Profiling the proteome complement of the secretion from hypopharyngeal gland of Africanized nurse-honeybees ( L.)

Santos, Keity Souza; Santos, Lucilene Delazari dos; Mendes, Maria Anita; Souza, Bibiana Monson de; Malaspina, Osmar; Palma, Mário Sérgio

doi:10.1016/j.ibmb.2004.10.003

Cited by 122 publications

(121 citation statements)

References 17 publications

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“…Also Li, Feng, Zhang, and Pan (2008), who based their research on analysis of the proteome from the HPGs of honey bees, showed the presence of six different forms of MRJP-1 with molecular weights from 49 to 60 kDa. The results of these studies are in contrast to the findings of Hanes and Šimuth (1992), and correspond with the results of Santos et al (2005). Our work is the first study presenting a profile of proteolytic activity along with protein profile.…”

Section: Discussionsupporting

confidence: 68%

The influence of sublethal doses of imidacloprid on protein content and proteolytic activity in honey bees (Apis mellifera L.)

Wilde

Frączek

Siuda

et al. 2016

Journal of Apicultural Research

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The effect of sublethal doses of imidacloprid on protein content and activity of proteases on honey bees was analyzed. The study was conducted in three experimental groups: colonies from groups BE-5 and BE-200 were contaminated with 5 and 200 ppb of imidacloprid, respectively, via their food supply (syrup and pollen), while group BE was used as control (untreated). Bee samples were collected 3 and 10 weeks after feeding started. Protein concentration in bee tissue extracts was analyzed with reference: (a) to the dose of imidacloprid; and (b) duration of exposure to the chemical. The average quantity of protein content was significantly higher at the 3-week interval than in the 10-week interval and the bees from control colonies (BE) had significantly higher protein contents than contaminated bees (BE-5 and BE-200), even 3 weeks after feeding with imidacloprid started. Similarly, the activity of proteolytic enzymes (proteases) was found to be dependent on the dose of imidacloprid used, compared to bees from control colonies showing significantly higher activity.Influencia de las dosis subletales de Imidacloprid en el contenido de proteína y la actividad proteolítica en las abejas melíferas (Apis mellifera L.)Se ha analizado el efecto de las dosis subletales de imidacloprid en el contenido de proteínas y la actividad de las proteasas en las abejas melíferas. El estudio se realizó en tres grupos experimentales: las colonias de los grupos de BE-5 y BE-200 se contaminaron con 5 y 200 ppb de imidacloprid, respectivamente, a través del alimento suministrado (jarabe y polen), mientras que el grupo SER se utilizó como control (sin tratar). Se recogieron muestras de abejas en las semanas 3 y 10 después del comienzo de la alimentació n. La concentració n de proteína en extractos de tejido de abejas se analizó en relació n con: a) la dosis de imidacloprid; y b) la duració n de la exposició n a la sustancia química. La cantidad promedio del contenido de proteína fue significativamente mayor en el intervalo de 3 semanas que en el intervalo de 10 semanas y las abejas de las colonias control (BE) tuvieron un contenido de proteína significativamente más alto que las abejas contaminadas (BE-5 y BE-200), incluso 3 semanas después de que comenzara la alimentació n con imidacloprid. Del mismo modo se encontró que la actividad de las enzimas proteolíticas (proteasas) depende de la dosis de imidacloprid usada, en comparació n con las abejas de colonias de control que muestran una actividad significativamente más alta.

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Section: Discussionsupporting

confidence: 68%

The influence of sublethal doses of imidacloprid on protein content and proteolytic activity in honey bees (Apis mellifera L.)

