Profiling of adhesive properties of the agglutinin-like sequence (ALS) protein family, a virulent attribute of<i>Candida albicans</i>

Aoki, Wataru; Kitahara, Nao; Miura, Naruhisa; Morisaka, Hironobu; Kuroda, Kouichi; Ueda, Mitsuyoshi

doi:10.1111/j.1574-695x.2012.00941.x

Cited by 21 publications

(18 citation statements)

References 9 publications

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“…Several cell surface adhesins such as those belonging to the agglutinin-like sequence (ALS) family increase yeast aggregation and adhesion to epithelia (Dranginis et al, 2007; Aoki et al, 2012). Beaussart et al (2012) postulated that the hydrophobicity of hyphae depends on enhanced exposure of Als3 protein on the surface and causes stronger C. albicans adhesion to hydrophobic substrata (Table 1).…”

Section: Are Hydrophobic Microorganisms More Pathogenic?mentioning

confidence: 99%

How microorganisms use hydrophobicity and what does this mean for human needs?

Krasowska

Sigler

2014

Front. Cell. Infect. Microbiol.

475

307

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Cell surface hydrophobicity (CSH) plays a crucial role in the attachment to, or detachment from the surfaces. The influence of CSH on adhesion of microorganisms to biotic and abiotic surfaces in medicine as well as in bioremediation and fermentation industry has both negative and positive aspects. Hydrophobic microorganisms cause the damage of surfaces by biofilm formation; on the other hand, they can readily accumulate on organic pollutants and decompose them. Hydrophilic microorganisms also play a considerable role in removing organic wastes from the environment because of their high resistance to hydrophobic chemicals. Despite the many studies on the environmental and metabolic factors affecting CSH, the knowledge of this subject is still scanty and is in most cases limited to observing the impact of hydrophobicity on adhesion, aggregation or flocculation. The future of research seems to lie in finding a way to managing the microbial adhesion process, perhaps by steering cell hydrophobicity.

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Section: Are Hydrophobic Microorganisms More Pathogenic?mentioning

confidence: 99%

How microorganisms use hydrophobicity and what does this mean for human needs?

Krasowska

Sigler

2014

Front. Cell. Infect. Microbiol.

475

307

View full text Add to dashboard Cite

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“…The AMP may also protect normal microflora, resulting in enhanced safety. Conjunction of additional selective domains that target other virulent attribute of C. albicans may further increase specificity and activity [162,163,164,165]. …”

Section: Design Of Molecular-targeted Ampsmentioning

confidence: 99%

Characterization of Antimicrobial Peptides toward the Development of Novel Antibiotics

2013

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Antimicrobial agents have eradicated many infectious diseases and significantly improved our living environment. However, abuse of antimicrobial agents has accelerated the emergence of multidrug-resistant microorganisms, and there is an urgent need for novel antibiotics. Antimicrobial peptides (AMPs) have attracted attention as a novel class of antimicrobial agents because AMPs efficiently kill a wide range of species, including bacteria, fungi, and viruses, via a novel mechanism of action. In addition, they are effective against pathogens that are resistant to almost all conventional antibiotics. AMPs have promising properties; they directly disrupt the functions of cellular membranes and nucleic acids, and the rate of appearance of AMP-resistant strains is very low. However, as pharmaceuticals, AMPs exhibit unfavorable properties, such as instability, hemolytic activity, high cost of production, salt sensitivity, and a broad spectrum of activity. Therefore, it is vital to improve these properties to develop novel AMP treatments. Here, we have reviewed the basic biochemical properties of AMPs and the recent strategies used to modulate these properties of AMPs to enhance their safety.

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“…The representative virulent attributes of C. albicans include the secreted aspartic protease family (Schaller et al ., ; Aoki et al ., , , b), the agglutinin‐like sequence family (Phan et al ., ; Almeida et al ., ; Hoyer et al ., ; Aoki et al ., , b), and yeast‐to‐hyphal transition (Lo et al ., ; Whiteway & Bachewich, ). Using these attributes, C. albicans binds to mammalian cells including human cells, invades epithelial tissues, and disseminate via blood stream.…”

Section: Introductionmentioning

confidence: 99%

Time-course proteomic profile ofCandida albicansduring adaptation to a fetal serum

et al. 2012

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Candida albicans is a commensal organism; however, it causes fatal diseases if the host immunity is compromised. The mortality rate is very high due to the lack of effective treatment, leading to ceaseless demand for novel pharmaceuticals. In this study, time-course proteomics of C. albicans during adaptation to fetal bovine serum (FBS) was described. Time-course proteomics is a promising way to understand the exact process of going adaptation in dynamically changing environments. Candida albicans was cultivated in yeast nitrogen base (YNB) ± FBS media, and we identified 1418 proteins in the endpoint samples incubated for 0 or 60 min by a LC-MS/MS system with a long monolithic silica capillary column. Next, we carried out time-course proteomics of the YNB + FBS samples to identify top-priority proteins for adaption to FBS. We identified 16 proteins as nascent/newly synthesized proteins, and they were recognized as candidates of important virulent factors. Gene ontology analysis revealed that transport-related proteins were enriched in the 16 proteins, indicating that C. albicans probably put priority in time on the acquisition of essential elements. Time-course proteomics of C. albicans revealed the order of priority to adapt to FBS. Depicting time-course dynamics will lead to profound understandings of virulence of C. albicans.

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Profiling of adhesive properties of the agglutinin-like sequence (ALS) protein family, a virulent attribute ofCandida albicans

Cited by 21 publications

References 9 publications

How microorganisms use hydrophobicity and what does this mean for human needs?

How microorganisms use hydrophobicity and what does this mean for human needs?

Characterization of Antimicrobial Peptides toward the Development of Novel Antibiotics

Time-course proteomic profile ofCandida albicansduring adaptation to a fetal serum

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