Prognostic value of HPV 16/18 genotyping and geminin mRNA quantification in low-grade cervical squamous intraepithelial lesion

Abstract

Low-grade cervical squamous intraepithelial lesion is a precancerous neoplasia that has appreciable probability to evolve into malignancy. To explore the prognostic value of HPV 16/18 genotyping and geminin mRNA quantification in predicting the progressiveness of LSIL. We recruited 212 participants who were negative for intraepithelial lesion or malignancy (NILM 76), low-grade squamous intraepithelial lesion (LSIL 85), high-grade squamous intraepithelial lesion (HSIL 36) and cervical intraepithelial neoplasia grade cervical cancer grade 3, (CIN3 15) patients. Tissues were obtained during excisional treatment. HPV 16/18 genotyping and geminin mRNA qRT-PCR were performed. HPV 16/18 positivity rate and geminin mRNA level were integrated with the clinical parameters into a multivariate logistic model. Area under curve was yielded based on receiver operation curve derived from this multivariate logistic model. Follow-up visits were performed to LSIL patients with progression. HSIL patients have higher HPV 16/18 positivity rate and geminin mRNA levels than LSIL. Among HSIL, CIN3 have higher HPV 16/18 positivity rate and geminin mRNA levels. Multivariate logistic analysis showed that HPV 16/18 positivity and geminin mRNA expression status are independent factors for differentiating HSIL and LSIL. The baseline HPV 16/18 positivity rate and geminin mRNA levels of 18 LSIL patients who developed HSIL are significantly higher than non-progressive LSIL patients. The values examined at follow-up timepoints were also higher than baseline. These results suggest that geminin is implicated in the progression of LSIL and combining HPV 16/18 genotyping and geminin mRNA qRT-PCR could potentially differentiating the progressive LSIL and improve the efficacy of clinical intervention.

Role of Geminin as a Tool for Augmenting Accurate Diagnosis of Cervical Neoplasia

“…However, more women with CIN3 or CC had geminin positivity staining as compared to those with CIN1 or CIN2 (Table 2 ), and the correlation was significantly higher for CC compared to CIN1 or CIN2 (Table 3 ). Zheng et al [ 16 ] also found HPV 16/18 positivity status, expression of geminin, presence of squamocolumnar junction, and size of lesion as independent predictors for differentiating between low-grade squamous intraepithelial lesions and high-grade squamous intraepithelial lesions. They reported HPV 16/18 status and geminin as the most significant associations.…”

“…They reported a sensitivity of 95%, a positive predictive value of 85.7%, and an accuracy of 88.2%. Geminin levels were found to increase with the grade of the lesion and also change during the progression of LSIL/CIN1 to HSIL/CIN2-3 [ 16 ].…”

Gym-based aerobic exercise with bicycle ergometer contributes to the regression of human papilloma virus infection: a single-center, single-blind and randomized controlled trial

“…HR-HPV is an indispensable precursor of CC, whereas LR-HPV is integral for genital mucosal warts. In particular, HR-HPV 16/18 or non-16/18 HR-HPV are considered oncogenic and causative [4][5][6]. Although occasionally eliminated by immune processes, HR-HPV is now progressively difficult to eliminate due to the influence of smoking, alcohol consumption or irregular sexual activity.…”