Progesterone receptor gene expression in preimplantation pig embryos

Ying, Chingwen; Yang, YC; Hong, Wei‐Fong; Cheng, Weijia; Hsu, Wei‐Lun

doi:10.1530/eje.0.1430697

Cited by 21 publications

(16 citation statements)

References 33 publications

(29 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Expression of PGRs in embryos has been demonstrated in several species including mouse (Hou & Gorski 1993), pig (Ying et al 2000) and horse (Rambags et al 2008). To our knowledge, the present study shows for the first time PGR mRNA expression in bovine preimplantation embryos.…”

Section: Discussionsupporting

confidence: 58%

Progesterone and conceptus elongation in cattle: a direct effect on the embryo or an indirect effect via the endometrium?

Clemente¹,

Fuente²,

Fair³

et al. 2009

Reproduction

292

225

View full text Add to dashboard Cite

The steroid hormone progesterone (P 4 ) plays a key role in the reproductive events associated with pregnancy establishment and maintenance. High concentrations of circulating P 4 in the immediate post-conception period have been associated with an advancement of conceptus elongation, an associated increase in interferon-t production and higher pregnancy rates in cattle. Using in vitro and in vivo models and w8500 bovine oocytes across six experiments, the aim of this study was to establish the route through which P 4 affects bovine embryo development in vitro and in vivo. mRNA for P 4 receptors was present at all stages of embryo development raising the possibility of a direct effect of P 4 on the embryo. Exposure to P 4 in vitro in the absence or presence of oviduct epithelial cells did not affect the proportion of embryos developing to the blastocyst stage, blastocyst cell number or the relative abundance of selected transcripts in the blastocyst. Furthermore, exposure to P 4 in vitro did not affect post-hatching elongation of the embryo following transfer to synchronized recipients and recovery on Day 14. By contrast, transfer of in vitro derived blastocysts to a uterine environment previously primed by elevated P 4 resulted in a fourfold increase in conceptus length on Day 14. These data provide clear evidence to support the hypothesis that P 4 -induced changes in the uterine environment are responsible for the advancement in conceptus elongation reported previously in cattle and that, interestingly, the embryo does not need to be present during the period of high P 4 in order to exhibit advanced elongation.

show abstract

Section: Discussionsupporting

confidence: 58%

Progesterone and conceptus elongation in cattle: a direct effect on the embryo or an indirect effect via the endometrium?

Clemente¹,

Fuente²,

Fair³

et al. 2009

Reproduction

292

225

View full text Add to dashboard Cite

show abstract

“…In the developing mouse embryo, ERα, ERβ, and PR are expressed at the blastocyst stage and thereafter [16][17][18][19][20][21][22][23]. The expression of these receptors in mES cells may not directly reflect the same timing as in development, but their expression in mES cells allows us to use it as a model system of in vitro differentiation.…”

Section: Discussionmentioning

confidence: 99%

“…Other members of the nuclear receptor family, estrogen receptor alpha (ERα), estrogen receptor beta (ERβ), and progesterone receptor (PR) are present in the early developing embryo of mice and other species [16][17][18][19][20][21][22][23]. In particular, ERα is present in the oocyte, shows variable expression through the morula stage, followed by increased expression in the inner cell mass of the blastocyst.…”

Section: Introductionmentioning

confidence: 99%

Differentiation of murine embryonic stem cells induces progesterone receptor gene expression

Sauter

McDermid

Weinberg

et al. 2005

Experimental Cell Research

View full text Add to dashboard Cite

The role of steroid hormone receptors in very early embryonic development remains unknown. Clearly, expression during organogenesis is important for tissue-specific development. However, progesterone receptor (PR) and estrogen receptors (ERα, ERβ), are expressed during early development through the blastocyst stage in mice and other species, and yet are not essential for embryonic viability. We have utilized the mouse embryonic stem (mES) cell model to investigate the regulated expression of these receptors during differentiation. Surprisingly, one of the earliest changes in gene expression in response to a differentiation signal observed is PR gene induction. It parallels the time course of expression for the patterning genes Hoxb1 and Hoxa5. Unexpectedly, PR gene expression is not regulated in an estrogen dependent manner by endogenous ERs or by transiently overexpressed ERα. Our results suggest a potentially novel mechanism of PR gene regulation within mES cells compared to adult tissues and the possibility of unique targets of PR action during early mES cell differentiation

