Differentiation of murine embryonic stem cells induces progesterone receptor gene expression

Sauter, Carley N.; McDermid, Rebecca L.; Weinberg, Amy L.; Greco, Tamara L.; Xu, Xiaojie; Murdoch, Fern E.; Fritsch, Michael K.

doi:10.1016/j.yexcr.2005.09.005

Cited by 29 publications

(32 citation statements)

References 75 publications

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“…The last set of genes, termed "OFF," were not detectable during the early stages of differentiation examined in these studies. Afp (endoderm) and ␤H1 globin (later mesoderm) did not increase significantly until after 5 days of LIF withdrawal (6). Prl and Ins are tissue-specific genes that were not expressed after LIF withdrawal in our model system even after longer induction times.…”

Section: Global Histone Acetylation Increased During Early Differentimentioning

confidence: 65%

“…PR is a complex gene, which we have recently shown is turned on in mES cells 12-24 h after LIF withdrawal ( Fig. 1) (6). Two isoforms of PR, PRA and PRB, are encoded by a single gene with two separate promoters (18).…”

Section: Localized Histone Acetylation Patterns In Gene Regulatory Rementioning

confidence: 88%

“…Preparation of Total RNA-RNA samples were isolated from cultured cells (usually one 10-cm plate/sample) using Trizol reagent (Invitrogen) as previously described (6).…”

Section: Methodsmentioning

confidence: 99%

“…Conventional Reverse Transcription-PCR-The synthesis of cDNA and performance of conventional PCR was as previously reported (6). Additional primers used and detection are described in the supplemental data.…”

Section: Methodsmentioning

confidence: 99%

“…A common method of inducing differentiation is withdrawal of LIF from the culture media, resulting in multi-lineage differentiation, indicated by the induced expression of lineage marker genes (6). During early mES cell differentiation, the rate of transcription of large numbers of genes is substantially altered in a time-dependent fashion.…”

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confidence: 99%

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The Role of Histone Acetylation in Regulating Early Gene Expression Patterns during Early Embryonic Stem Cell Differentiation

McCool

Singer

et al. 2007

Journal of Biological Chemistry

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We have examined the role of histone acetylation in the very earliest steps of differentiation of mouse embryonic stem cells in response to withdrawal of leukemia inhibitory factor (LIF) as a differentiation signal. The cells undergo dramatic changes in morphology and an ordered program of gene expression changes representing differentiation to all three germ layers over the first 3-5 days of LIF withdrawal. We observed a global increase in acetylation on histone H4 and to a lesser extent on histone H3 over this time period. Treatment of the cells with trichostatin A (TSA), a histone deacetylase inhibitor, induced changes in morphology, gene expression, and histone acetylation that mimicked differentiation induced by withdrawal of LIF. We examined localized histone acetylation in the regulatory regions of genes that were transcriptionally either active in undifferentiated cells, induced during differentiation, or inactive under all treatments. There was striking concordance in the histone acetylation patterns of specific genes induced by both TSA and LIF withdrawal. Increased histone acetylation in local regions correlated best with induction of gene expression. Finally, TSA treatment did not support the maintenance or progression of differentiation. Upon removal of TSA, the cells reverted to the undifferentiated phenotype. We concluded that increased histone acetylation at specific genes played a role in their expression, but additional events are required for maintenance of differentiated gene expression and loss of the pluripotent state.

