Profiles of Steroidal Saponins from the Aerial Parts of Tribulus pentandrus, T. megistopterus subsp. pterocarpus and T. parvispinus by LC‐ESI‐MS/MS

Hamed, Arafa I.; Janda, Bogdan; Mahalel, Usama A.; Stochmal, Anna; Oleszek, Wiesław

doi:10.1002/pca.2363

Cited by 14 publications

(7 citation statements)

References 16 publications

(48 reference statements)

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“…The LCQ mass spectrometer was run sequentially. Full-scan MS was performed to ac-quire analytical data on anions within the scan range, followed by an MS/MS trial at a normalized collision energy of 35% to detect the most notable [M-H] − ion that was obtained in the first step (Hamed et al, 2012(Hamed et al, , 2016(Hamed et al, , 2017Ito et al, 2013;Hsu, 2016).…”

Section: Hplc and Esi-ms Of Glycosphingolipids Lps And Oligosaccharmentioning

confidence: 99%

Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma

Hamed

Said

Kontek

et al. 2020

Journal of Dairy Science

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The aim of this study was to characterize minor lipids in methanol fraction extracted from raw camel milk after loading it on a water-preconditioned short C18 open column and fractionating with a gradient of methanol/water. The C18 column showed high fractionation efficiency of minor lipids, such as glycosphingolipids, lipopolysaccharides, or oligosaccharides, when compared with other constituents, in particular polysaccharides, proteins, and free fatty acids. Liquid chromatography electrospray ionization tandem mass spectrometry in negative ion mode was used to identify 21 new glycosphingolipids, lipopolysaccharides, and oligosaccharides. Electrospray ionization tandem mass spectrometry was qualified to provide relevant data for recognizing the molecular mass, glycosylation sequences, and structure of saccharide moieties for the revealed compounds. The sequence of combinations of one selected lipopolysaccharide, which was considered the backbone of the remaining lipopolysaccharides, was confirmed in a density functional theory study. The obtained results showed that the tested fraction is a rich source of glycosphingolipids, lipopolysaccharides, and oligosaccharides with antioxidant activity.

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Section: Hplc and Esi-ms Of Glycosphingolipids Lps And Oligosaccharmentioning

confidence: 99%

Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma

Hamed

Said

Kontek

et al. 2020

Journal of Dairy Science

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“…Comparison of chromatographic behavior of detected glycosides with literature data corroborates these conclusions. Elution of steroidal glycosides from the reversed-phase mainly depends on aglycone structure [12,14]; irrespective of the structure of carbohydrate chains, derivatives containing hydroxyl groups in the cyclic part of molecule (derivatives of hydroxy-diosgenin and gitogenin/neogitogenin) are eluted from the column before unsubsti- Detector signal, relative intensity, % tuted compounds (derivatives of tigogenin/neotigogenin).…”

Section: Resultsmentioning

confidence: 99%

Effect of Synthetic Auxin Analogs (2.4-D and α-NAA) on Growth and Biosynthetic Characteristics of Suspension Cell Culture of Tribulus terrestris L.

