Profiles of differentially expressed circRNAs in esophageal and breast cancer

Shi, Peiyi; Sun, Jian; He, Biyu; Song, Huan; Li, Zhongqi; Kong, Wei­min; Wang, Jianping; Wang, Jianming; Xue, Hengchuan

doi:10.2147/cmar.s167863

Cited by 33 publications

(37 citation statements)

References 40 publications

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“…Recently, accumulating evidence has indicated that circRNAs could function as a biomarker to predict the clinical severity in cancer patients [17][18][19][20]. Previously, circ_0005230 was found upregulated in BC tissues revealed by the human circRNA microarray [13]. However, its expression profiles in BC cells, clinical features, biological functions, and mechanisms were not explored yet.…”

Section: Discussionmentioning

confidence: 99%

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Increased Expression of Circular RNA circ_0005230 Indicates Dismal Prognosis in Breast Cancer and Regulates Cell Proliferation and Invasion via miR-618/ CBX8 Signal Pathway

Yao

Leng

et al. 2018

Cell Physiol Biochem

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Background/Aims: Circular RNAs (circRNAs) are a class of non-coding RNAs. They have been proved to be critically involved in tumorigenesis and progression of malignancies through competing endogenous RNA (ceRNA) mechanism. Nevertheless, the exploration between circRNAs and pathogenesis of breast cancer (BC) is limited. Previously, circ_0005230 was identified upregulated in BC tissues screened by circRNA microarray. In the present study, we aimed to investigate the expression pattern, functional role, and mechanism of circ_0005230 in BC. Methods: qRT-PCR was conducted to elucidate the expression levels of circ_0005230 in BC tissues and cells. Additionally, the clinical severity and prognostic value were investigated. CCK-8, colony-forming, flow cytometric assays were performed. Animal study was conducted to validate the in vitro data. What’s more, Transwell assays were induced to detect the cell metastatic properties of circ_0005230 exerts in BC cells. Luciferase reporter assay was used to measure the mechanism of circ_0005230. Results: circ_0005230 was overexpressed in BC tissue specimens and cell lines. The overexpression of circ_0005230 was related to adverse phenotypes in the patients with BC. In addition, circ_0005230 could be regarded as a prognostic predictor in BC patients. In vitro and in vivo data demonstrated the cell growth promoting role of circ_0005230. Moreover, circ_0005230 could also promote cell migratory and invasive capacities. For the mechanism investigation, circ_0005230 was proved to be a sponge of miR-618, and expression of miR-618 could regulate CBX8 expression via targeting the 3’UTR of CBX8. Rescue assays also illustrated an oncogenic function of circ_0005230 in BC via acting as a miR-618 sponge to promote CBX8 expression. Conclusion: circ_0005230/miR-618/CBX8 axis might play a key role in BC tumorigenesis and development.

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Section: Discussionmentioning

confidence: 99%

“…Previously, Shi et al reported that circ_0005230 expression was dramatically elevated in four paired BC tissue samples relative to their corresponding normal tissue samples screened by circRNA microarray [13]. Circ_0005230 was spliced from DNM3OS (DNM3 opposite strand/antisense RNA) gene.…”

Section: Introductionmentioning

confidence: 99%

Increased Expression of Circular RNA circ_0005230 Indicates Dismal Prognosis in Breast Cancer and Regulates Cell Proliferation and Invasion via miR-618/ CBX8 Signal Pathway

Yao

Leng

et al. 2018

Cell Physiol Biochem

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“…However, the research regarding circRNAs in BC is just beginning. Previous study uncovered that circ_0103552 level was significantly boosted in tissue specimens compared to the noncancerous tissue samples proved by circRNA microarray . circ_0103552 is a circRNA mapped to chr15:43294752‐43314999 in human genome.…”

Section: Introductionmentioning

confidence: 99%

Circular RNA circ_0103552 forecasts dismal prognosis and promotes breast cancer cell proliferation and invasion by sponging miR‐1236

Yang

Song

Chen

et al. 2019

J of Cellular Biochemistry

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Breast cancer (BC) is a fatal malignancy, which has a high incidence and mortality across the world. Circular RNAs (circRNAs) have crucial roles in tumor initiation and progression. In this project, a newly found circRNA, circ_0103552 was explored. Quantitative real‐time polymerase chain reaction was applied to measure its expression in the patients' tissue specimens and cells. In addition, Fisher's exact test, Kaplan‐Meier curves and Cox regression model were conducted to discover the clinical significance of circ_0103552. Cell viability, clone‐forming ability, apoptosis, and metastatic properties of BC cells were evaluated by gain/loss‐of‐function assays. Dual luciferase reporter assay was applied to illuminate its mechanisms. As data indicated, circ_0103552 was significantly elevated in BC tissue samples and cells. In addition, its expression correlates with clinical severity and dismal prognosis. Furthermore, circ_0103552 could facilitate cell growth, clone‐forming ability, migration and invasion, and decrease apoptotic cells. For the mechanism exploration, circ_0103552 could directly sponge miR‐1236 to execute oncogenic activities in BC cells. In summary, this study provides a novel circRNA in tumorigeneis and progression of BC and may help to develop effective therapeutic target for this devastating disease.