Wilde

Frączek

Siuda

et al. 2016

Journal of Apicultural Research

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“…In silkworms, the knockdown of several GMC oxidoreductases, for example, reduced survival rates after treatment with Bacillus bombyseptieus or Escherichia coli [25]. This effect can be explained by the by-products arising during the oxidation reaction: GMC oxidoreductases produce H 2 O 2 [49], and that H 2 O 2 acts as a messenger or toxin in the immune response to microbial infections has been well described [50,51]. Interestingly, the secretions of chrysomeline larvae also have an antimicrobial effect.…”

Section: Discussionmentioning

confidence: 99%

Independently recruited oxidases from the glucose-methanol-choline oxidoreductase family enabled chemical defences in leaf beetle larvae (subtribe Chrysomelina) to evolve

et al. 2014

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Larvae of the leaf beetle subtribe Chrysomelina sensu stricto repel their enemies by displaying glandular secretions that contain defensive compounds. These repellents can be produced either de novo (iridoids) or by using plant-derived precursors (e.g. salicylaldehyde). The autonomous production of iridoids, as in Phaedon cochleariae, is the ancestral chrysomeline chemical defence and predates the evolution of salicylaldehyde-based defence. Both biosynthesis strategies include an oxidative step of an alcohol intermediate. In salicylaldehyde-producing species, this step is catalysed by salicyl alcohol oxidases (SAOs) of the glucose-methanol-choline (GMC) oxidoreductase superfamily, but the enzyme oxidizing the iridoid precursor is unknown. Here, we show by in vitro as well as in vivo experiments that P. cochleariae also uses an oxidase from the GMC superfamily for defensive purposes. However, our phylogenetic analysis of chrysomeline GMC oxidoreductases revealed that the oxidase of the iridoid pathway originated from a GMC clade different from that of the SAOs. Thus, the evolution of a host-independent chemical defence followed by a shift to a host-dependent chemical defence in chrysomeline beetles coincided with the utilization of genes from different GMC subfamilies. These findings illustrate the importance of the GMC multi-gene family for adaptive processes in plant-insect interactions.

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“…These and other protein‐coding genes are very highly expressed in nurse hypopharyngeal and mandibular glands (Santos et al. 2005; Jasper et al. 2014), and different proportions of these glandular secretions are combined with sugars and proteins and fed to larvae, depending on the age and caste trajectory of the larva (Haydak 1970; Brouwers et al.…”

Section: Introductionmentioning

confidence: 99%

The transcriptomic and evolutionary signature of social interactions regulating honey bee caste development

Vojvodic

Johnson

Harpur

et al. 2015

Ecology and Evolution

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The caste fate of developing female honey bee larvae is strictly socially regulated by adult nurse workers. As a result of this social regulation, nurse‐expressed genes as well as larval‐expressed genes may affect caste expression and evolution. We used a novel transcriptomic approach to identify genes with putative direct and indirect effects on honey bee caste development, and we subsequently studied the relative rates of molecular evolution at these caste‐associated genes. We experimentally induced the production of new queens by removing the current colony queen, and we used RNA sequencing to study the gene expression profiles of both developing larvae and their caregiving nurses before and after queen removal. By comparing the gene expression profiles of queen‐destined versus worker‐destined larvae as well as nurses observed feeding these two types of larvae, we identified larval and nurse genes associated with caste development. Of 950 differentially expressed genes associated with caste, 82% were expressed in larvae with putative direct effects on larval caste, and 18% were expressed in nurses with putative indirect effects on caste. Estimated selection coefficients suggest that both nurse and larval genes putatively associated with caste are rapidly evolving, especially those genes associated with worker development. Altogether, our results suggest that indirect effect genes play important roles in both the expression and evolution of socially influenced traits such as caste.

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Profiling the proteome complement of the secretion from hypopharyngeal gland of Africanized nurse-honeybees ( L.)

Cited by 122 publications

References 17 publications

The influence of sublethal doses of imidacloprid on protein content and proteolytic activity in honey bees (Apis mellifera L.)

The influence of sublethal doses of imidacloprid on protein content and proteolytic activity in honey bees (Apis mellifera L.)

Independently recruited oxidases from the glucose-methanol-choline oxidoreductase family enabled chemical defences in leaf beetle larvae (subtribe Chrysomelina) to evolve

The transcriptomic and evolutionary signature of social interactions regulating honey bee caste development

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