Progesterone lipid nanoparticles: Scaling up and in vivo human study

Esposito, Elisabetta; Sguizzato, Maddalena; Drechsler, Markus; Mariani, Paolo; Carducci, Federica; Nastruzzi, Claudio; Cortesi, Rita

doi:10.1016/j.ejpb.2017.07.015

Cited by 30 publications

(16 citation statements)

References 40 publications

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“…For example, this method was used to prepare stavudine-loaded SLNs at batch sizes of up to 60 kg [ 48 ] and coenzyme Q10 loaded-NLCs at a throughput of 25 kg/h [ 49 ]. Initially, coarse lipid particles are prepared and then forced through a homogenizer with a narrow gap of few microns at high pressure [ 29 ]. The pressures (usually 100–2000 bar) accelerate the liquid mixtures to a high velocity of >1000 km/h, which generates high shear stresses and cavitational forces that reduce droplet sizes to nano dimensions [ 1 ].…”

Section: Methods For Slns and Nlcs Preparationmentioning

confidence: 99%

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Preparation of Solid Lipid Nanoparticles and Nanostructured Lipid Carriers for Drug Delivery and the Effects of Preparation Parameters of Solvent Injection Method

2020

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Solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs) have emerged as potential drug delivery systems for various applications that are produced from physiological, biodegradable, and biocompatible lipids. The methods used to produce SLNs and NLCs have been well investigated and reviewed, but solvent injection method provides an alternative means of preparing these drug carriers. The advantages of solvent injection method include a fast production process, easiness of handling, and applicability in many laboratories without requirement of complicated instruments. The effects of formulations and process parameters of this method on the characteristics of the produced SLNs and NLCs have been investigated in several studies. This review describes the methods currently used to prepare SLNs and NLCs with focus on solvent injection method. We summarize recent development in SLNs and NLCs production using this technique. In addition, the effects of solvent injection process parameters on SLNs and NLCs characteristics are discussed.

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Section: Methods For Slns and Nlcs Preparationmentioning

confidence: 99%

“…SLNs and NLCs have been produced using numerous methods [ 28 , 29 ]. Some methods, such as high-pressure homogenization, emulsion/solvent evaporation, phase inversion, and microemulsion, have been extensively investigated and reviewed [ 2 , 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Preparation of Solid Lipid Nanoparticles and Nanostructured Lipid Carriers for Drug Delivery and the Effects of Preparation Parameters of Solvent Injection Method

2020

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“…Among lipid nanoparticles, solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs) are colloidal carriers exploiting many effects on skin, and therefore are suitable for skin applications. Figure 5 shows exemplary cryo-TEM images of tristearin SLNs (c) and NLCs (d) [ 99 , 100 , 101 ]. It has been demonstrated that the solid core of these nanoparticles is able to better sustain and control the release of drug as compared to emulsions or other vesicles, such as liposomes [ 96 ].…”

Section: Lipid-based Nanosystemsmentioning

confidence: 99%

Lipid-Based Nanosystems as a Tool to Overcome Skin Barrier

Sguizzato

Esposito

Cortesi

2021

IJMS

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Skin may be affected by many disorders that can be treated by topical applications of drugs on the action site. With the advent of nanotechnologies, new efficient delivery systems have been developed. Particularly, lipid-based nanosystems such as liposomes, ethosomes, transferosomes, solid lipid nanoparticles, nanostructured lipid carriers, cubosomes, and monoolein aqueous dispersions have been proposed for cutaneous application, reaching in some cases the market or clinical trials. This review aims to provide an overview of the different lipid-based nanosystems, focusing on their use for topical application. Particularly, biocompatible nanosystems able to dissolve lipophilic compounds and to control the release of carried drug, possibly reducing side effects, are described. Notably, the rationale to topically administer antioxidant molecules by lipid nanocarriers is described. Indeed, the structural similarity between the nanosystem lipid matrix and the skin lipids allows the achievement of a transdermal effect. Surely, more research is required to better understand the mechanism of interaction between lipid-based nanosystems and skin. However, this attempt to summarize and highlight the possibilities offered by lipid-based nanosystems could help the scientific community to take advantage of the benefits derived from this kind of nanosystem.

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“…Finally, the amount of the drug in each sample solution was evaluated through HPLC analysis and according to the calibration curve (Esposito et al, 2017).…”

Section: In Vitro Drug Releasementioning

confidence: 99%

Effects of progesterone nanoparticles on the sperm capacitation and acrosome reaction in asthenozoospermia men

et al. 2021

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Low motility is one of the causes of male infertility. In this study, the effects of progesterone solid lipid nanoparticles (SLNs) on sperm capacitation, acrosome reaction, oxidative stress and expression of SPACA1 and MAPK way genes were investigated. Progesterone SLNs were synthesized using the solvent emulsification evaporation method. Twenty asthenozoospermia samples were selected, and sperm and acrosome membrane integrity, acrosome reaction, sperm motility, viability, total antioxidant capacity (TAC), total oxidative status tests and PKA, PTK, P38MAPK and SPACA1 gene expressions were assessed. The synthesized nanoparticles were prepared with the size (187.6 nm), PDI (0.184), EE (85.82%), LP (3.43%) and ZP (−23.5mV). Progesterone SLNs increased sperm and acrosome membrane integrity and TAC (p < .05). Also, the expression of P38MAPK, PKA, PTK, and SPACA1 genes in this group showed a significant increase (p < .001). Progesterone SLNs increased acrosome reaction, sperm capacitation and TAC. Also, it increased the expression of PTK PKA, SPACA1 and P38MAPK genes.

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Progesterone lipid nanoparticles: Scaling up and in vivo human study

Cited by 30 publications

References 40 publications

Preparation of Solid Lipid Nanoparticles and Nanostructured Lipid Carriers for Drug Delivery and the Effects of Preparation Parameters of Solvent Injection Method

Preparation of Solid Lipid Nanoparticles and Nanostructured Lipid Carriers for Drug Delivery and the Effects of Preparation Parameters of Solvent Injection Method

Lipid-Based Nanosystems as a Tool to Overcome Skin Barrier

Effects of progesterone nanoparticles on the sperm capacitation and acrosome reaction in asthenozoospermia men

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