Prognostic value of <i>p53</i> mutations, bax, and spontaneous apoptosis in maxillary sinus squamous cell carcinoma

Bandoh, Nobuyuki; Hayashi, Tatsuya; Kishibe, Kan; Takahara, Miki; Imada, Masanobu; Nonaka, Satoshi; Harabuchi, Yasuaki

doi:10.1002/cncr.10388

Cited by 69 publications

(53 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several pathways mediated p53-induced apoptosis, and one of these involved the bax gene that was the p53 target and the proapoptotic member of the Bcl-2 family of genes. [45][46][47] Bax could promote the cytosolic release of cytochrome c, which in turn, activated caspase 3, one of the key executioners of apoptosis, and then apoptosis occurred. 48,49) In addition, bax could bind with bcl-2 and inhibit its function of apoptosis suppression.…”

Section: Discussionmentioning

confidence: 99%

The Anticancer Activities of Wogonin in Murine Sarcoma S180 both in Vitro and in Vivo

Wang

Guo

Qin

et al. 2006

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

The anticancer effects of wogonin on murine sarcoma S180 both in vitro and in vivo were investigated, and its pro-apoptotic molecular mechanism was further studied. Wogonin treatment resulted in significant inhibition of S180 cells in a concentration-dependent manner detected by MTT assay. The IC 50 value for 48 h was (7.37؎1.53)؋10 ؊5 M. Typical morphological changes and apoptosis bleb phenomenon in S180 cells exposed to wogonin were distinctly observed by the inverted light microscope and the fluorescence microscope, respectively. According to protocols of transplanted tumor research, 1) mice were transplanted with tumor cells S180. The weight of tumor and the peripheral leucocyte count were observed after the treatment of wogonin. The significant suppression of tumor growth was observed, and the peripheral leucocyte count of S180-bearing mice remained no significant changes compared with control group. After the treatment of 40 mg/kg wogonin, the inhibitory rate of tumor weight was 53.01%. Additional DNA fragmentation assay showed that wogonin induced apoptosis on murine sarcoma S180 tissue. RT-PCR results indicated that the increasing mRNA levels of bax and p53 and the decreasing mRNA level of bcl-2 were induced by wogonin. Western-blot assay showed that the increasing protein level of bax and the decreasing protein level of bcl-2 were induced by wogonin. Collectively, wogonin could induce apoptosis in murine sarcoma S180 thereby inhibiting the tumor growth both in vitro and in vivo. The pro-apoptotic effects might be related to the improvement of mRNA level of p53, the improvement of mRNA and protein levels of bax, and the reduction of mRNA and protein levels of bcl-2.

show abstract

Section: Discussionmentioning

confidence: 99%

The Anticancer Activities of Wogonin in Murine Sarcoma S180 both in Vitro and in Vivo

Wang

Guo

Qin

et al. 2006

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

“…Goat anti-human CXCR4 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA) at 1:200 dilution and mouse antihuman SDF-1 antibody (25 Ag/mL, GT, Minneapolis, MN) were used. These primary antibodies were incubated overnight at 4jC followed by incubation with peroxidase-labeled dextran polymers, Simple Stain MAX (Nichirei, Tokyo, Japan) for CXCR4 stain and EnVision+ (DAKO A/S, Carpinteria, CA) for SDF-1 stain, for 30 minutes at room temperature (25). They were visualized by immersing the slides in freshly prepared 0.02% 3,3V-diaminobenzidine solution for 10 minutes.…”

Section: Methodsmentioning

confidence: 99%

Expression of CXCR4 and Its Down-Regulation by IFN-γ in Head and Neck Squamous Cell Carcinoma

