Prognostic value of DNA quantification in early epithelial ovarian carcinoma

Abstract

Flow cytometric DNA quantification was the main independent prognostic factor of relapse and survival in these women with stages I-II epithelial ovarian cancer.

Correlation Between MIB1-Determined Tumor Growth Fraction and Incidence of Tumor Recurrence in Early Ovarian Carcinomas

“…Cyst rupture [40 -42] and delayed laparotomy after laparoscopic removal of ovarian masses later found malignant, [43] as well as deoxyribo nucleic acid (DNA) ploidy [28,44,45] or combined scores of different biological markers such as DNA content, MIB1, p53, and WAF-1 [39] may have therapeutic consequences. Cyst rupture [40 -42] and delayed laparotomy after laparoscopic removal of ovarian masses later found malignant, [43] as well as deoxyribo nucleic acid (DNA) ploidy [28,44,45] or combined scores of different biological markers such as DNA content, MIB1, p53, and WAF-1 [39] may have therapeutic consequences.…”

“…[24,25] Tumors were classified as transitional-cell carcinoma when exhibiting more than 50% of corresponding patterns [26] and uniform tumor grading was performed. [28] Immunohistochemistry was applied on 2 -3-mm-thick sections of representative tumor areas, including two or more specimens per case, if necessary, due to variations in tumor differentiation.…”

Comparison of clinical staging of benign and malignant ovarian tissues with DNA flow cytometry

“…This finding is well supported in the literature [39,40,50,52,64] . The presence of this atypical DNA suggests a prognosis that is poorer than a sample with normal DNA [6,8,11,13,14,16,24,39,41,43,52,59,60,66,70] . The aneuploid characterization of these benign tumors should at least require a closer, independent pathological examination.…”

“…Valverde et al [59] in a study of 77 cases of stage I-II ovarian cancer after comprehensive surgical staging showed that flow cytometric DNA quantification was the main independent prognostic factor of relapse and survival in these women. Pfisterer et al [60] measured DNA content and S-phase fraction, with flow cytometry, of tumor specimens from 162 women with no pretreated surgically staged FIGO stage I endometrial cancer using clearly defined inclusion criteria.…”

BRCA1 mutation site may be linked with nuclear DNA ploidy in BRCA1‐mutated ovarian carcinomas

“…This is consistent with a previous study by Valverde et al . who found no association between DNA ploidy and p53 . The other possible mechanism for DNA aneuploidy in sporadic ovarian cancers is BRCA1 inactivation through mechanisms other than mutation such as methylation .…”