Ursolic acid induces apoptosis and autophagy in oral cancer cells

Lin, Chia‐Der; Chin, Hong-Chan; Lee, Shou-Lun; Chiu, Chang‐Fang; Chung, Jing‐Gung; Lin, Ziyin; Wu, Chia-Yung; Liu, Ying-Chen; Hsiao, Yung-Ting; Feng, Chia‐Hsien; Bai, Li‐Yuan; Weng, Jing‐Ru

doi:10.1002/tox.22769

Cited by 48 publications

(37 citation statements)

References 50 publications

(105 reference statements)

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“…It also inhibits cell growth by inducing autophagy and apoptosis in human breast cancer cells T47D, MCF-7 and MD-MB-231 cells [279]. In contrast, UA induces autophagy, but the inhibition of autophagy enhances UA-induced apoptosis in human oral cancer Ca922 and SCC2095, and prostate cancer PC-3 cells [265,292]. Therefore, autophagy plays a dual role in UA-induced apoptosis via different signaling pathways.…”

Section: Ursolic Acid (Ua)mentioning

confidence: 99%

Naturally occurring anti-cancer compounds: shining from Chinese herbal medicine

et al. 2019

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Numerous natural products originated from Chinese herbal medicine exhibit anti-cancer activities, including antiproliferative, pro-apoptotic, anti-metastatic, anti-angiogenic effects, as well as regulate autophagy, reverse multidrug resistance, balance immunity, and enhance chemotherapy in vitro and in vivo. To provide new insights into the critical path ahead, we systemically reviewed the most recent advances (reported since 2011) on the key compounds with anti-cancer effects derived from Chinese herbal medicine (curcumin, epigallocatechin gallate, berberine, artemisinin, ginsenoside Rg3, ursolic acid, silibinin, emodin, triptolide, cucurbitacin B, tanshinone I, oridonin, shikonin, gambogic acid, artesunate, wogonin, β-elemene, and cepharanthine) in scientific databases (PubMed, Web of Science, Medline, Scopus, and Clinical Trials). With a broader perspective, we focused on their recently discovered and/or investigated pharmacological effects, novel mechanism of action, relevant clinical studies, and their innovative applications in combined therapy and immunomodulation. In addition, the present review has extended to describe other promising compounds including dihydroartemisinin, ginsenoside Rh2, compound K, cucurbitacins D, E, I, tanshinone IIA and cryptotanshinone in view of their potentials in cancer therapy. Up to now, the evidence about the immunomodulatory effects and clinical trials of natural anti-cancer compounds from Chinese herbal medicine is very limited, and further research is needed to monitor their immunoregulatory effects and explore their mechanisms of action as modulators of immune checkpoints.

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Section: Ursolic Acid (Ua)mentioning

confidence: 99%

Naturally occurring anti-cancer compounds: shining from Chinese herbal medicine

et al. 2019

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“…β-actin was used as the internal control. The expression of miRNAs was detected using a miRcute miRNA qPCR detection kit (SYBR Green; Tiangen Biotech Co., Ltd.) (13). Primer sequences are as follows: EGFR forward, 5'-CTA AGA TCC CGT CCA TCG CC-3' and reverse, 5'-GGA GCC CAG CAC TTT GAT CT-3'; cyclin D1 forward, 5'-GAT CAA GTG TGA CCC GGA C-3' and reverse, 5'-AGA GAT GGA AGG GGG AAA GA-3'; CDK4 forward, 5'-GCG TGA GGG TCT CCC TTG AT-3' and reverse, 5'-CAG TCG CCT CAG TAA AGC CA-3'; CDK6 forward, 5'-TCC CCA GAG TCT GAT TAC CT-3' and reverse, 5'-ACG ATT ACA TAG CCT CTG CC-3'; caspase 3 forward, 5'-ATG GAG AAC AAT AAA ACC T-3' and reverse, 5'-CTA GTG ATA AAA GTA GAG TTC-3'; Bcl-2 forward, 5'-CGA CGA CTT CTC CCG CCG CTA CC-3' and reverse, 5'-CCG CAT GCT GGG GCC GTA CAG-3'; Bax forward, 5'-TCC ACC AAG AAG CTG AGC GAG-3' and reverse, 5'-GTC CAG CCC ATG ATG GTT C-3'; matrix metallopeptidase 2 (MMP2) forward, 5'-CTA TTC TGC CAG CAC TTT GG-3' and reverse, 5'-CAG ACT TTG GTT CTC CAA C-3'; β-actin forward, 5'-GAG AGG GAA ATC GTG CGT GAC-3' and reverse, 5'-CAT CTG CTG GAA GGT GGA C-3' .…”

