Prognostic significance of hsa_circ_0048122 to predict liver metastasis in early‐stage colorectal cancer

Abstract

Background: Liver metastasis is the primary cause of lethal colorectal cancer (CRC).The predominant risk of poor patient prognosis in early-stage CRC emerges as metachronous liver metastasis. This necessitates the search for potential biomarkers for this metastasis to assess treatment outcomes and provide targeted therapy. Methods:The role of hsa_circ_0048122 in predicting liver metastasis in CRC was probed in this work. This retrospective and multi-center investigation entailed exploration and identification stages with 158 and 176 patients. While RT-qPCR was employed to scrutinize hsa_circ_0048122 expression, Kaplan-Meier survival, and multivariate analyses were used to probe its prognostic impact in early-stage CRC and stage IV CRC cases, respectively.Results: A strong correlation between liver metastases and hsa_circ_0048122 expression in stage IV CRC patients with a high hsa_circ_0048122 profile indicated a poor overall survival. Likewise, a high expression level of hsa_circ_0048122 appears as a potential predictor of liver metastases in patients' initial stages. Conclusions:Predicting liver metastasis can be plausibly facilitated using Hsa_ circ_0048122 as a biomarker in early-stage CRC cases.

Cirscan: a shiny application to identify differentially active sponge mechanisms and visualize circRNA–miRNA–mRNA networks

“…4 D). Among the 10 ce-circRNA candidates, 0048122 is identified as being involved in colorectal cancer [ 25 ] and 0000644 in bladder cancer [ 58 ]. The other best ce-circRNA candidates ( 0000516, 0087643, 0052523, 0046599, 0042521, 0064288) have not yet been described in the literature as associated with cancer, making them interisting subjects for further experimental validations.…”

“…We evaluated Cirscan on public multi-level transcript expression dataset from two cancers: colorectal cancer [ 24 ] and hepatocarcinoma cancer [ 25 ] and showed that Cirscan was able to retrieve previously described sponge mechanisms in the top ranked circRNA–miRNA–mRNA subnetworks, as well as identifying novel circRNA sponge candidates.…”

Cirscan: a shiny application to identify differentially active sponge mechanisms and visualize circRNA-miRNA-mRNA networks

“…Cirscan identified a potential novel sponge mechanisms for the hsa circ 0000517, involving the hsa-miR-330-5p that targets genes, for example the oncogene NRAS, enriched in different cancers, such as hepatocellular carcinoma and bladder cancer (Figure 4.D). Among the 10 ce-circRNA candidates, hsa circ 0048122 is identified as being involved in colorectal cancer [25] and hsa circ 0000644 in bladder cancer [53]. The other best ce-circRNA candidates (hsa circ 0000516, hsa circ 0087643, hsa circ 0052523, hsa circ 0046599, hsa circ 0042521, hsa circ 0064288) have not yet been described in the literature as associated with cancer, making them interisting subjects for further experimental validations.…”

“…In addition, our multiple criteria strategy leads to the calculation of a sponge score for each circRNA-miRNA-mRNA subnetwork, that greatly facilitates the prioritization of experimental validations. We evaluated Cirscan on public multi-level transcript expression dataset from two cancers: colorectal cancer [24] and hepatocarcinoma cancer [25] and showed that Cirscan was able to retrieve previously described sponge mechanisms in the top ranked circRNA-miRNA-mRNA subnetworks, as well as identifying novel circRNA sponge candidates.…”