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“…1 With the rapid development of high-throughput sequencing technology and bioinformatics, accumulating evidence has demonstrated that ncRNAs are involved in the pathophysiology of various diseases, including Alzheimer's disease, 2 cardiovascular disease, 3 and cancers. [4][5][6][7][8] Abundant and functionally important types of ncRNAs include Dovepress Dovepress 1998 song et al tRNAs, rRNAs, miRNAs, siRNAs, lncRNAs, circRNAs, etc. ncRNAs play an important role in the epigenetic regulation of gene expression.…”

Section: Introductionmentioning

confidence: 99%

“…In the current study, we had a great interest in a candidate circRNA (hsa_circ_0000337, also known as hsa_cir-cRNA_100872) in ESCC, which was identified through a high-throughput Arraystar Human circRNA Microarray. 8 hsa_circ_0000337 was one of the top ten upregulated cir-cRNAs with a fold change (FC) =6.255 and P=0.006. To evaluate the feasibility of hsa_circ_0000337 as a diagnostic biomarker and therapeutic target, we verified its expression in ESCC tissues and cell lines and explored the functions of this specific circRNA in vitro by Cell Counting Kit-8 (CCK-8), colony formation, wound healing, and Transwell assays.…”

Section: Introductionmentioning

confidence: 99%

<p>Upregulated circ RNA hsa_circ_0000337 promotes cell proliferation, migration, and invasion of esophageal squamous cell carcinoma</p>

Song¹,

Xu²,

Shi³

et al. 2019

CMAR

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Background As a new class of endogenous ncRNAs, circRNAs have been recently verified to be involved in the carcinogenesis and progression of human cancers. In the current study, we attempted to explore the potential function of a candidate circRNA (hsa_circ_0000337) in esophageal squamous cell carcinoma (ESCC). Patients and methods The altered expression of hsa_circ_0000337 was validated in clinical samples from 48 patients with ESCC. The human esophageal carcinoma cell lines KYSE-150 and TE-1, and the normal human esophageal epithelial cell line (HET-1A) were applied for functional analysis of hsa_circ_0000337. Cell proliferation was measured using the Cell Counting Kit-8 assay and the colony formation assay. Cell invasion and migration were detected by Transwell and wound healing assays, respectively. We further performed bioinformatic analysis and luciferase reporter assays to explore the role of hsa_circ_0000337 as a miRNA sponge. Results hsa_circ_0000337 was significantly upregulated in ESCC tissues compared to adjacent normal-appearing tissues ( P <0.0001). In our in vitro experiment, the expression of hsa_circ_0000337 was higher in TE-1 compared to the normal human esophageal epithelial cell line HET-1A ( P <0.001), but was not significantly different in KYSE-150 ( P >0.05). Knockdown of hsa_circ_0000337 significantly inhibited cell proliferation, migration, and invasion in TE-1 and KYSE-150 cell lines. Bioinformatics predicted and luciferase reporter assay verified that hsa_circ_0000337 could bind to miR-670-5p, a ncRNA involved in carcinogenesis. It is estimated that 21 genes are regulated by miR-670-5p. Conclusion hsa_circ_0000337 was found to be an upregulated circRNA that is related to ESCC and promotes the progression of disease by regulating cell proliferation, migration, and invasion. These findings suggest that this circRNA could be a promising diagnostic biomarker and potential therapeutic target.

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Profiles of differentially expressed circRNAs in esophageal and breast cancer

Cited by 33 publications

References 40 publications

Increased Expression of Circular RNA circ_0005230 Indicates Dismal Prognosis in Breast Cancer and Regulates Cell Proliferation and Invasion via miR-618/ CBX8 Signal Pathway

Increased Expression of Circular RNA circ_0005230 Indicates Dismal Prognosis in Breast Cancer and Regulates Cell Proliferation and Invasion via miR-618/ CBX8 Signal Pathway

Circular RNA circ_0103552 forecasts dismal prognosis and promotes breast cancer cell proliferation and invasion by sponging miR‐1236

<p>Upregulated circ RNA hsa_circ_0000337 promotes cell proliferation, migration, and invasion of esophageal squamous cell carcinoma</p>

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