Prognostic significance of DNA ploidy and proliferative index (MIB-1 index) in gastrointestinal stromal tumors

Carrillo, Rosario; Candia, Antonio; Rodríguez-Peralto, José L.; Caz, Víctor

doi:10.1016/s0046-8177(97)90100-3

Cited by 110 publications

(91 citation statements)

References 38 publications

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“…In contrast, Sbasching et al [27] did not find a correlation of PCNA expression with survival in GIST. More recently, Ki-67 analogues have replaced PCNA because PCNA index is instable due to variations in antigen preservation and recovery [29] . Seidal et al [30] studied 31 cases of GIST and found a correlation with Ki-67 and outcome of patients.…”

Section: Figurementioning

confidence: 99%

Expression of COX-2, PCNA, Ki-67 and p53 in gastrointestinal stromal tumors and its relationship with histopathological parameters

Gümürdülü

Erdoğan²,

Kayaselçuk³

et al. 2007

WJG

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AIM:To investigate the expression of Cyclooxygenase-2 (COX-2), proliferating cell nuclear antigen (PCNA), Ki-67 and p53 in gastrointestinal stromal tumors (GISTs) and its relationship with histopathological parameters. METHODS:Twenty-five GISTs were examined by light microscopy and immunohistochemistry. c-kit, CD34, SMA, S-100 protein, COX-2, PCNA, Ki-67 and p53 were detected immunohistochemically and the relationship was evaluated among histopathologic parameters such as mitotic index (MI), tumor grade, tumor size, COX-2, PCNA, Ki-67 and p53.RESULTS: COX-2 protein expression was found in 19 of 25 (76%) of the tumors, and expression was noted in the cytoplasm of the tumor cells. p53 was significantly related to MI and tumor grade but no relationship was found between COX-2, proliferation markers and MI, tumor grade and tumor size. CONCLUSION: COX-2 is expressed in most GISTsand it may play an important role in the proliferation and progression of these tumors or a useful marker to identify GIST. Although immunohistochemical assessment of p53 can be used for distinguishing the risk groups of GISTs, tumor size and mitotic rate should be considered at the same time.

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Section: Figurementioning

confidence: 99%

Expression of COX-2, PCNA, Ki-67 and p53 in gastrointestinal stromal tumors and its relationship with histopathological parameters

Gümürdülü

Erdoğan²,

Kayaselçuk³

et al. 2007

WJG

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show abstract

“…Although the most important and easily applicable criteria for predicting the tumor behavior are tumor size and mitotic activity (2)(3)(4), small portions of tumors apparently lacking mitotic activity have been found to metastasize. Several studies have identified additional prognostic factors, including immunohistochemical markers of cell proliferation (e.g., Ki-67, PCNA) and DNA flow cytometry (5,6), but none of these has shown to be consistently relevant compared with the conventional morphological criteria.…”

Section: Introductionmentioning

confidence: 99%

Prognostic Significance of c-kit Mutation in Localized Gastrointestinal Stromal Tumors

Kim¹,

Lee²,

Kang³

et al. 2004

Clinical Cancer Research

145

102

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Purpose: Constitutive mutational activation of c-kit has been found to be associated with the pathogenesis of gastrointestinal stromal tumors (GISTs). The prognostic significance of c-kit mutations, however, is still controversial.Experimental Design: We examined 86 patients curatively resected for localized GIST. Genomic DNA was extracted from paraffin-embedded tumor tissues. Exons 9, 11, 13, and 17 of the c-kit gene were amplified by PCR and sequenced.Results: Mutations in exon 11 were detected in 61 tumors, and mutations in exon 9 were observed in three tumors, whereas no mutations were detected in exons 13 or 17. The overall c-kit mutation frequency was 74%. Amino acid alterations in the 61 tumors with exon 11 mutations were deletion in 33 tumors, substitution in 20, both deletion and substitution in 4, insertion in 1, and duplication in 3. Histologically, tumors with c-kit mutations showed higher mitotic counts and higher cellularity. The 5-year relapse-free survival (RFS) in patients having GISTs with c-kit mutations was 21%, compared with 60% in those without c-kit mutations. Significantly higher RFS rates were observed in patients with tumors having mitotic counts < 5 mitoses/50 high power field, spindle-cell histology, tumor size < 5 cm, or gastric GISTs. Multivariate analyses indicated association of poorer RFS with a higher mitotic count [>5 of 50 high power fields; odds ratio (OR) ‫؍‬ 3.0], presence of c-kit mutations (OR ‫؍‬ 5.6), and a larger tumor size (>5 cm; OR ‫؍‬ 4.2).Conclusions: The presence of c-kit mutation, along with high mitotic count and larger tumor size, was an independent factor for poor prognosis in patients with localized GISTs.

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“…Iwashita et al [12] reported that cellularity, type of growth, and clinical findings concerning the tumor (for example, necrosis, hemorrhage, and ulceration) were all useful in determining its malignancy. A proliferation index such as the MIB-1 index can be used for assessing prognosis [13][14][15][16][17]. Carrillo et al [13] reported that a high MIB-1 index (Ͼ22%, in the most active area) was the most powerful predictor of poor survival.…”

Section: Discussionmentioning

confidence: 99%

“…A proliferation index such as the MIB-1 index can be used for assessing prognosis [13][14][15][16][17]. Carrillo et al [13] reported that a high MIB-1 index (Ͼ22%, in the most active area) was the most powerful predictor of poor survival. The highest MIB-1 index value for the present patient was 25.5%, suggesting the malignant nature of the tumor.…”

Section: Discussionmentioning

confidence: 99%

A gastrointestinal stromal tumor in the stomach: usefulness of computed tomographic volumetry

et al. 2004

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Prognostic significance of DNA ploidy and proliferative index (MIB-1 index) in gastrointestinal stromal tumors

Cited by 110 publications

References 38 publications

Expression of COX-2, PCNA, Ki-67 and p53 in gastrointestinal stromal tumors and its relationship with histopathological parameters

Expression of COX-2, PCNA, Ki-67 and p53 in gastrointestinal stromal tumors and its relationship with histopathological parameters

Prognostic Significance of c-kit Mutation in Localized Gastrointestinal Stromal Tumors

A gastrointestinal stromal tumor in the stomach: usefulness of computed tomographic volumetry

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