Tomilova

Ханды

Кочкин

et al. 2020

Russ J Plant Physiol

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Effect of synthetic analogs of auxins-2.4-dichlorophenoxyacetic (2.4-D) and α-naphthylacetic (α-NAA) acids-on growth characteristics and accumulation of steroidal glycosides was investigated in suspension cell culture of Tribulus terrestris L. It was found that the substitution of α-NAA for 2.4-D in the nutrient medium brought about a rise in the content of steroidal glycosides in the cultured cells (up to six times) and broadened their structural diversity (nine identified oligofurostanosides when growing culture on the medium with α-NAA vs. five compounds on the medium with 2.4-D). Positive influence of α-NAA exerted on biosynthetic characteristics of T. terrestris suspension cell culture was accompanied by changes in cell morphology (cytodifferentiation), the extent of their aggregation, and gradual decrease (during 3-4 cycles of growing) in culture viability up to its extinction. Simultaneous presence of both synthetic analogs of auxins (α-NAA and 2.4-D) in the growing medium also caused a rise in the content of steroidal glycosides in the cells of T. terrestris, although it was less pronounced (up to three times); however, the culture showed a relatively steady growth and great viability in this case. It was concluded that these two auxin analogs differently influenced the growth of plants' cells in vitro and biosynthesis of substances of specialized metabolism, 2.4-D promoted cell proliferation, whereas α-NAA induced cytodifferentiation and activated the production of secondary compounds. This conclusion is corroborated by the data concerning plant cell cultures of other species of medicinal plants accumulating secondary metabolites from other groups. For instance, in the experiments with suspension cell culture of Panax ginseng C.A. Mey., substitution in the growth medium of α-NAA for 2.4-D brought about a rise in the content and diversity of triterpene glycosides (ginsenosides) associated with an elevated cell aggregation and deterioration of culture's viability. Comparison of production of steroidal glycosides in plant cell cultures of T. terrestris and Dioscorea deltoidea Wall. that is grown for more than 40 years has shown that both cultures accumulated only furostanol (rather than spirostanol) glycosides that promoted cell proliferation. In suspension cell culture of Dioscorea, a high level of oligofurostanosides (up to 12% of dry biomass) was accumulated when growing culture on the medium with 2.4-D. Thus, investigation into the production of furostanol glycosides in plant cultured cells points to a multifactor system of regulation of secondary metabolism in vitro. Different synthetic auxin analogs may exert alternative influences on growth and biosynthetic processes. At the same time, prolonged culturing leads to an autoselection of cells with the properties promoting proliferation, specifically, with a high content of furostanol glycosides.

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“…Furthermore, Hamid et al observed that native steroidal glycosides of both T. pentandrus and T. megistopterus subsp. pterocarpus were very similar to each other, but that steroidal glycosides from T. parvispinus was remarkably different [ 71 ]. The authors elucidated the 10 chemical structures ( 104 – 113 ) of the related aglycones that have been reported from the genus Tribulus [ 71 ], meanwhile Mahalel isolated the steroidal glycoside pentandroside A ( 114 ) from the aerial parts of T. pentandrus and evaluated its antimicrobial activity [ 72 ].…”

Section: Thymelaeaceae and Tribulaceaementioning

confidence: 99%

The Chemistry and Biological Activities of Natural Products from Northern African Plant Families: From Taccaceae to Zygophyllaceae

Ntie‐Kang

Njume

Malange

et al. 2016

Nat. Prod. Bioprospect.

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Traditional medicinal practices have a profound influence on the daily lives of people living in developing countries, particularly in Africa, since the populations cannot generally afford the cost of Western medicines. We have undertaken to investigate the correlation between the uses of plants in Traditional African medicine and the biological activities of the derived natural products, with the aim to validate the use of traditional medicine in Northern African communities. The literature is covered for the period 1959–2015 and part III of this review series focuses on plant families with names beginning with letters T to Z. The authors have focused on curating data from journals in natural products and phytomedicine. Within each journal home page, a query search based on country name was conducted. All articles “hits” were then verified, one at a time, that the species was harvested within the Northern African geographical regions. The current data partly constitutes the bases for the development of the Northern African natural compounds database. The review discusses 284 plant-based natural compounds from 34 species and 11 families. It was observed that the ethnobotanical uses of less than 40 % of the plant species surveyed correlated with the bioactivities of compounds identified.Graphical AbstractElectronic supplementary materialThe online version of this article (doi:10.1007/s13659-016-0091-9) contains supplementary material, which is available to authorized users.

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Profiles of Steroidal Saponins from the Aerial Parts of Tribulus pentandrus, T. megistopterus subsp. pterocarpus and T. parvispinus by LC‐ESI‐MS/MS

Abstract: HPLC-ESI-MS/MS allowed identification of the key compounds without preparative isolation of the components from the crude extract of Tribulus species.

Cited by 14 publications

References 16 publications

Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma

Electrospray ionization mass spectrometry characterization of ubiquitous minor lipids and oligosaccharides in milk of the camel (Camelus dromedarius) and their inhibition of oxidative stress in human plasma

Effect of Synthetic Auxin Analogs (2.4-D and α-NAA) on Growth and Biosynthetic Characteristics of Suspension Cell Culture of Tribulus terrestris L.

The Chemistry and Biological Activities of Natural Products from Northern African Plant Families: From Taccaceae to Zygophyllaceae

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