Profiling of bile acids in bovine follicular fluid by fused-core-LC–MS/MS

Sánchez‐Guijo, Alberto; Blaschka, C.; Hartmann, Michaela F.; Wrenzycki, C.; Wudy, Stefan A.

doi:10.1016/j.jsbmb.2016.02.020

Cited by 20 publications

(17 citation statements)

References 26 publications

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“…By interfering with ENO1 , we observed that 12 metabolites from FGCs were significantly downregulated and one metabolite was significantly upregulated. Almost all of these metabolites were found to participate in the growth and development of FGCs, of which indole (Suzen, Cihaner, & Coban, ), vitamin D3 (Bakhshalizadeh et al, ), oleic acid (Sutton‐McDowall et al, ) and cholic acid (Sanchez‐Guijo, Blaschka, Hartmann, Wrenzycki, & Wudy, ) were important for shaping microenvironment development. This analysis of metabolites suggested that ENO1 may affect FGC microenvironment development by regulating the biosynthesis of fatty and unsaturated fatty acids, primary bile acids, phenylalanine, tyrosine and tryptophan.…”

Section: Discussionmentioning

confidence: 99%

“…The decrease in these hormones would cause follicular dysplasia (Kaya et al, ; Sun et al, ). Therefore, the high cholic acid content is an important microenvironment for follicular and FGC development (Sanchez‐Guijo et al, ), while ENO1 promotes the secretion of cholic acid in FGCs, thereby promoting the synthesis of oestrogen and progesterone to shape the microenvironment for FGC development.…”

Section: Discussionmentioning

confidence: 99%

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Metabonomics analysis of Zi goose follicular granulosa cells using ENO1 gene expression interference

Guo

Niu

et al. 2019

Animal Physiology Nutrition

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The Zi goose is native to North‐east China and is noted for its high egg production. Alpha enolase (ENO1) is a glycolytic enzyme which functions as a plasminogen receptor in follicular granulosa cells (FGCs), with several studies showing that FGCs can support follicular development. By transfecting the ENO1 interfering plasmid (shRNA) into FGCs, ENO1 expression in these cells was downregulated, suggesting the successful knock‐down of ENO1 in these cells. In this knock‐down model, we detected 13 metabolites from FGCs using LC/MS. When compared with the non‐coding shRNA (NC) group, the lower level metabolites were (R)‐(+)‐citronellic acid, altretamine, 3‐hydroxycaproic acid, heptadecanoic acid, cholecalciferol vitamin D3, indole, benzoic acid, capric acid, caffeic acid, azelaic acid, 3,4‐dihydroxyhydrocinnamic acid and cholic acid, while oleic acid was detected at high levels. To further examine the results of metabolomics, six key metabolites were verified by gas chromatography‐mass spectrometry (GC‐MS). We found that vitamin D3, indole, benzoic acid, capric acid and cholic acid were significantly downregulated in the shRNA group, while oleic acid was significantly upregulated. This observation was consistent with the metabolomics data. Through these studies, we found that decreased ENO1 levels altered certain metabolite levels in FGCs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Metabonomics analysis of Zi goose follicular granulosa cells using ENO1 gene expression interference

Guo

Niu

et al. 2019

Animal Physiology Nutrition

View full text Add to dashboard Cite

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“…The sample preparation and the BA quantification followed the procedure outlined by Sanchez‐Guijo, Blaschka, et al (). Biological fluid (200 µl) was incubated after the addition of deuterated internal standards with gently shaking for 30 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…BAs are present in several body fluids like bile, blood and urine. Recently, BAs were also detected in follicular fluid (FF) of humans (Nagy et al, ; Smith et al, ) and cattle (Sanchez et al, ; Sanchez‐Guijo, Blaschka, Hartmann, Wrenzycki, & Wudy, ). Smith et al () determined the total BA concentration in human FF depending on an hCG (human chorionic gonadotropin) treatment.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, different BA subspecies have been quantified in FF derived from follicles of different sizes (3–5 mm, 6–8 mm, 9–14 mm, ≥15 mm) collected from abattoir‐derived ovaries during interoestrus. The primary BA CA and the corresponding conjugated form GCA showed the highest concentrations irrespective of the follicle size (Sanchez‐Guijo, Blaschka, et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Profile of bile acid subspecies is similar in blood and follicular fluid of cattle

Blaschka

Sánchez‐Guijo

Wudy

et al. 2019

Veterinary Medicine & Sci

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The composition of follicular fluid (FF) has an impact on the developmental capacity of the oocyte and the resulting embryo. FF is composed of blood plasma constituents which cross the blood follicular barrier and the secretory components of granulosa and theca cells. Moreover, it has been shown recently that follicular cells have the ability to synthesize bile acids (BAs). BAs are present in several fluids of mammals especially in bile, blood and urine. FF is an essential impacting factor on the oocyte quality and therefore resulting embryos. To achieve a better understanding of this subject, the presence and concentration of BAs were measured in fluid collected from bovine follicles, categorized according to their size, throughout two entire oestrus cycles and compared to those in blood and urine. The body fluids were collected during the same examination procedure and in total samples from four heifers were obtained. A broad spectrum of 11 BA derivatives was measured applying liquid chromatography–tandem mass spectrometry (LC‐MS/MS). The simultaneous and direct quantification of BAs in different body fluids of cattle are reported. Within the follicular fluid, blood and urine, cholic acid and glycocholic acid are the dominant BA subspecies irrespective of the oestrus cycle stage. Moreover, BA concentrations in blood compared to those in the FF were similar. For the first time these results clearly highlight the presence of different BA subspecies in FF, blood and urine during the oestrus cycle in cattle.

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In-vitro-Produktion von Nutztier-Embryonen

Wrenzycki

2023

Biotechnologie Bei Nutztieren 1

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Profiling of bile acids in bovine follicular fluid by fused-core-LC–MS/MS

Cited by 20 publications

References 26 publications

Metabonomics analysis of Zi goose follicular granulosa cells using ENO1 gene expression interference

Metabonomics analysis of Zi goose follicular granulosa cells using ENO1 gene expression interference

Profile of bile acid subspecies is similar in blood and follicular fluid of cattle

In-vitro-Produktion von Nutztier-Embryonen

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