Profiling of phytochemical and antioxidant activity of wild mushrooms: Evidence from the in vitro study and phytoconstituent's binding affinity to the human erythrocyte catalase and human glutathione reductase

Abstract

This study was undertaken to evaluate the appearance of phytochemicals and antioxidant activity of seven wild mushrooms of the University of Chittagong campus. Phytochemical screening was performed using standard methods, whereas DPPH radical scavenging assay was used to elucidate the antioxidant effect. Besides, in silico studies were implemented using the targets of human erythrocyte catalase 3‐amino‐1,2,4‐triazole, human glutathione reductase, and selected compounds. Again, the absorption, distribution, metabolism, elimination and toxicity (ADME/T) analysis has been determined by using online tools. Both Ganoderma lucidum (Curtis) Karst. and Ganoderma applanatum (Pers.) Pat. showed a significant (p < .001) increase in the percentage of scavenging activity at 400 μg/ml concentration when compared with ascorbic acid. The methanol extract of G. lucidum, G. applanatum, and Rhodofomes cajanderi (P. Karst.) B. K. Cui, M. L. Han & Y. C. Dai showed strong antioxidant activity with an IC50 value. In addition, molecular docking studies of the previously isolated compounds from three selective mushrooms revealed that the targeted compounds along with positive controls were able to interact strongly (range: −3.498 to −8.655) with the enzymes. The study concludes that the G. lucidum, G. applanatum, and R. cajanderi mushrooms can be a strong source in the management of oxidative stress‐induced diseases.

Nephroprotective Effects of Two Ganoderma Species Methanolic Extracts in an In Vitro Model of Cisplatin Induced Tubulotoxicity

“…Extracts were then filtered using Büchner filtration, and volumes were then completed at 10 mL. All tests were performed by adapting methods from the literature [ 23 , 24 , 25 ].…”

Interpol Review of Drug Analysis 2019-2022

“…2019 review [ 435 ]; morphological, chemical, and genetic analysis of mycelia of psychedelic fungi collected from a clandestine laboratory using scanning electron microscopy (SEM), mass spectrometry, HRM analysis, and internal transcribed spacer (ITS) sequencing [ 436 ]; 2020 use of TLC, FTIR and GC-MS to differentiate Psilocin (4-hydroxy-N,N-dimethyltryptamine, 4-HO-DMT) and bufotenine (5-hydroxy-N,N-dimethyltryptamine, 5-HO-DMT) and benzene ring regioisomers, 6-hydroxy-N,N-dimethyltryptamine (6-HO-DMT) and 7-hydroxy-N,N-dimethyltryptamine (7-HO-DMT) [ 437 ]; immunochemical detection method for monitoring psilocybin and psilocin in dried powder of hallucinogenic mushroom (Psilocybe cubensis) [ 438 ]; 2021 chemoenzymatic synthesis of 5-methylpsilocin, a novel analogue of psilocybin with potential psychedelic activity [ 439 ]; stability study of psilocybin and its four analogues (psilocin, baeocystin, norbaeocystin, and aeruginascin) in psilocybe cubensis mushrooms [ 440 ]; medicinal properties and bioactive compounds from 79 species of wild mushrooms native to North America [ 441 ]; DNA-based identification of hallucinogenic mushrooms [ 442 ]; forensic detection method for hallucinogenic mushrooms via High-Resolution Melting (HRM) analysis [ 443 ]; 2022 phytochemicals and antioxidant activity of seven wild mushrooms [ 444 ].…”

Assessment of phytochemical screening and antioxidant potential of Heteropogon contortus (L.) whole plant

“…An equilibrium between free radicals as well as antioxidants is essential for the proper physiological functions in the living beings. If free radicals suppress the body's capability to control them, an ailment recognized as oxidative stress results [1,2]. Scientific evidences have projected that in oxidative stress conditions, radicles of oxygen like hydroxyl radical (OH•), peroxyl radicals (H2O2•) and superoxide anions (O 2-) are formed in the biological systems [3].…”