show abstract

“…The P4R protein mediates the physiological effects of progesterone, the main hormone produced by the corpus luteum. Progesterone is responsible for the implantation and maintenance of pregnancy in mammals, playing a key role in a series of coordinated steps comprising the implantation period of the embryo into the uterus (Ying et al, 2001). Peralta et al (2005) analyzed the expression of progesterone receptors in the oviduct of sows during the follicular and luteal phase of the estrus cycle.…”

Section: Resultsmentioning

confidence: 99%

Differential expression of genes in follicular cells of swines

et al. 2010

View full text Add to dashboard Cite

-The main purpose of the present study was to identify for candidate genes related to ovulation in swines.To do so, it was investigated in ovarian follicular cells through quantitative real-time PCR the differential expression of the following genes: steroidogenic acute regulator (STAR), GATA-binding protein 4 (GATA), prostaglandin F2α (PGF2α), progesterone receptor (P4R), follicle-stimulating hormone receptor (FSHR), and cytochrome P450 aromatase (CYP19).These genes encode hormone receptors (FSHR and P4R), hormone (PGF2α), steroidogenic proteins (STAR and CYP19) and transcription factor (GATA). Folicular cells were collected from sows with high and low number of piglets/litters during the follicular phase of the estrus cycle. There was difference in transcript abundance among low and high prolific sows for the STAR, GATA, PGF2α, P4R and CYP19 genes. For the FSHR gene, the fold change was not considered to be significantly different. Because in the present study only the transcript level of the above mentioned genes was analyzed, no inference can be made regarded to protein translation or activity. Therefore, gene sequence trials and other functional studies will be necessary to complement the present results, allowing a better understanding on biological complexity of these genes and their use as markers for prolificity in swines.Key Words: candidate gene, litter size, ovulation rate, pig production, quantitative real-time PCR Expressão diferencial de genes em células foliculares de suínosRESUMO -O objetivo neste trabalho foi identificar genes candidatos relacionados à ovulação em suínos. Para tanto, investigou-se a expressão diferencial dos genes STAR (steroidogenic acute regulator), GATA (GATA-binding protein 4), PGF2α (prostaglandin F2α), P4R (progesterone receptor), FSHR (follicle-stimulating hormone receptor) e CYP19 (cytochrome P450 aromatase) em células foliculares ovarianas por meio de reação em cadeia da polimerase em tempo real (qRT-PCR) quantitativo em tempo real. Esses genes codificam para receptores hormonais (FSHR e P4R) hormônio (PGF2α), proteínas esteroidogênicas (STAR e CYP19) e fator de transcrição (GATA). As células foliculares foram coletadas durante a fase folicular do ciclo estral de porcas com alto e baixo número de leitões/leitegada. Houve diferença na abundância de transcritos entre porcas com alta e baixa prolificidade para os genes STAR, GATA, PGF2α, P4R and CYP19. Para o gene do FSHR, a alteração na abundância dos transcritos não foi significativamente diferente. Considerando que foi analisado somente o nível de transcrição desses genes mencionados, não se pode fazer inferências com relação à tradução ou atividade proteica. Portanto, ensaios de sequenciamento gênico e outras análises funcionais serão necessários para complementar esses achados e possibilitar melhor entendimento da complexidade biológica desses genes e seu uso como marcadores para prolificidade em suínos.Palavras-chave: genes candidatos, ovulação, produção de suínos, PCR quantitativo em tempo real, tamanho...

show abstract

Progesterone receptor gene expression in preimplantation pig embryos

Cited by 21 publications

References 33 publications

Progesterone and conceptus elongation in cattle: a direct effect on the embryo or an indirect effect via the endometrium?

Progesterone and conceptus elongation in cattle: a direct effect on the embryo or an indirect effect via the endometrium?

Differentiation of murine embryonic stem cells induces progesterone receptor gene expression

Differential expression of genes in follicular cells of swines

Contact Info

Product

Resources

About