Prognosis of Esophageal Squamous Cell Carcinoma: Analysis of Clinicopathological and Biological Factors

Wang, Liang Shun; Chow, Kuan Chih; Hwa, Kwan; Liu, Chia Chuan; Li, Wing Yin; Chiu, Jen‐Hwey; Huang, Min Hsiung

doi:10.1111/j.1572-0241.1999.01233.x

Cited by 89 publications

(49 citation statements)

References 44 publications

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“…This has been well established in previous studies conducted with nearly the same immunohistochemical procedures, together with a lack of correlation to prognosis, which is consistent with our findings (Sarbia et al, 1994;Hardwick et al, 1997;Shimada et al, 1999;Wang et al, 1999;Ahn et al, 2002;Rosa et al, 2003). P53 mutations are Diffuse EGFR staining and reduced overall survival L Gibault et al peculiarly frequent in high-SCCO incidence areas like Brittany (Audrezet et al, 1993), where we performed our study; some geographic specificity might also be implicated.…”

Section: P53 Protein In Sccosupporting

confidence: 92%

Diffuse EGFR staining is associated with reduced overall survival in locally advanced oesophageal squamous cell cancer

et al. 2005

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Squamous cell carcinoma of the oesophagus (SCCO) is still a pathology of bad prognosis. Specific therapies are now developed against epidermal growth factor receptor (EGFR), human epidermal growth factor receptor 2, c-kit receptor (CD117), vascular endothelial growth factor (VEGF) and p53 protein. This study was aimed at assessing their expression in a large series of SCCO, as well as their potential therapeutic interest in this pathology. Immunohistochemical expression of these factors was assessed retrospectively in 107 cases of SCCO with primary surgery, as well as their relationships to recurrence, metastasis and overall survival on a long-term follow-up. Human epidermal growth factor receptor 2 and CD117 were expressed in less than 3% of the cases. Epidermal growth factor receptor and p53 were overexpressed in 68.2 and 66.4% of the cases, and VEGF in 38.3%. Epidermal growth factor receptor overexpression was significantly related to vascular invasion (P ¼ 0.023). Its diffuse positivity was significantly related in multivariate analysis to higher local recurrence (P ¼ 0.006) and lower overall survival (P ¼ 0.003), in a subgroup of patients of poor outcome who had received postoperative adjuvant treatment. These results highlight the great potential prognostic and therapeutic interest of evaluating EGFR diffuse positivity in locally advanced SCCO.

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Section: P53 Protein In Sccosupporting

confidence: 92%

Diffuse EGFR staining is associated with reduced overall survival in locally advanced oesophageal squamous cell cancer

et al. 2005

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“…Therefore, our result was presumably influenced by the factors of cell differentiation, tumour invasion, and lymph node metastasis. Some reports have demonstrated that the degree of cell differentiation is a useful prognostic factor in ESCC patients (Torres et al, 1999;Wang et al, 1999). Invasion and metastasis, the main causes of death in most cancer patients, remain the most important but least understood aspects of cancer.…”

Section: Fak-overexpression (−) Fak-overexpression (+)mentioning

confidence: 99%

FAK overexpression is correlated with tumour invasiveness and lymph node metastasis in oesophageal squamous cell carcinoma

