Prognostic significance of ARL9 and its methylation in low-grade glioma

Tan, Yutang; Zhang, Suojun; Xiao, Qungen; Wang, Junwen; Zhao, Kai; Liu, Weihua; Huang, Kuan; Tian, Weidong; Niu, Hongquan; Lei, Ting; Shu, Kai

doi:10.1016/j.ygeno.2020.08.035

Cited by 40 publications

(38 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Almost all sites were signi cantly related to OS, which veri ed the prognostic relevance of EMILIN2. Many studies have indicated that the relationship between speci c DNA methylation sites and the expression of neighbouring genes ranges from weak to moderate, and there are few genes that are regulated by DNA methylation [1,[5][6][7]. In our study, we found that EMILIN2 was obviously and negatively regulated by its DNA methylation.…”

Section: Discussion/conclusionsupporting

confidence: 51%

See 1 more Smart Citation

EMILIN2 Correlated With Its Methylation and Immune Infiltration Could Be an Independent Prognostic Biomarker in LGG

Wang¹,

Zhang²,

Tan³

et al. 2021

Preprint

View full text Add to dashboard Cite

Low-grade gliomas (LGGs) are slow-growing brain cancer in central nervous system neoplasms. EMILIN2 is an extracellular matrix (ECM) protein which could influence the progress of some tumour which is unclear in LGG. In our study, the methylation, expression, prognosis and immune value of EMILIN2 were analysed in LGG through bioinformatics analysis. we first analysed the LGG data from TCGA and discovered that the EMILIN2 expression, negatively correlated to the EMILIN2 methylation could predict a poor prognosis and associated with different clinical parameters. Moreover, univariate and multivariate Cox regression were performed in CGGA showed that the EMILIN2 could be an independent prognostic biomarker in LGG. Finally, EMILIN2 expression showed a correlation with gene makers in some immune cells which identified the significance of EMILIN2 in immune infiltration. In conclusion, EMILIN2 could act as an independent prognostic biomarker which might be associated with the malignancy and development of gliomas and play a crucial role in glioma in immune infiltration.

show abstract

Section: Discussion/conclusionsupporting

confidence: 51%

“…Low-grade glioma (LGG) is a common primary malignant brain tumour that has great intrinsic heterogeneity in tumour biological behaviour [1]. Grade II and III gliomas, which have a median survival of more than 7 years, are collectively termed diffuse lower-grade gliomas [2][3].…”

Section: Introductionmentioning

confidence: 99%

EMILIN2 Correlated With Its Methylation and Immune Infiltration Could Be an Independent Prognostic Biomarker in LGG

Wang¹,

Zhang²,

Tan³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…We evaluated the differences between the high- and low-risk groups in the abundance of 22 immune cells and the expression levels of immune-related molecules. Besides, we applied Pearson correlation analysis to calculate the correlation between risk scores and the expression levels of immune cell markers, which were well elucidated by previous studies ( Danaher et al, 2017 ; Tan et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

A Prognostic Ferroptosis-Related lncRNAs Signature Associated With Immune Landscape and Radiotherapy Response in Glioma

Zheng

Zhou

Qiu

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Recent studies have demonstrated that long non-coding RNAs (lncRNAs) are implicated in the regulation of tumor cell ferroptosis. However, the prognostic value of ferroptosis-related lncRNAs has never been comprehensively explored in glioma. In this study, the transcriptomic data and clinical information of glioma patients were downloaded from TCGA, CGGA and Rembrandt databases. We identified 24 prognostic ferroptosis-related lncRNAs, 15 of which (SNAI3-AS1, GDNF-AS1, WDFY3-AS2, CPB2-AS1, WAC-AS1, SLC25A21-AS1, ARHGEF26-AS1, LINC00641, LINC00844, MIR155HG, MIR22HG, PVT1, SNHG18, PAXIP1-AS2, and SBF2-AS1) were used to construct a ferroptosis-related lncRNAs signature (FRLS) according to the least absolute shrinkage and selection operator (LASSO) regression. The validity of this FRLS was verified in training (TCGA) and validation (CGGA and Rembrandt) cohorts, respectively. The Kaplan-Meier curves revealed a significant distinction of overall survival (OS) between the high- and low-risk groups. The receiver operating characteristic (ROC) curves exhibited robust prognostic capacity of this FRLS. A nomogram with improved accuracy for predicting OS was established based on independent prognostic factors (FRLS, age, and WHO grade). Besides, patients in the high-risk group had higher immune, stroma, and ESTIMATE scores, lower tumor purity, higher infiltration of immunosuppressive cells, and higher expression of immune checkpoints. Patients in the low-risk group benefited significantly from radiotherapy, while no survival benefit of radiotherapy was observed for those in the high-risk group. In conclusion, we identified the prognostic ferroptosis-related lncRNAs in glioma, and constructed a prognostic signature which was associated with the immune landscape of glioma microenvironment and radiotherapy response.

