Profile of RT-PCR for SARS-CoV-2: A Preliminary Study From 56 COVID-19 Patients

Xiao, Ai Tang; Tong, Yi Xin; Zhang, Sheng

doi:10.1093/cid/ciaa460

Cited by 237 publications

(276 citation statements)

References 13 publications

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“…The average VSPs were similar to those reported in the previous study [32]. After the multivariate linear regression analysis, we identi ed that disease durations at admission and HCQ/CQ treatment were independent parameters related to patients' VSPs, indicating patients might get better prognosis if being well treated earlier.…”

Section: Discussionsupporting

confidence: 83%

Hydroxychloroquine/ Chloroquine in Patients With COVID-19 in Wuhan, China: A Retrospective Study

Chen

Liu

Cheng

et al. 2020

Preprint

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Background: We aim to evaluate the treatment value and safety of hydroxychloroquine (HCQ)/ chloroquine (CQ) in COVID-19.Methods: We retrospectively reviewed the medical charts of patients with COVID-19 admitted to an inpatient ward in Wuhan from 2020. 02. 08 to 2020. 03. 05. Patients with HCQ/ CQ and age, gender, disease severity matched ones without HCQ/ CQ were selected at a 1:2 ratio. The clinical, laboratory and imaging findings were compared between these two groups. The multivariate linear regression analysis was performed to identify the factors that might influence patients’ virus shedding periods (VSPs).Results: A total of 14 patients with HCQ/ CQ and 21 matched were analyzed. The HCQ/CQ treatment lasted for an average of 10.36±3.12 days. The VSPs were a little longer in the HCQ/ CQ treatment group (26.57±10.35 days vs. 19.10±7.80 days, P=0.020). There were 3 patients deceased during inpatient period, two patients were with HCQ/ CQ treatment (P=0.551). In the multivariate linear regression analysis, disease durations at admission (t=3.643, P=0.001) and HCQ/CQ treatment (t=2.637, P=0.013) were independent parameters for patients’ VSPs prediction. One patient with CQ had recurrent first-degree atrioventricular block (AVB) and obvious QTc elongation, another one complained about dizziness and blurred vision which disappeared after CQ discontinuation. One patient with HCQ had transient AVB.Conclusions: The HCQ/ CQ administration is not related to neither less mortality cases nor shorter VSPs. HCQ rather than CQ is relative safe and tolerable.

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Section: Discussionsupporting

confidence: 83%

Hydroxychloroquine/ Chloroquine in Patients With COVID-19 in Wuhan, China: A Retrospective Study

Chen

Liu

Cheng

et al. 2020

Preprint

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“…As explained in (Harris 2020a), these parameters give a basic reproductive number at the start of the epidemic equal to . The value of implies a mean duration of infectivity of 20 days, considerably larger than some estimates, but more in line with other data (Wölfel et al 2020, Xiao, Tong, andZhang 2020).…”

Section: Appendix: Sir Modelssupporting

confidence: 87%

Reopening Under COVID-19: What to Watch For

Harris¹

2020

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are gratefully acknowledged. The opinions expressed here are solely those of the author and do not represent the views of the Massachusetts Institute of Technology, Eisner Health, the National Bureau of Economic Research, or any other individual or organization. The author has received no direct or indirect remuneration for this article, and has no conflicts of interest to declare. This is the third article in a series. For the first two articles, see (Harris 2020a, b). The views expressed herein are those of the author and do not necessarily reflect the views of the National Bureau of Economic Research. NBER working papers are circulated for discussion and comment purposes. They have not been peerreviewed or been subject to the review by the NBER Board of Directors that accompanies official NBER publications.

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“…Some studies also reported that viral infectivity in patients with symptoms' duration > 8 days may be low [16,17]. Several factors might affect the occurrence of prolonged nucleic acid conversion, including older age, having comorbidities, such as diabetes and hypertension, and impaired immune function [18]. Our cases, except patient #1, had comorbidities, such as diabetes, hyperthyroid, asthma and arrhythmia.…”

Section: Discussionmentioning

confidence: 57%

Prolonged nucleic acid conversion and false-negative RT-PCR results in Indonesian patients with COVID-19: A case series

Trisnawati¹,

Khair²,

Fauzi³

et al. 2020

Preprint

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Background: Prolonged nucleic acid conversion and false-negative results of real-time reverse transcription polymerase chain reaction (RT-PCR) might occur in some patients with COVID-19 rather than recurrence of infection. Here, we reported four cases of COVID-19 with prolonged nucleic acid conversion and false-negative results of RT-PCR in our institution.Case presentation: Case 1: A 36-year-old-male patient complained of coughing up phlegm one week before admission. His chest X-rays showed mild pneumonia in the right lung. His swab test was confirmed positive for SARS-Cov-2. Besides the last two consecutive negative results, he also had negative results of RT-PCR twice (the 6th and 8th tests) from a total of 11 swab tests. Case 2: A 54-year-old-male patient complained of shortness of breath that worsened with activity. He had a comorbidity of diabetes. His chest X-rays showed inhomogeneous opacity on bilateral paracardial and lateral aspects. His swab test was confirmed positive for SARS-Cov-2. Besides the last two consecutive negative results, he also had negative results of RT-PCR once (the 5th test) from a total of 8 swab tests. Case 3: A 47-year-old man presented with complaints of fever, cough, sore throat, and diarrhea. He had comorbidities of asthma and heart rhythm disorders. His chest X-rays showed bilateral pneumonia. His swab test was confirmed positive for SARS-Cov-2. Besides the last two consecutive negative results, he also showed negative results of RT-PCR twice (the 4th and 6th tests) from a total of 11 swab tests. Case 4: A 56-year-old-female complained of lethargy and diarrhea. She has a history of hyperthyroidism. His chest X-rays showed bilateral pneumonia. She was confirmed positive for SARS-Cov-2. Besides the last two consecutive negative results, she also had negative results of RT-PCR twice (the 2nd and 10th tests) from a total of 14 swab tests.Conclusions: Our cases further confirmed the occurrence of prolonged nucleic acid conversion and the possibility of false negative results of RT-PCR in patients with COVID-19 instead of recurrence of infection. These findings might have an implication on the management of patients with COVID-19 who have already clinically and radiologically recovered, particularly related to subsequent spreading of the infection in the community.

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Profile of RT-PCR for SARS-CoV-2: A Preliminary Study From 56 COVID-19 Patients

Abstract: A novel coronavirus pandemic threatens the world. Here, we first studied the dynamics profile of SARS-CoV-2 from 56 recovered COVID-19 patients. We found virus shedding was up to 6 weeks after onset of symptoms. Prolonged observation period is necessary for older patients.

Cited by 237 publications

References 13 publications

Hydroxychloroquine/ Chloroquine in Patients With COVID-19 in Wuhan, China: A Retrospective Study

Hydroxychloroquine/ Chloroquine in Patients With COVID-19 in Wuhan, China: A Retrospective Study

Reopening Under COVID-19: What to Watch For

Prolonged nucleic acid conversion and false-negative RT-PCR results in Indonesian patients with COVID-19: A case series

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