Section: Ursolic Acid Suppresses the Biological Function Of Osteosarcmentioning

confidence: 99%

Ursolic acid suppresses the biological function of osteosarcoma cells

et al. 2019

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Osteosarcoma is a highly malignant tumour that occurs in adolescents. Upregulation or the constitutive activation of epidermal growth factor receptor (EGFR) is a hallmark of osteosarcoma. To investigate the effect of ursolic acid on the biological function of osteosarcoma, MTT assay was used to detect the effect of ursolic acid on the proliferation of HOS and MG63 cells, while flow cytometry was used to analyse the effect on the cell cycle and apoptosis. Transwell and Matrigel assays were used to detect the effect of ursolic acid on cell migration and invasion, respectively. Western blot analysis and reverse transcription-quantitative polymerase chain reaction were used to detect the effects of different concentrations of ursolic acid on EGFR signaling pathway-related proteins, cell cycle, apoptosis and cell migration-related proteins. After overexpression or silencing of EGFR, the effects of ursolic acid on EGFR pathway and cell biological function were subsequently detected, using the same methods. The present study identified that ursolic acid had inhibitory effects on the growth and metastatic ability of osteosarcoma cells by suppressing EGFR.

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“…The compounds UA, UAD1, UAD2, UAD4, UAD5, UAD6, UAD7 and UAD8 showed In the present study, the cytotoxicity and the NF-κB expression were evaluated in A549 cells treated with semisynthetic UA derivatives, which showed a to induce apoptosis, being these effects associated with the inhibition of NF-κB [26]. In oral squamous cell carcinoma, the treatment with ursolic acid acted as a potent apoptosis inductor dependent of the caspase, being this mechanism regulated by the inhibition of the Akt/mTor/NF-κB signalling [27].…”

Section: Effect Of the Ursolic Acid Derivatives On Cell Viability Nfmentioning

confidence: 85%

Ursolic Acid Derivatives Induced Apoptosis and Reduces the NF-<i>κ</i>B in Human Lung Adenocarcinoma Cells

Scherrer¹,

Valadares²,

Alves³

et al. 2019

JCT

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Lung cancer is the major cause of death in the neoplastic diseases. In spite of the advances in the chemotherapy, the lung cancer treatments are still complex and costly, being necessary the seeking of new drugs. In this context, the ursolic acid (UA) becomes the target of studies that investigate its antitumor potential and, thus, structural modifications can enhance its biological activities. Eight UA semisynthetic derivative compounds (UAD1-8) were synthesized and evaluated their cytotoxic activity against human alveolar adenocarcinoma cells (A549). UAD1, UAD3, UAD5, UAD6 and UAD8 were able to reduce the viability of the A549 cells. Only UAD1 and UAD6 reduced the viability at 24 h, and only UAD3 didn't reduce the NF-κB expression. The compound UAD1 showed the greater apoptosis induction. Moreover, the compound UAD1 showed better results than UA in all assays. The present study shows, for the first time, the action of these compounds in the apoptotic effect, in the expression of NF-κB and in the A549 cell line. The ursolic acid derivatives showed substantial results in the apoptosis, cytotoxicity and NF-κB inhibition of A549 cells, and further studies are necessary for the development of possible new therapeutic drugs.

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Ursolic acid induces apoptosis and autophagy in oral cancer cells

Cited by 48 publications

References 50 publications

Naturally occurring anti-cancer compounds: shining from Chinese herbal medicine

Naturally occurring anti-cancer compounds: shining from Chinese herbal medicine

Ursolic acid suppresses the biological function of osteosarcoma cells

Ursolic Acid Derivatives Induced Apoptosis and Reduces the NF-<i>κ</i>B in Human Lung Adenocarcinoma Cells

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Ursolic acid induces apoptosis and autophagy in oral cancer cells

Cited by 48 publications

References 50 publications

Naturally occurring anti-cancer compounds: shining from Chinese herbal medicine

Naturally occurring anti-cancer compounds: shining from Chinese herbal medicine

Ursolic acid suppresses the biological function of osteosarcoma cells

Ursolic Acid Derivatives Induced Apoptosis and Reduces the NF-&lt;i&gt;κ&lt;/i&gt;B in Human Lung Adenocarcinoma Cells

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Ursolic Acid Derivatives Induced Apoptosis and Reduces the NF-<i>κ</i>B in Human Lung Adenocarcinoma Cells