analchem.2c01318

Cited by 32 publications

(22 citation statements)

References 36 publications

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“…The method achieved cascade amplification of the signal through the trans-cleavage reaction promoted by PER and Cas12a, which improved the detection sensitivity. The detection range of exosomes was from 10 3 to 10 9 particles.mL -1 , with a low detection limit of 708 particles.mL -1 103 (Fig. 5 D) .…”

Section: Detection Methodsmentioning

confidence: 96%

See 1 more Smart Citation

Recent Advancement of PD-L1 Detection Technologies and Clinical Applications in the Era of Precision Cancer Therapy

Zhang¹,

Wu²,

Zhao³

et al. 2023

J. Cancer

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Programmed death-1 is a protein found on the surface of immune cells that can interact with its ligand, programmed death-ligand 1 (PD-L1), which is expressed on the plasma membrane, the surface of secreted cellular exosomes, in cell nuclei, or as a circulating soluble protein. This interaction can lead to immune escape in cancer patients. In clinical settings, PD-L1 plays an important role in tumor disease diagnosis, determining therapeutic effectiveness, and predicting patient prognosis. PD-L1 inhibitors are also essential components of tumor immunotherapy. Thus, the detection of PD-L1 levels is crucial, especially in the era of precision cancer therapy. In recent years, innovations have been made in traditional immunoassay methods and the development of new immunoassays for PD-L1 detection. This review aims to summarize recent research progress in tumor PD-L1 detection technology and highlight the clinical applications of PD-L1.

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Section: Detection Methodsmentioning

confidence: 96%

“…(D) Linear relationship between the peak currents and the logarithmic values of exosome concentrations. (A and B cited from reference 99 , with permission by Elsevier; C and D cited from reference 103 , with permission by ACS.)…”

Section: Figurementioning

confidence: 99%

Recent Advancement of PD-L1 Detection Technologies and Clinical Applications in the Era of Precision Cancer Therapy

Zhang¹,

Wu²,

Zhao³

et al. 2023

J. Cancer

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“…Finally, abundant Fc-labeled DNA fragments were released and adsorbed onto the MOF-modified electrode, producing a strong electrochemical signal. Meanwhile, Sha et al used a cucurbit [ 7 ] uril (a macrocyclic molecule)-modified electrode to capture the released methylene blue tags through the host–guest interactions [ 182 ]. In this work, programmed-death-1-expressing exosomes were first captured by anti-programmed death-ligand 1 antibody-modified MBs and then labeled with cholesterol-modified hairpin templates.…”

Section: Mb/mnp-based Target Conversionmentioning

confidence: 99%

Overview on the Design of Magnetically Assisted Electrochemical Biosensors

et al. 2022

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Electrochemical biosensors generally require the immobilization of recognition elements or capture probes on the electrode surface. This may limit their practical applications due to the complex operation procedure and low repeatability and stability. Magnetically assisted biosensors show remarkable advantages in separation and pre-concentration of targets from complex biological samples. More importantly, magnetically assisted sensing systems show high throughput since the magnetic materials can be produced and preserved on a large scale. In this work, we summarized the design of electrochemical biosensors involving magnetic materials as the platforms for recognition reaction and target conversion. The recognition reactions usually include antigen–antibody, DNA hybridization, and aptamer–target interactions. By conjugating an electroactive probe to biomolecules attached to magnetic materials, the complexes can be accumulated near to an electrode surface with the aid of external magnet field, producing an easily measurable redox current. The redox current can be further enhanced by enzymes, nanomaterials, DNA assemblies, and thermal-cycle or isothermal amplification. In magnetically assisted assays, the magnetic substrates are removed by a magnet after the target conversion, and the signal can be monitored through stimuli–response release of signal reporters, enzymatic production of electroactive species, or target-induced generation of messenger DNA.

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“…A myriad of methods have been developed for EV PD-L1 detection. − High-speed centrifugation can isolate EV PD-L1 from soluble PD-L1 protein by precipitation. , However, this is too time-consuming and cumbersome for clinical applications. Hence, a more convenient microfluidic affinity sorting method has been developed.…”

Section: Introductionmentioning

confidence: 99%

Reliable Detection of Extracellular PD-L1 by DNA Computation-Mediated Microfluidics

Zhang

Huang

et al. 2023

Anal. Chem.

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Extracellular vesicle PD-L1 (programmed death-1 ligand 1) is of greater value in tumor diagnosis, prognosis, and efficacy monitoring of anti-PD-1/PD-L1 immunotherapy. However, soluble PD-L1 interferes with the accurate detection of extracellular vesicle (EV) PD-L1. Here, we developed a microfluidic differentiation method for the detection of extracellular PD-L1, without the interference of soluble, by DNA computation with lipid probes and PD-L1 aptamer as inputs (DECLA). For the developed DECLA method, a cholesterol-DNA probe was designed that efficiently embeds into the EV membrane, and an aptamer-based PD-L1 probe was used for PD-L1 recognition. Due to the stable secondary structure of the designed connector, only cobinding of cholesterol-DNA and PD-L1 affinity probe induced biotin-labeled connector activation, while soluble PD-L1 cannot hybridize. As a result, PD-L1 EVs can be efficiently captured by streptavidin-functioned herringbone chip and quantified by anti-CD63-induced fluorescence signal. The high specificity of dual-input DNA computation allied to the high sensitivity of microfluidic-based detection was suitable for distinguishing lung cancer patients from healthy donors, highlighting its potential translation to clinical diagnosis and therapy monitoring.

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Programmable DNA-Fueled Electrochemical Analysis of Lung Cancer Exosomes

Cited by 32 publications

References 36 publications

Recent Advancement of PD-L1 Detection Technologies and Clinical Applications in the Era of Precision Cancer Therapy

Recent Advancement of PD-L1 Detection Technologies and Clinical Applications in the Era of Precision Cancer Therapy

Overview on the Design of Magnetically Assisted Electrochemical Biosensors

Reliable Detection of Extracellular PD-L1 by DNA Computation-Mediated Microfluidics

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