Profiles of mitochondrial DNA methylation in the ridgetail white prawn, Exopalaemon carinicauda (Holthuis, 1950) (Decapoda, Palaemonidae) under heavy metal stress from chromium and cadmium

Abstract

For the purpose of studying the epigenetic characteristics of the mitochondrial genome of the ridgetail white prawn, Exopalaemon carinicauda (Holthuis, 1950) under Cd2+ and Cr6+ heavy metal stress, the mitochondrial DNA methylation of E. carinicauda was analysed by bisulfite sequencing PCR (BSP). Many methylation sites were found at the 3′ end sequence of COX3 and at the starting region sequence of ND3, while only a few methylation sites were found at the 3′ end sequence of ND5. The mitochondrial genome was inferred to regulate the energy metabolism through the methylation process. In addition, under Cd2+ stress, mitochondrial DNA methylation was more common, and found during all stress periods (3, 6, 12, 24, 48, 72 and 96 h), while under Cr6+ stress, mitochondrial DNA methylation was less common, mainly occurring after 48 hours of stress. The sensitivity of the mitochondrial genome response to Cd2+ stress was inferred to be greater than that to Cr6+. This study revealed for the first time that methylation occurs in the mitochondrial genome of E. carinicauda in response to heavy metal stress.

Perspectives of Genetic Damage and Epigenetic Alterations by Hexavalent Chromium: Time Evolution Based on a Bibliometric Analysis

“…The mitochondrial genome can regulate energy metabolism through the methylation process. Mitochondrial DNA methylation was more common under exposure to Cd­(II) than that under Cr­(VI) …”

“…Mitochondrial DNA methylation was more common under exposure to Cd(II) than that under Cr(VI). 70 Post-translational modifications of histone affect specific gene expression by the action of enzymes such as histone methyltransferases, demethylases, histone acetyltransferases, and deacetylases directly. The exposure of human lung A549 cells to 5 or 10 μM Cr(VI) for 1 h resulted in the increase of global level of dimethylated histone H3 lysine 9 (H3K9), rich in the human MLH1 gene (a tumor suppressor gene) promoter, as well as the protein and mRNA levels of G9a, a histone methyltransferase specifically methylating H3K9, followed by the decrease of MLH1 mRNA expression.…”

Impact of Ocean Acidification on the Gut Histopathology and Intestinal Microflora of Exopalaemon carinicauda

“…carinicauda is a commercially important shrimp species, as compared with other species; it possesses distinct advantages in terms of fast growth, short breeding cycle, and strong adaptability. Such features also make it a suitable organism for research on crustacean biology [20][21][22][23]. Indeed, as a model species of marine crustaceans, E. carinicauda is often used in the study of environmental stress, such as salinity [24] and temperature [25].…”

Cloning and function of the cyclin‐dependent kinase 9 gene in ovarian development of the ridgetail white prawn, Exopalaemon carinicauda

“…Exopalaemon Carinicauda (Arthropoda, Crustacea, Decapoda and Exopalaemon) is a shrimp of economic importance in China, covering an area of about 20,000 hectares. The annual output is about 50,000 tons (Deng et al, 2021;Ma et al, 2019;Wang et al, 2021).…”

“…Exopalaemon Carinicauda (Arthropoda, Crustacea, Decapoda and Exopalaemon ) is a shrimp of economic importance in China, covering an area of about 20,000 hectares. The annual output is about 50,000 tons (Deng et al, 2021; Ma et al, 2019; Wang et al, 2021). At present, the cultured seedlings of this shrimp are mainly obtained by collecting shrimp seedlings from natural sea areas or by the self‐propagation of female parents with eggs (Li et al, 2019; Liang et al, 2020).…”