Prognostic significance of clinical parameters and Epstein-Barr virus infection in non-endemic undifferentiated carcinoma of nasopharyngeal type: a Serbian report

Abstract

Undifferentiated carcinoma of nasopharyngeal type (UCNT) is very rare tumour in Serbia, like in most of the countries of Europe, with incidence less than 0.5 per 100,000 people per year. The aim of this study was to assess the presence of Epstein-Barr virus (EBV) in the UCNT of a non-endemic population in Serbia and identify the main clinical parameters that interfere with patients' survival rate. This study included 102 patients with UCNT who were diagnosed between 1996 and 2003. Biopsies were analysed for EBV-encoded RNA (EBER) by in situ hybridization of tumour tissue microarray. Of 102 patients, 76 were men and 26 were women with ages ranging between 18 and 82 years (median 52.5, mean 53.0±14.1). Survival rates were 80, 39 and 31% for one, three and five years, respectively. Ninety-three of 102 cases were EBER positive (92%). Factors with unfavourable prognostic values were age over 50 years at the time of diagnosis, advanced clinical stage, therapy other than chemoradiotherapy and EBER negative status. In regard to the clinical data, EBER expression in UCNT was shown to be a strong independent predictor of overall and progression-free survival. To our knowledge, the current report constitutes the largest European non-endemic series of UCNT samples from a single institution with correlation between survival and clinical parameters/EBER status.

Human papillomavirus and Epstein–Barr virus in nasopharyngeal carcinoma in a low‐incidence population

“…In addition, for the first time, we show that HPV‐positive NPCs seem to have OS similar to that of EBV‐positive NPC. Others have reported favorable prognostic significance of EBV‐positivity in NPC in low‐incidence population (with unknown HPV status) . Routine testing of EBV‐negative NPC for HPV/p16 may help to identify a subset of NPC with worse outcome (ie, patients with EBV‐negative and HPV‐negative NPC).…”

“…Others have reported favorable prognostic significance of EBV-positivity in NPC in low-incidence population (with unknown HPV status). 21 Routine testing of EBV-negative NPC for HPV/p16 may help to identify a subset of NPC with worse outcome (ie, patients with EBV-negative and HPV-negative NPC). Previously, HPV was detected in NPC with keratinizing 22,23 and nonkeratinizing 3 histology.…”

Carboxy‐terminal sequence variation of LMP1 gene in Epstein–Barr‐virus‐associated mononucleosis and tumors from Serbian patients

“…In Serbia, a non‐endemic region for EBV infections, reports of the virus in patients with EBV‐associated diseases have been rare to date [Terzic et al, 2010]. However, there are no data about LMP1 sequence variations and strains.…”

Pemeriksaan EBER sebagai identifikasi infeksi virus Epstein-Barr pada karsinoma nasofaring

“…Studi terbaru menyatakan bahwa jalur EBER berperan pada onkogenesis VEB. 2,3,10,11 Protein Epstein-Barr virus encoded small RNA menginduksi ekspresi sitokin yang berperan sebagai faktor pertumbuhan autokrin, antara lain interleukin 10 (IL-10) pada sel limfoma Burkitt, IL-9 pada limfoma sel T, dan insulin like growth factor 1 (IGF1) pada sel KNF yang menyebabkan pertumbuhan dan proliferasi sel tidak terkontrol. Protein EBER juga menyebabkan sel menjadi resisten terhadap apoptosis melalui mekanisme hambatan terhadap aktivitas RNA-dependent protein kinase (PKR).…”

“…Penggunaan kayu bakar, paparan panas industri, dan produk pembakaran juga meningkatkan risiko KNF 2 kali lipat. 11 Mayoritas penderita KNF adalah wiraswasta, atau bekerja seperti tukang las dan pedagang kaki lima yang mempunyai aktivitas di luar rumah lebih lama. Paparan sinar ultra violet, asap kendaraan bermotor, dan polusi udara dalam jangka waktu lama, dimungkinkan dapat meningkatkan risiko KNF.…”