Prognosis Value of microRNA-3677-3p in Lung Adenocarcinoma and Its Regulatory Effect on Tumor Progression

Abstract

Purpose Lung adenocarcinomas (LUAD) was the most common subtype of lung cancer, and may result in a poor prognosis. This study was designed to explore the role of miR-3677-3p in LUAD and discuss in what way it functions in LUAD. Materials and Methods We used RT-qPCR method to detect the expression levels of miR-3677-3p in 105 pairs of LUAD tissues and noncancerous tissues, as also as in LUAD cells. We used χ 2 test to analyze the correlation between miR-3677-3p level and the clinical data. The prognosis significance of miR-3677-3p was inferred with Kaplan–Meier and multivariate Cox regression assays. Biological functions of LUAD cells were accessed by cell counting kit-8, transwell migration and invasion assay. The target gene of miR-3677-3p was investigated by luciferase activity assay. Results miR-3677-3p represented an ascendant expression in LUAD tissue specimens and cells. miR-3677-3p expression was associated with the TNM stage and with solitary metastasis. Over-expression of miR-3677-3p can shorten the overall survival period of LUAD patients when compared with low expression. Knockdown of miR-3677-3p suppressed the biology function of NSCLC cells including proliferation, migration, and invasion. KLF12 was a target gene of miR-3677-3p. Conclusion miR-3677-3p represents as a potential prognostic biomarker for LUAD. miR-3677-3p can promote LUAD progression by targeting KLF12 .

Expression and Correlation Research of MicroRNA10a-5p and PIK3CA in Middle Ear Cholesteatoma

“…Yoshikawa et al 15 intraperitoneally injected synthetic miR-214 into mice with canine angiosarcoma and observed that it produced antitumor effects by inducing apoptosis and inhibiting cell proliferation. In addition, miRNA expression has been shown to affect the occurrence, development, and prognosis of various malignancies, including lung cancer, 16 prostate cancer, 17 and liver cancer. 18 Since the critical role of miRNAs in cancer has been demonstrated, gene therapy based on miRNA has attracted considerable attention.…”