Profiling of polar metabolites in fruits of Opuntia stricta var. dillenii by ion-pair high-performance countercurrent chromatography and off-line electrospray mass-spectrometry injection

Characterization, Stability, and Bioaccessibility of Betalain and Phenolic Compounds from Opuntia stricta var. Dillenii Fruits and Products of Their Industrialization

“…These differences in the betalain profile could be related to the different agronomical conditions of the Opuntia cactus growing and the intrinsic differences among cactus of different origin. Other minor betalains such as 17-decarboxy-betanin, isobetanin, betanidin, and 17-descarboxy-Neobetanin were identified in the Opuntia dillenii samples in the present study, these being betalain compounds previously reported by Thi Tran et al [5] in the metabolome investigation of Opuntia stricta var. Dillenii fruits, recognizing them as novel fruit metabolites of betalains.…”

“…According to the statistical analysis, no significant differences were found between the peel and pulp color. Additionally, many authors reported a large number of seeds in this cactus fruit [ 1 , 5 ]; in the present study, the percent of seeds was 32.80 ± 0.46 of the weight of whole fruit. Opuntia stricta var.…”

“…However, prickly pear fruits contain a high content of important nutrients (organic acids, lipids, minerals, vitamins, etc.) and bioactive compounds, such as polysaccharides, betalains (betacyanins and betaxanthins), and phenolic compounds such as phenolic acids (piscidic acid) and flavonoids (isorhamnetin glycosides), with proven biological activities [ 5 , 6 , 7 ]. For the nutritional, health, and agronomical potential that they have, some studies have remarked upon the importance of exploiting them as food products [ 8 ].…”

“…In addition, other phenolic compounds also have chemiprotective effects due to their anti-inflammatory effect [ 6 ]. Some studies have been reported that prickly pear fruits such as Opuntia ficus-indica have considerable antioxidant, anti-inflammatory, and anti-carcinogenic effects and they have suitable properties for the treatment of obesity and diabetes diseases [ 5 , 13 , 14 , 15 ]. However, regarding Opuntia stricta var.…”

Isolation of N-Ethyl-2-pyrrolidinone-Substituted Flavanols from White Tea Using Centrifugal Countercurrent Chromatography Off-Line ESI-MS Profiling and Semi-Preparative Liquid Chromatography

“…The main advantage of CPC over HPLC is the absence of solid chromatographic phase materials, which allows it to avoid any irreversible adsorption of target compounds to the separation system [ 18 ]. In this study, CPC was implemented in order to take advantage of the complementary liquid–liquid separation characteristics compared to classical solid phase chromatography combined with off-line ESI-MS detection for specific metabolite detection and visualization of co-elution effects in the recovered semi-preparative CPC fractions [ 23 , 24 , 25 ]. The resulting fortified mixture of EPSF compounds was cleaned by size-exclusion chromatography and purified by semi-preparative HPLC to yield the pure epimers R -EGCG-cThea and S -EGCG-cThea.…”