show abstract

“…HIST1H2BK was identified as an indicator of poor prognosis and a promising biomarker for the treatment of low-grade glioma (LGG) ( Liu et al, 2020 ). Similarly, ARL9 had been shown its prognostic value in LGG, and probably played an important role in immune cell infiltration ( Tan et al, 2020 ). All these markers are possible used for advanced decision-making processes in the future; however, many more potential prognostic indicators still need to be explored in gliomas.…”

Section: Introductionmentioning

confidence: 96%

The relationship between PLOD1 expression level and glioma prognosis investigated using public databases

Tian

Zhou

Wang

et al. 2021

PeerJ

View full text Add to dashboard Cite

Background Glioma is the most common type of intracranial tumor with high malignancy and poor prognosis despite the use of various aggressive treatments. Targeted therapy and immunotherapy are not effective and new biomarkers need to be explored. Some Procollagen-lysine 2-oxyglutarate 5-dioxygenase (PLOD) family members have been found to be involved in the metastasis and progression of tumors. Both PLOD2 and PLOD3 had been reported to be highly expressed in gliomas, while the prognostic value of PLOD1 remains to be further illustrated, so we want to investigate the PLOD1 expression in glioma and its clinical implication. Methods We collected gene expression and corresponding clinical data of glioma from the Chinese Glioma Genome Atlas (CGGA) database, The Cancer Genome Atlas (TCGA) database and the Gene Expression Omnibus (GEO) database. First, we analyzed the expression and mutation of PLOD1 in gliomas and its relationship with clinicopathologic characteristics. Then, we conducted survival analysis, prognostic analysis and nomogram construction of the PLOD1 gene. Finally, we conducted gene ontology (GO) enrichment analysis and gene set enrichment analysis (GSEA) to explore possible mechanisms and gene co-expression analysis was also be performed. Results The results showed that the expression level of PLOD1 was higher in gliomas than normal tissues, and high expression of PLOD1 was related to poor survival which can serve as an oncogenic factor and an independent prognostic indicator for glioma patients. Both the GO and GSEA analysis showed high expression of PLOD1 were enriched in Extracellular matrix (ECM) related pathways, the co-expression analysis revealed that PLOD1 was positively related to HSPG2, COL6A2, COL4A2, FN1, COL1A1, COL4A1, CD44, COL3A1, COL1A2 and SPP1, and high expression of these genes were also correlated to poor prognosis of glioma. Conclusions The results showed that high expression of PLOD1 leads to poor prognosis, and PLOD1 is an independent prognostic factor and a novel biomarker for the treatment of glioma. Furthermore, targeting PLOD1 is most likely a potential therapeutic strategy for glioma patients.

show abstract

Prognostic significance of ARL9 and its methylation in low-grade glioma

Cited by 40 publications

References 33 publications

EMILIN2 Correlated With Its Methylation and Immune Infiltration Could Be an Independent Prognostic Biomarker in LGG

EMILIN2 Correlated With Its Methylation and Immune Infiltration Could Be an Independent Prognostic Biomarker in LGG

A Prognostic Ferroptosis-Related lncRNAs Signature Associated With Immune Landscape and Radiotherapy Response in Glioma

The relationship between PLOD1 expression level and glioma prognosis investigated using public databases

Contact Info

Product

Resources

About