Prognostic value of human papillomavirus 16/18 genotyping in low-grade cervical lesions preceded by mildly abnormal cytology

Ye, Jing; Cheng, Bei; Cheng, Yifan; Yao, Yeli; Xie, Xing; Lü, Weiguo; Cheng, Xiaodong

doi:10.1631/jzus.b1600473

Cited by 13 publications

(8 citation statements)

References 37 publications

(45 reference statements)

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“…Some studies reported that genotyping techniques might be useful to improve the triage and follow up of HPV infected women [17]. In fact, some authors suggested that the genotypes 16/18 are related to a more elevated progressive risk [18,19].…”

Section: Discussionmentioning

confidence: 99%

A Retrospective Study about the Impact of Switching from Nested PCR to Multiplex Real-Time PCR on the Distribution of the Human Papillomavirus (HPV) Genotypes

et al. 2019

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Background and objectives: Human papillomavirus (HPV) is the most prevalent etiological agent of viral sexually-transmitted infection. This study retrospectively evaluated the impact of a switch to a real-time PCR assay in the HPV prevalence and genotypes distribution by a quasi-experimental before-and-after approach. Materials and Methods: In total, 1742 samples collected from 1433 patients were analyzed at the UOC Microbiology and Virology of Policlinico of Bari, Italy. HPV DNA detection was performed using initially nested PCR and subsequently multiplex real-time PCR assay. Results: Statistically significant difference in HPV overall prevalence after the introduction of the real-time assay was not detected (48.97% vs. 50.62%). According to different extraction-DNA amplification methods, differences were observed in the prevalence rates of HPV-45, 68, 40, 42, and 43. The lowest prevalence for HPV-45 was observed in the Magna Pure-Real Time PCR group, while HPV-68, 40, 42, and 43 were less observed in the Qiagen-Real Time PCR group. After, a multivariate logistic regression, an increase in the prevalence of HPV-42 (aOR: 4.08, 95% CI: 1.71–9.73) was associated with the multiplex real-time PCR assay. Conclusions: Although this study is a not a direct comparison between two diagnostic methods because it has a sequential structure, it serves to verify the impact of a new molecular assay on HPV distribution. Moreover, the stability of HPV prevalence over time suggests that the population composition and the behavioral variables did not likely change during the observation period. Our study proposes that the introduction of a molecular test for HPV detection may be related to changes of HPV genotypes distribution.

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Section: Discussionmentioning

confidence: 99%

A Retrospective Study about the Impact of Switching from Nested PCR to Multiplex Real-Time PCR on the Distribution of the Human Papillomavirus (HPV) Genotypes

et al. 2019

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“…The risk for developing low or high-grade cervical lesions originating from cytological abnormalities increases with age 31 ; therefore, HPV detection is recommended in women older than 30 years. However, the average onset of sexual activity in Mexico is at the age of 16 32 , and considering that HPV transformation processes can take 5-10 years, there is a possibility that some young women, under 30 years, may develop premalignant lesions or cancer without falling into the group of women older than 30 in whom HPV detection is recommended.…”

Section: Hr-hpv Dna Detection Tests As Primary Screening For Cervical Cancermentioning

confidence: 99%

“…The main RNA detection techniques for E6 and E7 viral oncogenes include the PreTect® HPV-Proofer and the APTIMA® tests. The PreTect® HPV-Proofer test allows the detection of five types of HR HPV 16,18,31,33,45 with high sensitivity for the detection of high-grade squamous intraepithelial lesions (HSIL); however, it has not been approved by the FDA for use in CC screening 37 .…”

Section: Detection Of E6 and E7 Oncogenes Messenger Rnasmentioning

confidence: 99%

Molecular Markers for the Diagnosis of High-Risk Human Papillomavirus Infection and Triage of Human Papillomavirus-Positive Women

