Profile of bile acid subspecies is similar in blood and follicular fluid of cattle

Abstract

The composition of follicular fluid (FF) has an impact on the developmental capacity of the oocyte and the resulting embryo. FF is composed of blood plasma constituents which cross the blood follicular barrier and the secretory components of granulosa and theca cells. Moreover, it has been shown recently that follicular cells have the ability to synthesize bile acids (BAs). BAs are present in several fluids of mammals especially in bile, blood and urine. FF is an essential impacting factor on the oocyte quality and therefore resulting embryos. To achieve a better understanding of this subject, the presence and concentration of BAs were measured in fluid collected from bovine follicles, categorized according to their size, throughout two entire oestrus cycles and compared to those in blood and urine. The body fluids were collected during the same examination procedure and in total samples from four heifers were obtained. A broad spectrum of 11 BA derivatives was measured applying liquid chromatography–tandem mass spectrometry (LC‐MS/MS). The simultaneous and direct quantification of BAs in different body fluids of cattle are reported. Within the follicular fluid, blood and urine, cholic acid and glycocholic acid are the dominant BA subspecies irrespective of the oestrus cycle stage. Moreover, BA concentrations in blood compared to those in the FF were similar. For the first time these results clearly highlight the presence of different BA subspecies in FF, blood and urine during the oestrus cycle in cattle.

Serum Metabolomic Analysis of Synchronous Estrus in Yaks Based on UPLC-Q-TOF MS Technology

“…This association underscores the potential significance of BAs in modulating reproductive outcomes [34,35]. Blaschka et al [36] discovered that cholic acid and glycocholic acid are the predominant bile acid species within follicular fluid, blood, and urine across all stages of the estrus cycle. Cholic acid, a free bile acid, is prominently found in the bile of mammals and other vertebrates.…”

Non-invasive metabolomics biomarkers of production efficiency and beef carcass quality traits

“…Urine steroids and BA are cholesterol-derived metabolites involved in the regulation of different biological pathways mainly in metabolic homeostasis and maintenance 26 . Total BA concentration is traditionally used as a clinical index of liver pathology in cattle 27 , although the advance of current quantification techniques of BA and steroid profiling makes it possible to elucidate their roles in diet intervention 28 , breeding 29 and metabolic functions 30 . Here a total 18 BAs and 5 steroids were detected in cattle urine, with conjugated-BAs positively associated with sex-steroids and glucocorticoids in both diets.…”

Non-Invasive Metabolomics Biomarkers of Production Efficiency and Beef Carcass Quality Traits

“…Urine steroids and BA are cholesterol-derived metabolites involved in the regulation of different biological pathways mainly in metabolic homeostasis and maintenance 26 . Total BA concentration is traditionally used as a clinical index of liver pathology in cattle 27 , although the advance of current quanti cation techniques of BA and steroid pro ling makes it possible to elucidate their roles in diet intervention 28 , breeding 29 and metabolic functions 30 . Here a total 18 BAs and 5 steroids were detected in cattle urine, with conjugated-BAs positively associated with sex-steroids and glucocorticoids in both diets.…”