Katayama

Ogino

Bandoh

et al. 2005

Clinical Cancer Research

Self Cite

View full text Add to dashboard Cite

Purpose: The functional expression of CXCR4, which plays roles in cell migration and proliferation in response to its unique ligand stromal cell^derived factor-1 (SDF-1), has been reported in variety of carcinomas. However, CXCR4 expression and its functional role in head and neck squamous cell carcinomas (HNSCC) remain unclear. In this study, we investigated CXCR4 expression and analyzed its functions in HNSCC cell lines. We also attempted to regulate CXCR4 expression using cytokines, such as interleukin-1B , tumor necrosis factor-A, and IFN-;. Finally, we investigated correlation between CXCR4 expression and clinical features in patients with HNSCC. Experimental Design: Six HNSCC cell lines were used in this study. Reverse transcription-PCR and flow cytometry analysis were shown for CXCR4 expressions with or without stimulations of cytokines. SDF-1-mediated cell migration was assayed in Matrigel-coated chemotaxis chamber. The SDF-1-mediated cell proliferation was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The SDF-1-mediated signaling pathways were analyzed by Western blot analysis. Biopsy specimens from 56 patients with HNSCC were used for immunohistologic analysis. Results: The significant CXCR4 expression was found in HSQ-89, IMC-3, and Nakamura cells. The SDF-1-mediated cell migration and proliferation were observed in CXCR4-positive cells. SDF-1 also promoted rapid phosphorylation of extracellular signal-regulated kinase 1/2 and Akt signaling pathways in CXCR4-positive cells. The SDF-1-mediated cell migration and proliferation of CXCR4-positive cells were inhibited by neutralization of CXCR4. Among three cytokines tested, IFN-; significantly reduced CXCR4 expression and SDF-1-induced cell migration and proliferation of CXCR4-positive cells. Immunohistologic analysis revealed that patients with advanced neck status and patients who developed distant metastases showed significantly higher CXCR4 expression, and the cause-specific survival of patients with CXCR4-expression was significantly shorter. Furthermore, multivariate analysis confirmed that CXCR4 positive was the independent factor for cause-specific death. Conclusion: Our results may provide an insight into future therapeutic agent that inhibits tumor metastasis and progression via down-regulating CXCR4 expression in patients with HNSCC.

show abstract

“…However, head and neck squamous cell carcinoma (SCC), thyroid carcinoma (TC), and thoracoabdominal malignancies often metastasize to the CLN 1, 2. Furthermore, malignant lymphoma (ML) sometimes shows CLN enlargement as the first symptom.…”

mentioning

confidence: 99%

Diagnostic value of liquid‐based cytology with fine needle aspiration specimens for cervical lymphadenopathy

Bandoh

Goto

Akahane

et al. 2016

Diagnostic Cytopathology

Self Cite

View full text Add to dashboard Cite

BackgroundCervical lymphadenopathy is a symptom that is frequently seen among outpatients, and it is important to differentiate malignant lesions from reactive lymphoid hyperplasia. Fine needle aspiration (FNA) cytology has been widely used for the diagnosis of cervical lymphadenopathy. However, some limitations of the diagnostic accuracy using conventional smear (CS) cytology have been pointed out. The diagnostic value of liquid‐based cytology (LBC) with FNA specimens has not yet been fully proven.MethodsForty‐two patients with cervical lymphadenopathy who underwent FNA with CS cytology from 2007 to 2011 and 123 patients who underwent FNA with LBC utilizing LBCPREP2™ from 2011 to 2015 were studied. Diagnostic values were compared between the CS and the LBC groups.ResultsOf the total 165 patients representing the combined CS and LBC groups, 81 (49.1%) were diagnosed as benign lymph node and 84 (50.9%) were malignant diseases including 37 (22.4%) of metastatic carcinoma except for thyroid carcinoma, 30 (18.2%) of metastatic thyroid carcinoma, and 17 (10.3%) of malignant lymphoma. The overall statistical values including sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the CS were 75%, 100%, 100%, 78.9%, and 87.1%, respectively, whereas those values for LBC were 91.2%, 100%, 100%, 90.7%, and 95.3%, respectively. The sensitivity of LBC for malignant diseases tended to be higher than that of CS cytology (p = 0.081).ConclusionLBC with FNA specimens from cervical lymphadenopathy is a useful and reliable method for the diagnosis of malignant diseases, especially of metastatic carcinomas, due to its increased sensitivity compared with CS cytology. Diagn. Cytopathol. 2016;44:169–176. © 2016 Wiley Periodicals, Inc.

show abstract

Prognostic value of p53 mutations, bax, and spontaneous apoptosis in maxillary sinus squamous cell carcinoma

Abstract: 出版社

Cited by 69 publications

References 54 publications

The Anticancer Activities of Wogonin in Murine Sarcoma S180 both in Vitro and in Vivo

The Anticancer Activities of Wogonin in Murine Sarcoma S180 both in Vitro and in Vivo

Expression of CXCR4 and Its Down-Regulation by IFN-γ in Head and Neck Squamous Cell Carcinoma

Diagnostic value of liquid‐based cytology with fine needle aspiration specimens for cervical lymphadenopathy

Contact Info

Product

Resources

About