Profiling and Pharmacokinetic Studies of Alkaloids in Rats After Oral Administration of Zanthoxylum nitidum Decoction by UPLC-Q-TOF-MS/MS and HPLC-MS/MS

Abstract

Zanthoxylum nitidum (Roxb.) DC (Rutaceae), called as “liangmianzhen” in China, is well known for its anti-inflammation and analgesic effect. Alkaloids are its main active constituents. However, little has been known about the absorption of main alkaloids in vivo. In this study, an ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry was employed for identification of absorbed alkaloids in rats after oral administration of Z. nitidum decoction. By analyzing the fragmentation patterns, a total of nineteen alkaloids were exactly or tentatively identified in rat plasma after treatment, of which magnoflorine, α-allocryptopine, and skimmianine are dominant. Moreover, a high performance liquid chromatography coupled mass spectrometry method was developed for simultaneous quantification of magnoflorine, α-allocryptopine, and skimmianine, and successfully applied to pharmacokinetic study in rats after oral administration of Z. nitidum decoction. The research would contribute to comprehensive understanding of the material basis and function mechanism of Z. nitidum decoction.

Phytochemical Analysis and Anti-Ascaris suum Activity of Different Zanthoxylum Species In Vitro and In Vivo

“…Generally, the highest level of confidential annotation is currently based on matching two or more orthogonal properties of an authentic standard substance performed under consistent LC-HRMS conditions, such as retention time (RT), accurate mass (AM), optimal collision energy (OCE), and tandem mass spectrometry (MS/MS) information. Therefore, the general identification criteria (RT-AM-MS/MS) applied previously by adding the auxiliary ion ratio (IR) proposed in the current study could be integrated to improve the credibility of isomeric annotation. ,, The characteristic compounds in methanol extracts of different plants were accurately identified, and the compounds in the extracts of ChuanJiao with different solvents were identified accurately (Table ).…”

“…Therefore, the general identification criteria (RT-AM-MS/MS) applied previously by adding the auxiliary ion ratio (IR) proposed in the current study could be integrated to improve the credibility of isomeric annotation. 19,22,23 The 1). Method Validation In Vivo.…”

Liquid chromatography‐mass spectrometry‐based strategy for systematic profiling of chemical components and associated quantitative analysis of quality markers in Qi‐Wei‐Tong‐Bi oral liquid

“…The typical loss of CH 3 group also produced two fragmentation ions at m/z 282.0913 [M−(CH 3 ) 2 NH−CH 3 ] + and 222.0665 [M−(CH 3 ) 2 NH−CH 3 OH−CO−CH 3 ] + . By its characteristic fragmentation pattern, the published literature, and comparison with its reference standard, C59 was identified as magnoflorine [10, 11]. Its MS/MS spectrum and proposed fragmentation pathway are demonstrated in Figure S1.…”

“…Figure 2 . By its characteristic fragmentation pattern, the published literature, and comparison with its reference standard, C59 was identified as magnoflorine [10,11]. Its MS/MS spectrum and proposed fragmentation pathway are demonstrated in Figure S1.…”

The Nutritional Properties, Chemical Compositions, and Functional Characteristics of the Aerial Parts of Adonis coerulea

“…Among the identified compounds, most of the compounds belong to flavonoids, including licochalcone A, isoorientin, orientin, luteolin-5-glucuronide, 5 -hydroxypuerarin, vitexin, norwogonin-7-O-glucuronide, 3-rha-7-rha-quercetin, and acacetin-7-O-rutinoside (32); one amino acid, phenylalanine (33), three fatty acids, caprylic acid, linolenic acid, and linoelaidic acid (34,35) were also detected from ACAP. In addition, one alkaloid magnoflorine (36), one terpenoid bryoamaride (37), one cardiac aglycone strophanthidin (38), and one coumarins 4-methylumbelliferyl glucuronide (39) were identified from this species. Isoorientin, orientin, and strophanthidin were also found by previous reports (38,40).…”