et al. 2003

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Focal adhesion kinase (p125 FAK ; 'FAK') is a tyrosine kinase that is localised to cellular focal adhesions and is associated with a number of other proteins, such as integrin adhesion receptors. We performed an immunohistochemical analysis of FAK protein expression to determine the relationship between FAK overexpression and clinicopathological factors in oesophageal squamous cell carcinoma (ESCC). We examined tissue specimens that had been removed from 91 patients with thoracic oesophageal cancer who had undergone surgery between 1983 and 2001. Immunohistochemical staining was performed by the standard streptavidin -biotin method. Seven human ESCC cell lines -TE-1, TE-2, TE-8, TE-13, TE-15, TT, and TTn -and one immortalized human keratinocyte cell line -HaCaT -were used in Western blot analysis. Immunostaining of FAK was seen in the cytoplasm of cancer cells, particularly in cells located in the invasive fronts of cancer nests. FAK overexpression was detected in 54 of the 91 patients (59.3%). Significant correlations were observed between FAK overexpression and cell differentiation (P ¼ 0.0057), depth of tumour invasion (P ¼ 0.0023), presence of regional lymph node metastasis (P ¼ 0.0097), number of lymph node metastases (P ¼ 0.0026), and disease stage (P ¼ 0.012). The survival rates of patients with FAK-overexpressing cancer were significantly lower than those of patients without FAK-overexpression cancer (P ¼ 0.006). The 5-year survival rate of patients without FAK overexpression was 69%, whereas that of patients with FAK overexpression was 38%. On Western blot analysis, FAK was expressed at a high level in TE-1, TE-8, TE-15, and TT cells, at a moderate level in TE-2 and TTn cells, and at a low level in TE13 and HaCaT cells. FAK phosphorylation at tyrosine 397 was demonstrated in proportion to the intensity of FAK in all cell lines except TE15 and HaCaT. In conclusion, FAK overexpression of ESCC was related to cell differentiation, tumour invasiveness, and lymph node metastasis. Consequently, patients with ESCC who had FAK overexpression had a poor prognosis.

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“…Expression of nm23-H1 in the pathologic sections was detected by an immunoperoxidase method as previously described (Wang et al, 1999a(Wang et al, , 2002. Paraffin blocks were sectioned at the thickness of 4 mm.…”

Section: Immunohistochemical Stainingmentioning

confidence: 99%

Prognostic significance of nm23-H1 expression in oral squamous cell carcinoma

et al. 2004

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Recent studies indicated nm23-H1 played a role in cancer progression. Therefore, we investigated clinical significance of nm23-H1 expression in oral squamous cell carcinoma (OSCC). In total, 86 OSCC specimens were immunohistochemically stained with nm23-H1-specific monoclonal antibodies. Immunohistochemical staining of nm23-H1 was confirmed by immunoblotting. The relations between nm23-H1 expression and clinicopathologic variables were evaluated by w 2 analysis. As increased size of primary tumour could escalate metastatic potential and the data of patients at the late T stage might confound statistical analyses, we thus paid special attention to 54 patients at the early T stage of OSCC. Statistical difference of survival was compared by a log-rank test. Immunohistochemically, nm23-H1 expression was detected in 48.8% (42 out of 86) of tumorous specimens. It positively correlated with larger primary tumour size (P ¼ 0.03) and inversely with cigarette-smoking habit (P ¼ 0.042). In patients at the early T stage, decreased nm23 expression was associated with increased incidence of lymph node metastasis (P ¼ 0.004) and indicated poor survival (P ¼ 0.014). Tumour nm23-H1 expression is a prognostic factor for predicting better survival in OSCC patients at the early T stage, which may reflect antimetastatic potential of nm23. Therefore, modulation of nm23-H1 expression in cancer cells can provide a novel possibility of improving therapeutic strategy at this stage. In addition, our results further indicated cigarette smoking could aggravate the extent of nm23-H1 expression and possibly disease progression of OSCC patients.

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Prognosis of Esophageal Squamous Cell Carcinoma: Analysis of Clinicopathological and Biological Factors

Cited by 89 publications

References 44 publications

Diffuse EGFR staining is associated with reduced overall survival in locally advanced oesophageal squamous cell cancer

Diffuse EGFR staining is associated with reduced overall survival in locally advanced oesophageal squamous cell cancer

FAK overexpression is correlated with tumour invasiveness and lymph node metastasis in oesophageal squamous cell carcinoma

Prognostic significance of nm23-H1 expression in oral squamous cell carcinoma

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