Prognostic value of matrix metalloproteinase-9 expression in oral squamous cell carcinoma and its association with angiogenesis

Abstract

BackgroundBreakdown of extracellular matrix (ECM) is one of the important hallmarks of cancer progression which facilitates the invasion of tumoral cells to the surrounding tissue. Matrix metalloproteinases (MMPs) can degrade various components of the ECM and basement membrane. The aim of this study was to determine the role of matrix metalloproteinases-9 protein in the biologic behavior of oral squamous cell carcinoma (OSCC) and its relation with tumor angiogenesis.Material and MethodsIn this study 42 OSCC and 15 normal epithelium were reviewed by immunohistochemical staining for matrix metalloproteinases-9 and CD105.ResultsMatrix metalloproteinases-9 expression was detected in 32 OSCC specimens (76.1%), with 28 specimens (66.6%) showing moderate or strong expression. We observed that the expression level of matrix metalloproteinases-9 was positively correlated with the status of lymph node metastasis (N0vs. N1) (P =0.00), and clinical stage (I-II vs. III-IV) in OSCC patients. Microvessel density in intratumoral tissue has an association with lymph node metastasis and advanced clinical stage (P=0.003 and p=0.01, respectively). We observed that tumors with matrix metalloproteinases-9 overexpression had a higher microvessel density counts compared with tumors with absent or focal immunostaining(16.2±5.6 vs 10.3±3.5 respectively, P =0.03).ConclusionsIn conclusion present results demonstrate the marked expression of matrix metalloproteinases-9 and CD105 in OSCC and suggest that the expression of these markers is associated with tumor progression and could offer additional information about the aggressiveness of OSCC. In addition a significant relationship was noted between microvessel density count and expression of matrix metalloproteinases-9 which suggest that MMP9 expression may be closely related to tumor angiogenesis. Key words:Matrix metalloproteinases-9, CD105, squamous cell carcinoma, immunohistochemistry.

Association between immunohistochemical expression of matrix metalloproteinases and metastasis in oral squamous cell carcinoma: Systematic review and meta‐analysis

“…Of these, 118 full‐text studies were assessed, of which 68 did not meet the inclusion criteria (Appendix 2). Therefore, 50 analytical cross‐sectional studies were included in the qualitative synthesis and 15 in the quantitative synthesis (Figure ).…”

“…Percentage of positive cells was the most used method, with further categorization using different cutoff values. Analyses were performed in tumor cells alone (tumor), tumor and stromal cells separately (tumor/stroma), and tumor and stroma simultaneously (tumor+stroma) . Detailed information regarding the main features, and findings of the included studies are presented in Table .…”

Ursolic acid induces apoptosis and autophagy in oral cancer cells

“…MMPs, a group of zinc-dependent endopeptidases, can degrade various components of the extracellular matrix and the basement membrane and is associated with tumor progression. 45 MMP-2 and MMP-9 over-activation are linked to poor survival in OSCC cases. 46,47 EGCG, the most well-known polyphenol in tea, inhibits the migration of human uveal melanoma cells by inhibiting MMP-2.…”

“…We found that the anti‐proliferative effect of UA on OSCC cells may be via the downregulation of ERK and p38 expression. MMPs, a group of zinc‐dependent endopeptidases, can degrade various components of the extracellular matrix and the basement membrane and is associated with tumor progression . MMP‐2 and MMP‐9 over‐activation are linked to poor survival in OSCC cases .…”

Osteopontin Promotes Bone Destruction in Periapical Periodontitis by Activating the NF-κB Pathway

“…Several studies have shown that LPS also directly induces osteoblast necrosis and apoptosis. In a study of fracture healing in an animal model, the injection of LPS delayed the healing of fractures and reduced the strength of the new bone [15][16][17].…”