Prognostic Significance of Oxidative Stress Markers in Colitis Associated with Phenylbutazone Administration in Draft Horses

El-Ashker, Maged; El-Khodery, Sabry; Metwally, Nadia S.; Hussein, Hussein; El‐Boshy, Mohamed

doi:10.1016/j.jevs.2011.08.008

Cited by 11 publications

(19 citation statements)

References 33 publications

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“…Similar findings were reported in gastric tissue of rats treated with indomethacin (Motawi et al, 2008;Abdallah, 2010). However, El-Ashker et al (2012) found high prognostic value of NO in colitis associated with PBZ administration, significantly increasing in non-surviving patients.…”

Section: Discussionsupporting

confidence: 84%

“…The combination of both factors is usually underestimated. The clinical and laboratory effects found in this study were similar to those reported under experimental conditions and case reports of colitis associated to PBZ administration (El-Ashker et al, 2012). However, variations of these results were not analyzed for statistical significance; additionally, horse 1 died and its data were not included in the analysis.…”

Section: Discussionsupporting

confidence: 82%

“…Another study also showed increased PGE 2 concentration in gastric contents after oral administration of PBZ (Martínez-Aranzales et al, 2014). The findings support the hypothesis that oxidative stress is another pathway in gastrointestinal mucosal injury; for instance, NO and MDA concentrations have been experimentally observed in gastric tissue after a single administration of PBZ (Martínez-Aranzales et al, 2014), and plasma concentration of those metabolites has been reported in clinical cases (El-Ashker et al, 2012).…”

Section: Discussionsupporting

confidence: 62%

“…Hypoproteinemia and hypoalbuminemia are also considered sensitive biochemical indicators of PBZ-induced enteropathy in the horse (El-Ashker et al, 2012). Loss of plasma proteins through the gastrointestinal tract is the result of ulcerations and bleeding, evidenced by the presence of FOB and initial thrombocytopenia, as shown in this assay.…”

Section: Discussionmentioning

confidence: 90%

“…This finding is characteristic in horses treated with PBZ or horses with severe endotoxemia (McConnico et al, 2008;El-Ashker et al, 2012). The role of infiltrating neutrophils in damaged intestine is well known (Gayle et al, 2000;Tomlinson and Wilder, 2004); however, there is evidence of response variability in segments of the digestive tract of horses in accordance with the nonsteroidal anti-inflammatory drug (NSAID) type (Marshall and Blikslager, 2011).…”

Section: Discussionmentioning

confidence: 99%

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Nitric oxide and malondialdehyde in gastric contents and blood in an equine model of gastric ulcer induced by phenylbutazone

Zuluaga

2016

Rev Colomb Cienc Pecu

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Concentración de óxido nítrico y malonaldehído en contenido gástrico y en sangre en un modelo de úlcera gástrica equina inducida por fenilbutazona Concentração de óxido nítrico e malondialdeído no conteúdo gástrico e no sangue em modelo equino de úlcera gástrica Summary Background: mechanisms of gastric mucosal injury include cellular damage by oxygen free radicals, which can be indirectly measured through malondialdehyde (MDA). Production of nitric oxide (NO) maintains gastric tissue perfusion through vasodilatation. Objective: to demonstrate oxidative stress and impaired gastric perfusion measuring NO and MDA in gastric contents and blood of equines subjected to a gastrointestinal ulceration induction protocol. Methods: a gastrointestinal ulceration induction protocol involving fasting and oral administration of phenylbutazone was performed on five horses. NO and MDA were measured before and after protocol induction and presence of fecal occult blood (FOB) was evaluated. Animals underwent gastroscopy at the beginning and end of the protocol. Results: horses presented variability in hematological and FOB exams. Azotemia, hyperphosphatemia, and hypocalcemia were found in all animals. No significant changes were found in enzymatic activity. At the end of the protocol, 40% of the horses showed varying degrees of gastric ulceration. NO production in the stomach decreased by 60%, whereas MDA production increased by 55% after the protocol. Plasma concentration of MDA average increased 96 hours after starting the protocol. There were no significant differences in mean plasma NO during the protocol. Conclusion: the

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Section: Discussionsupporting

confidence: 84%

Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

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Nitric oxide and malondialdehyde in gastric contents and blood in an equine model of gastric ulcer induced by phenylbutazone

Zuluaga

2016

Rev Colomb Cienc Pecu

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Evaluation of gastrointestinal tract lesions and serum malondialdehyde levels after repeated oral administration of phenylbutazone in horses

Tesena,

Vinijkumthorn,

Preuksathaporn

et al. 2024

Vet Res Commun

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Phenylbutazone (PBZ) is a widely used nonsteroidal anti-inflammatory drug for horses. However, because of its gastrointestinal side effects, its administration requires careful attention in veterinary practice. Malondialdehyde (MDA) is a serum biomarker associated with increased damage to the equine gastrointestinal system. This study investigated the hematological effects and alterations in the gastrointestinal tract and assessed serum MDA concentrations following repeated oral PBZ administration at clinical doses. Fourteen horses were randomly divided into control and treatment groups. All horses in the treatment group were administered 4.4 milligrams per kilogram of body weight of PBZ syrup orally twice a day for 7 days, whereas the control group received syrup as a placebo. The development of gastrointestinal side effects was investigated using gastroscopy, abdominal ultrasound, and fecal pH; serum MDA concentrations were assessed using a commercially available enzyme-linked immunosorbent assay kit. Data were compared between PBZ-treated and control horses before and after the treatment period. The treatment group exhibited decreased albumin and total protein concentrations. Moreover, this group exhibited a higher thickness of the right dorsal colon wall (p = 0.03) and had higher scores for squamous gastric ulcers (p = 0.01). Fecal pH was lower in the treatment group than in the control group after PBZ administration (p < 0.01). Although MDA concentrations were higher in the treatment group after PBZ administration, they did not differ significantly from those of the control group. This study highlighted the changes in hematological and gastrointestinal lesions resulting from PBZ administration in horses at clinical doses, even without clinical signs. However, MDA may not be an optimal biomarker for the early detection of gastrointestinal damage due to PBZ treatment in horses.

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The Contribution of Specific and Nonspecific Biomarkers in Diagnosis of Equine Gastric Ulcer Syndrome (EGUS) Under Field Condition

Shawaf

El-Deeb

Elgioushy

2020

Journal of Equine Veterinary Science

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Prognostic Significance of Oxidative Stress Markers in Colitis Associated with Phenylbutazone Administration in Draft Horses

Cited by 11 publications

References 33 publications

Nitric oxide and malondialdehyde in gastric contents and blood in an equine model of gastric ulcer induced by phenylbutazone

Nitric oxide and malondialdehyde in gastric contents and blood in an equine model of gastric ulcer induced by phenylbutazone

Evaluation of gastrointestinal tract lesions and serum malondialdehyde levels after repeated oral administration of phenylbutazone in horses

The Contribution of Specific and Nonspecific Biomarkers in Diagnosis of Equine Gastric Ulcer Syndrome (EGUS) Under Field Condition

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