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Section: Global Histone Acetylation Increased During Early Differentimentioning

confidence: 65%

Section: Localized Histone Acetylation Patterns In Gene Regulatory Rementioning

confidence: 88%

“…Preparation of Total RNA-RNA samples were isolated from cultured cells (usually one 10-cm plate/sample) using Trizol reagent (Invitrogen) as previously described (6).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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The Role of Histone Acetylation in Regulating Early Gene Expression Patterns during Early Embryonic Stem Cell Differentiation

McCool

Singer

et al. 2007

Journal of Biological Chemistry

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Mifepristone-Inducible Caspase-1 Expression in Mouse Embryonic Stem Cells Eliminates Tumor Formation but Spares Differentiated Cells In Vitro and In Vivo

et al. 2012

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Embryonic stem cell (ESC)-based therapy is a promising treatment for neurodegenerative diseases. But there is always a risk of tumor formation that is due to contamination of undifferentiated ESCs. To reduce the risk and improve ESC-based therapy, we have established a novel strategy by which we can selectively eliminate tumor cells derived from undifferentiated ESCs but spare differentiated cells. In this study, we generated a caspase-1-ESC line transfected with a mifepristone-regulated caspase-1 expression system. Mifepristone induced caspase-1 overexpression both in differentiated and undifferentiated caspase-1-ESCs. All the undifferentiated caspase-1-ESCs were induced to death after mifepristone treatment. Tumors derived from undifferentiated caspase-1-ESCs were eliminated following 3 weeks of mifepristone treatment in vivo. However, differentiated caspase-1-ESCs survived well under the condition of mifepristone-induced caspase-1 overexpression. To examine in vivo the impact of mifepristone-induced caspase-1 activation on grafted cells, we transplanted wild-type ESCs or caspase-1-ESCs into nude mice brains. After 8 weeks of mifepristone treatment, we could not detect any tumor cells in the caspase-1-ESC grafts in the brains of mice. However, we found that donor dopamine neurons survived in the recipient brains. These data demonstrate that mifepristone-induced caspase-1 overexpression in ESCs can eliminate the potential tumor formation meanwhile spares the differentiated cells in the host brains. These results suggest that this novel ESCbased therapy can be used in Parkinson's disease and other related disorders without the risk of tumor formation. STEM CELLS 2012;30:169-179 Disclosure of potential conflicts of interest is found at the end of this article.

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Concise Review: Androgen Receptor Differential Roles in Stem/Progenitor Cells Including Prostate, Embryonic, Stromal, and Hematopoietic Lineages

et al. 2014

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Stem/progenitor (S/P) cells are special types of cells that have the ability to generate tissues throughout their entire lifetime and play key roles in the developmental process. Androgen and the androgen receptor (AR) signals are the critical determinants in male gender development, suggesting that androgen and AR signals might modulate the behavior of S/P cells. In this review, we summarize the AR effects on the behavior of S/P cells, including self-renewal, proliferation, apoptosis, and differentiation in normal S/P cells, as well as proliferation, invasion, and self-renewal in prostate cancer S/P cells. AR plays a protective role in the oxidative stress-induced apoptosis in embryonic stem cells. AR inhibits the self-renewal of embryonic stem cells, bone marrow stromal cells, and prostate S/P cells, but promotes their differentiation except for adipogenesis. However, AR promotes the proliferation of hematopoietic S/P cells and stimulates hematopoietic lineage differentiation. In prostate cancer S/P cells, AR suppresses their self-renewal, metastasis, and invasion. Together, AR differentially influences the characteristics of normal S/P cells and prostate cancer S/P cells, and targeting AR might improve S/P cell transplantation therapy, especially in embryonic stem cells and bone marrow stromal cells.

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Differentiation of murine embryonic stem cells induces progesterone receptor gene expression

Cited by 29 publications

References 75 publications

The Role of Histone Acetylation in Regulating Early Gene Expression Patterns during Early Embryonic Stem Cell Differentiation

The Role of Histone Acetylation in Regulating Early Gene Expression Patterns during Early Embryonic Stem Cell Differentiation

Mifepristone-Inducible Caspase-1 Expression in Mouse Embryonic Stem Cells Eliminates Tumor Formation but Spares Differentiated Cells In Vitro and In Vivo

Concise Review: Androgen Receptor Differential Roles in Stem/Progenitor Cells Including Prostate, Embryonic, Stromal, and Hematopoietic Lineages

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