“…In previous studies concerning conjunctival and uveal melanomas, the proliferation rate has been assessed by the silver staining of the nucleolar organizer regions [14,29]; nevertheless in these studies, by a not standardised and time consuming procedure, AgNORs have been counted in one-hundred cells per specimen, while no informations about the area of argyrophilic nucleolar organizer regions have been furnished [3,14,29]. More recently, two papers coming from the same south area of Italy have been performed on uveal melanomas, utilizing a different manner of AgNOR determination as well as different morphometric parameters and reaching dissonant conclusions [3,30]. In detail, the multivariate analysis didn't provide any additional information about prognosis, although patients having a high AgNOR count had a worse survival [3]; on the other hand, patients with the highest values of AgNOR mean area showed poorer clinical behaviour, even if no correlation was found between AgNORs and largest tumour dimensions [30].…”