Torres-Poveda

Piña‐Sánchez

Vallejo-Ruíz

et al. 2020

RIC

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Infection with high-risk human papillomavirus (HPV) increases the likelihood of developing cervical cancer (CC). A plethora of cellular processes is required to produce pre-malignant lesions, which in turn may become malignant if left untreated. Those changes are induced by viral oncoproteins, which represent an ideal target to identify the viral presence, or by some particularities of the host that ultimately promote the establishment of CC. This article describes the different methods used for HPV detection and quantification, as well as the current trend of secondary screening approaches to detect premalignant lesions and CC. In addition, we analyzed validated biomarkers and those under clinical investigation for the classification (triage) of women at risk of developing CC after an initial positive HPV test and that could be used as prognostic biomarkers for CC. The use of molecular biomarkers, together with the detection of HPV DNA sequences, provides a high impact diagnostic and prognostic tool in the detection of patients at increased risk of developing CC and also may guide their clinical management. In addition, some of those biomarkers could represent pharmacological targets for the future design of therapeutic approaches to CC treatment.

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“…Low-grade squamous intraepithelial lesion (LSIL) presents productive viral infection and corresponding clinical measures will be in place. Around 70–80% LSIL are able to regress spontaneously within 1–2 years, but 30% LSIL are refractory and 10% LSIL patients will develop high grade SIL (HSIL) that could evolve into a malignancy [ 5 ]. According to cervical carcinoma screening guideline of NCCN, clinical observations are recommended for treatment of LSIL, accompanied with close follow-up Pap tests and colposcopic examinations until the lesions resolve.…”

Section: Introductionmentioning

confidence: 99%

Prognostic value of HPV 16/18 genotyping and geminin mRNA quantification in low-grade cervical squamous intraepithelial lesion

et al. 2021

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Low-grade cervical squamous intraepithelial lesion is a precancerous neoplasia that has appreciable probability to evolve into malignancy. To explore the prognostic value of HPV 16/18 genotyping and geminin mRNA quantification in predicting the progressiveness of LSIL. We recruited 212 participants who were negative for intraepithelial lesion or malignancy (NILM 76), low-grade squamous intraepithelial lesion (LSIL 85), high-grade squamous intraepithelial lesion (HSIL 36) and cervical intraepithelial neoplasia grade cervical cancer grade 3, (CIN3 15) patients. Tissues were obtained during excisional treatment. HPV 16/18 genotyping and geminin mRNA qRT-PCR were performed. HPV 16/18 positivity rate and geminin mRNA level were integrated with the clinical parameters into a multivariate logistic model. Area under curve was yielded based on receiver operation curve derived from this multivariate logistic model. Follow-up visits were performed to LSIL patients with progression. HSIL patients have higher HPV 16/18 positivity rate and geminin mRNA levels than LSIL. Among HSIL, CIN3 have higher HPV 16/18 positivity rate and geminin mRNA levels. Multivariate logistic analysis showed that HPV 16/18 positivity and geminin mRNA expression status are independent factors for differentiating HSIL and LSIL. The baseline HPV 16/18 positivity rate and geminin mRNA levels of 18 LSIL patients who developed HSIL are significantly higher than non-progressive LSIL patients. The values examined at follow-up timepoints were also higher than baseline. These results suggest that geminin is implicated in the progression of LSIL and combining HPV 16/18 genotyping and geminin mRNA qRT-PCR could potentially differentiating the progressive LSIL and improve the efficacy of clinical intervention.

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Prognostic value of human papillomavirus 16/18 genotyping in low-grade cervical lesions preceded by mildly abnormal cytology

Cited by 13 publications

References 37 publications

A Retrospective Study about the Impact of Switching from Nested PCR to Multiplex Real-Time PCR on the Distribution of the Human Papillomavirus (HPV) Genotypes

A Retrospective Study about the Impact of Switching from Nested PCR to Multiplex Real-Time PCR on the Distribution of the Human Papillomavirus (HPV) Genotypes

Molecular Markers for the Diagnosis of High-Risk Human Papillomavirus Infection and Triage of Human Papillomavirus-Positive Women

Prognostic value of HPV 16/18 genotyping and geminin mRNA quantification in low-grade cervical squamous intraepithelial lesion

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